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xnd-1 Regulates the Global Recombination Landscape in C. elegans

Meiotic crossover (CO) recombination establishes physical linkages between homologous chromosomes that are required for their proper segregation into developing gametes and promotes genetic diversity by shuffling genetic material between parental chromosomes. COs require the formation of double stra...

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Autores principales: Wagner, Cynthia R., Kuervers, Lynnette, Baillie, David, Yanowitz, Judith L.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3045774/
https://www.ncbi.nlm.nih.gov/pubmed/20944745
http://dx.doi.org/10.1038/nature09429
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author Wagner, Cynthia R.
Kuervers, Lynnette
Baillie, David
Yanowitz, Judith L.
author_facet Wagner, Cynthia R.
Kuervers, Lynnette
Baillie, David
Yanowitz, Judith L.
author_sort Wagner, Cynthia R.
collection PubMed
description Meiotic crossover (CO) recombination establishes physical linkages between homologous chromosomes that are required for their proper segregation into developing gametes and promotes genetic diversity by shuffling genetic material between parental chromosomes. COs require the formation of double strand breaks (DSBs) to create the substrate for strand exchange. DSBs occur in small intervals called hotspots1-3 and significant variation in hotspot usage exists between and among individuals4. This variation is thought to reflect differences in sequence identity and chromatin structure, DNA topology and/ or chromosome domain organization1, 5-9. Chromosomes show different frequencies of nondisjunction (NDJ)10, reflecting inherent differences in meiotic crossover control, yet the underlying basis of these differences remains elusive. Here we show that a novel chromatin factor, X non-disjunction factor 1 (xnd-1), is responsible for the global distribution of COs in C. elegans. xnd-1 is also required for formation of double-strand breaks (DSBs) on the X, but surprisingly XND-1 protein is autosomally-enriched. We show that xnd-1 functions independently of genes required for X chromosome-specific gene silencing, revealing a novel pathway that distinguishes the X from autosomes in the germ line, and further show that xnd-1 exerts its effects on COs, at least in part, by modulating levels of H2A lysine 5 acetylation.
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spelling pubmed-30457742011-04-14 xnd-1 Regulates the Global Recombination Landscape in C. elegans Wagner, Cynthia R. Kuervers, Lynnette Baillie, David Yanowitz, Judith L. Nature Article Meiotic crossover (CO) recombination establishes physical linkages between homologous chromosomes that are required for their proper segregation into developing gametes and promotes genetic diversity by shuffling genetic material between parental chromosomes. COs require the formation of double strand breaks (DSBs) to create the substrate for strand exchange. DSBs occur in small intervals called hotspots1-3 and significant variation in hotspot usage exists between and among individuals4. This variation is thought to reflect differences in sequence identity and chromatin structure, DNA topology and/ or chromosome domain organization1, 5-9. Chromosomes show different frequencies of nondisjunction (NDJ)10, reflecting inherent differences in meiotic crossover control, yet the underlying basis of these differences remains elusive. Here we show that a novel chromatin factor, X non-disjunction factor 1 (xnd-1), is responsible for the global distribution of COs in C. elegans. xnd-1 is also required for formation of double-strand breaks (DSBs) on the X, but surprisingly XND-1 protein is autosomally-enriched. We show that xnd-1 functions independently of genes required for X chromosome-specific gene silencing, revealing a novel pathway that distinguishes the X from autosomes in the germ line, and further show that xnd-1 exerts its effects on COs, at least in part, by modulating levels of H2A lysine 5 acetylation. 2010-10-14 /pmc/articles/PMC3045774/ /pubmed/20944745 http://dx.doi.org/10.1038/nature09429 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Wagner, Cynthia R.
Kuervers, Lynnette
Baillie, David
Yanowitz, Judith L.
xnd-1 Regulates the Global Recombination Landscape in C. elegans
title xnd-1 Regulates the Global Recombination Landscape in C. elegans
title_full xnd-1 Regulates the Global Recombination Landscape in C. elegans
title_fullStr xnd-1 Regulates the Global Recombination Landscape in C. elegans
title_full_unstemmed xnd-1 Regulates the Global Recombination Landscape in C. elegans
title_short xnd-1 Regulates the Global Recombination Landscape in C. elegans
title_sort xnd-1 regulates the global recombination landscape in c. elegans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3045774/
https://www.ncbi.nlm.nih.gov/pubmed/20944745
http://dx.doi.org/10.1038/nature09429
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