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Biogenic Volatile Organic Compound and Respiratory CO(2) Emissions after (13)C-Labeling: Online Tracing of C Translocation Dynamics in Poplar Plants

BACKGROUND: Globally plants are the primary sink of atmospheric CO(2), but are also the major contributor of a large spectrum of atmospheric reactive hydrocarbons such as terpenes (e.g. isoprene) and other biogenic volatile organic compounds (BVOC). The prediction of plant carbon (C) uptake and atmo...

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Autores principales: Ghirardo, Andrea, Gutknecht, Jessica, Zimmer, Ina, Brüggemann, Nicolas, Schnitzler, Jörg-Peter
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3046154/
https://www.ncbi.nlm.nih.gov/pubmed/21387007
http://dx.doi.org/10.1371/journal.pone.0017393
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author Ghirardo, Andrea
Gutknecht, Jessica
Zimmer, Ina
Brüggemann, Nicolas
Schnitzler, Jörg-Peter
author_facet Ghirardo, Andrea
Gutknecht, Jessica
Zimmer, Ina
Brüggemann, Nicolas
Schnitzler, Jörg-Peter
author_sort Ghirardo, Andrea
collection PubMed
description BACKGROUND: Globally plants are the primary sink of atmospheric CO(2), but are also the major contributor of a large spectrum of atmospheric reactive hydrocarbons such as terpenes (e.g. isoprene) and other biogenic volatile organic compounds (BVOC). The prediction of plant carbon (C) uptake and atmospheric oxidation capacity are crucial to define the trajectory and consequences of global environmental changes. To achieve this, the biosynthesis of BVOC and the dynamics of C allocation and translocation in both plants and ecosystems are important. METHODOLOGY: We combined tunable diode laser absorption spectrometry (TDLAS) and proton transfer reaction mass spectrometry (PTR-MS) for studying isoprene biosynthesis and following C fluxes within grey poplar (Populus x canescens) saplings. This was achieved by feeding either (13)CO(2) to leaves or (13)C-glucose to shoots via xylem uptake. The translocation of (13)CO(2) from the source to other plant parts could be traced by (13)C-labeled isoprene and respiratory (13)CO(2) emission. PRINCIPAL FINDING: In intact plants, assimilated (13)CO(2) was rapidly translocated via the phloem to the roots within 1 hour, with an average phloem transport velocity of 20.3±2.5 cm h(−1). (13)C label was stored in the roots and partially reallocated to the plants' apical part one day after labeling, particularly in the absence of photosynthesis. The daily C loss as BVOC ranged between 1.6% in mature leaves and 7.0% in young leaves. Non-isoprene BVOC accounted under light conditions for half of the BVOC C loss in young leaves and one-third in mature leaves. The C loss as isoprene originated mainly (76–78%) from recently fixed CO(2), to a minor extent from xylem-transported sugars (7–11%) and from photosynthetic intermediates with slower turnover rates (8–11%). CONCLUSION: We quantified the plants' C loss as respiratory CO(2) and BVOC emissions, allowing in tandem with metabolic analysis to deepen our understanding of ecosystem C flux.
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spelling pubmed-30461542011-03-08 Biogenic Volatile Organic Compound and Respiratory CO(2) Emissions after (13)C-Labeling: Online Tracing of C Translocation Dynamics in Poplar Plants Ghirardo, Andrea Gutknecht, Jessica Zimmer, Ina Brüggemann, Nicolas Schnitzler, Jörg-Peter PLoS One Research Article BACKGROUND: Globally plants are the primary sink of atmospheric CO(2), but are also the major contributor of a large spectrum of atmospheric reactive hydrocarbons such as terpenes (e.g. isoprene) and other biogenic volatile organic compounds (BVOC). The prediction of plant carbon (C) uptake and atmospheric oxidation capacity are crucial to define the trajectory and consequences of global environmental changes. To achieve this, the biosynthesis of BVOC and the dynamics of C allocation and translocation in both plants and ecosystems are important. METHODOLOGY: We combined tunable diode laser absorption spectrometry (TDLAS) and proton transfer reaction mass spectrometry (PTR-MS) for studying isoprene biosynthesis and following C fluxes within grey poplar (Populus x canescens) saplings. This was achieved by feeding either (13)CO(2) to leaves or (13)C-glucose to shoots via xylem uptake. The translocation of (13)CO(2) from the source to other plant parts could be traced by (13)C-labeled isoprene and respiratory (13)CO(2) emission. PRINCIPAL FINDING: In intact plants, assimilated (13)CO(2) was rapidly translocated via the phloem to the roots within 1 hour, with an average phloem transport velocity of 20.3±2.5 cm h(−1). (13)C label was stored in the roots and partially reallocated to the plants' apical part one day after labeling, particularly in the absence of photosynthesis. The daily C loss as BVOC ranged between 1.6% in mature leaves and 7.0% in young leaves. Non-isoprene BVOC accounted under light conditions for half of the BVOC C loss in young leaves and one-third in mature leaves. The C loss as isoprene originated mainly (76–78%) from recently fixed CO(2), to a minor extent from xylem-transported sugars (7–11%) and from photosynthetic intermediates with slower turnover rates (8–11%). CONCLUSION: We quantified the plants' C loss as respiratory CO(2) and BVOC emissions, allowing in tandem with metabolic analysis to deepen our understanding of ecosystem C flux. Public Library of Science 2011-02-28 /pmc/articles/PMC3046154/ /pubmed/21387007 http://dx.doi.org/10.1371/journal.pone.0017393 Text en Ghirardo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ghirardo, Andrea
Gutknecht, Jessica
Zimmer, Ina
Brüggemann, Nicolas
Schnitzler, Jörg-Peter
Biogenic Volatile Organic Compound and Respiratory CO(2) Emissions after (13)C-Labeling: Online Tracing of C Translocation Dynamics in Poplar Plants
title Biogenic Volatile Organic Compound and Respiratory CO(2) Emissions after (13)C-Labeling: Online Tracing of C Translocation Dynamics in Poplar Plants
title_full Biogenic Volatile Organic Compound and Respiratory CO(2) Emissions after (13)C-Labeling: Online Tracing of C Translocation Dynamics in Poplar Plants
title_fullStr Biogenic Volatile Organic Compound and Respiratory CO(2) Emissions after (13)C-Labeling: Online Tracing of C Translocation Dynamics in Poplar Plants
title_full_unstemmed Biogenic Volatile Organic Compound and Respiratory CO(2) Emissions after (13)C-Labeling: Online Tracing of C Translocation Dynamics in Poplar Plants
title_short Biogenic Volatile Organic Compound and Respiratory CO(2) Emissions after (13)C-Labeling: Online Tracing of C Translocation Dynamics in Poplar Plants
title_sort biogenic volatile organic compound and respiratory co(2) emissions after (13)c-labeling: online tracing of c translocation dynamics in poplar plants
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3046154/
https://www.ncbi.nlm.nih.gov/pubmed/21387007
http://dx.doi.org/10.1371/journal.pone.0017393
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