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Leptin Enhances Nitric Oxide-Dependent Relaxation of the Clitoral Corpus Cavernosum
PURPOSE: The effects of leptin on female sexual behaviors are controversial, and studies on this topic are limited. The objectives of this study were to evaluate the direct effects of leptin on clitoral vasoreactivity in vitro and to determine the mechanism of action. MATERIALS AND METHODS: Isometri...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Korean Urological Association
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3045720/ https://www.ncbi.nlm.nih.gov/pubmed/21379432 http://dx.doi.org/10.4111/kju.2011.52.2.136 |
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author | Lee, Shin Young Chung, Woo Hyun Lee, Moo Yeol Kim, Sae Chul Kim, Hyun Woo Myung, Soon Chul |
author_facet | Lee, Shin Young Chung, Woo Hyun Lee, Moo Yeol Kim, Sae Chul Kim, Hyun Woo Myung, Soon Chul |
author_sort | Lee, Shin Young |
collection | PubMed |
description | PURPOSE: The effects of leptin on female sexual behaviors are controversial, and studies on this topic are limited. The objectives of this study were to evaluate the direct effects of leptin on clitoral vasoreactivity in vitro and to determine the mechanism of action. MATERIALS AND METHODS: Isometric tension studies were conducted to determine the effects of pretreatment with leptin (10(-8) M) on the contractile responses of rabbit clitoral corpus cavernosal smooth muscle strips. The effects of leptin were assessed on precontraction induced by phenylephrine (PE; 10(-9)-10(-4) M) and KCl (35-140 mM). We also examined the effect of leptin on relaxation induced by acetylcholine (ACh; 10(-9)-10(-4) M), verapamil (10(-10)-10(-6) M), and sodium nitroprusside (10(-9)-10(-4) M) in PE-precontracted (10(-5) M) strips. RESULTS: Leptin enhanced ACh-induced relaxation in PE-precontracted strips. L-NAME pretreatment significantly reduced the effect of leptin on ACh-induced relaxation, whereas L-arginine potentiated the effect of leptin. Leptin decreased the KCl-induced contractile responses. Leptin increased verapamil-induced relaxation responses. The relaxation effects of leptin on KCl-induced contraction were inhibited by 10(-5) M methylene blue and L-NAME pretreatment. CONCLUSIONS: A high concentration of leptin enhances ACh-dependent relaxation in clitoral cavernosal smooth muscles. These relaxation effects of leptin may occur through an NO-dependent mechanism and voltage-dependent calcium channel blockade. |
format | Text |
id | pubmed-3045720 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Korean Urological Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-30457202011-03-04 Leptin Enhances Nitric Oxide-Dependent Relaxation of the Clitoral Corpus Cavernosum Lee, Shin Young Chung, Woo Hyun Lee, Moo Yeol Kim, Sae Chul Kim, Hyun Woo Myung, Soon Chul Korean J Urol Original Article PURPOSE: The effects of leptin on female sexual behaviors are controversial, and studies on this topic are limited. The objectives of this study were to evaluate the direct effects of leptin on clitoral vasoreactivity in vitro and to determine the mechanism of action. MATERIALS AND METHODS: Isometric tension studies were conducted to determine the effects of pretreatment with leptin (10(-8) M) on the contractile responses of rabbit clitoral corpus cavernosal smooth muscle strips. The effects of leptin were assessed on precontraction induced by phenylephrine (PE; 10(-9)-10(-4) M) and KCl (35-140 mM). We also examined the effect of leptin on relaxation induced by acetylcholine (ACh; 10(-9)-10(-4) M), verapamil (10(-10)-10(-6) M), and sodium nitroprusside (10(-9)-10(-4) M) in PE-precontracted (10(-5) M) strips. RESULTS: Leptin enhanced ACh-induced relaxation in PE-precontracted strips. L-NAME pretreatment significantly reduced the effect of leptin on ACh-induced relaxation, whereas L-arginine potentiated the effect of leptin. Leptin decreased the KCl-induced contractile responses. Leptin increased verapamil-induced relaxation responses. The relaxation effects of leptin on KCl-induced contraction were inhibited by 10(-5) M methylene blue and L-NAME pretreatment. CONCLUSIONS: A high concentration of leptin enhances ACh-dependent relaxation in clitoral cavernosal smooth muscles. These relaxation effects of leptin may occur through an NO-dependent mechanism and voltage-dependent calcium channel blockade. The Korean Urological Association 2011-02 2011-02-21 /pmc/articles/PMC3045720/ /pubmed/21379432 http://dx.doi.org/10.4111/kju.2011.52.2.136 Text en © The Korean Urological Association, 2011 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Lee, Shin Young Chung, Woo Hyun Lee, Moo Yeol Kim, Sae Chul Kim, Hyun Woo Myung, Soon Chul Leptin Enhances Nitric Oxide-Dependent Relaxation of the Clitoral Corpus Cavernosum |
title | Leptin Enhances Nitric Oxide-Dependent Relaxation of the Clitoral Corpus Cavernosum |
title_full | Leptin Enhances Nitric Oxide-Dependent Relaxation of the Clitoral Corpus Cavernosum |
title_fullStr | Leptin Enhances Nitric Oxide-Dependent Relaxation of the Clitoral Corpus Cavernosum |
title_full_unstemmed | Leptin Enhances Nitric Oxide-Dependent Relaxation of the Clitoral Corpus Cavernosum |
title_short | Leptin Enhances Nitric Oxide-Dependent Relaxation of the Clitoral Corpus Cavernosum |
title_sort | leptin enhances nitric oxide-dependent relaxation of the clitoral corpus cavernosum |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3045720/ https://www.ncbi.nlm.nih.gov/pubmed/21379432 http://dx.doi.org/10.4111/kju.2011.52.2.136 |
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