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In vivo molecular imaging of experimental joint inflammation by combined (18)F-FDG positron emission tomography and computed tomography

INTRODUCTION: The purpose of this work was to establish and validate combined small animal positron emission tomography - computed tomography (PET/CT) as a new in vivo imaging method for visualisation and quantification of joint inflammation. METHODS: Signalling of radioisotope (18)F labelled Fluoro...

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Autores principales: Irmler, Ingo M, Opfermann, Thomas, Gebhardt, Peter, Gajda, Mieczyslaw, Bräuer, Rolf, Saluz, Hans P, Kamradt, Thomas
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3046507/
https://www.ncbi.nlm.nih.gov/pubmed/21047399
http://dx.doi.org/10.1186/ar3176
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author Irmler, Ingo M
Opfermann, Thomas
Gebhardt, Peter
Gajda, Mieczyslaw
Bräuer, Rolf
Saluz, Hans P
Kamradt, Thomas
author_facet Irmler, Ingo M
Opfermann, Thomas
Gebhardt, Peter
Gajda, Mieczyslaw
Bräuer, Rolf
Saluz, Hans P
Kamradt, Thomas
author_sort Irmler, Ingo M
collection PubMed
description INTRODUCTION: The purpose of this work was to establish and validate combined small animal positron emission tomography - computed tomography (PET/CT) as a new in vivo imaging method for visualisation and quantification of joint inflammation. METHODS: Signalling of radioisotope (18)F labelled Fluorodeoxyglucose ((18)F-FDG) injected in mice with glucose-6-phosphate isomerase (G6PI)-induced arthritis was analysed by PET/CT. Accumulation of (18)F-FDG in tissue was quantified by PET measurement, whereas high definition CT delivered anatomical information. The fusion of both images revealed in detail spatial and temporal distribution and metabolism of (18)F-FDG. RESULTS: A distinct (18)F-FDG signal could be measured by PET in carpal and tarsal joints, from mice with early or established arthritis. In contrast, no accumulation of (18)F-FDG was detectable before arthritis onset. Comparison of (18)F-FDG joint uptake with histopathological evaluation revealed a significant correlation of both methods. CONCLUSIONS: Small animal PET/CT using (18)F-FDG is a feasible method for monitoring and, more importantly, quantitative assessment of inflammation in G6PI-arthritis. Since it is possible to perform repeated non-invasive measurements in vivo, not only numbers of animals in preclinical studies can markedly be reduced by this method, but also longitudinal studies come into reach, e. g. for individual flare-up reactions or monitoring therapy response in progressive arthritis.
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spelling pubmed-30465072011-03-01 In vivo molecular imaging of experimental joint inflammation by combined (18)F-FDG positron emission tomography and computed tomography Irmler, Ingo M Opfermann, Thomas Gebhardt, Peter Gajda, Mieczyslaw Bräuer, Rolf Saluz, Hans P Kamradt, Thomas Arthritis Res Ther Research Article INTRODUCTION: The purpose of this work was to establish and validate combined small animal positron emission tomography - computed tomography (PET/CT) as a new in vivo imaging method for visualisation and quantification of joint inflammation. METHODS: Signalling of radioisotope (18)F labelled Fluorodeoxyglucose ((18)F-FDG) injected in mice with glucose-6-phosphate isomerase (G6PI)-induced arthritis was analysed by PET/CT. Accumulation of (18)F-FDG in tissue was quantified by PET measurement, whereas high definition CT delivered anatomical information. The fusion of both images revealed in detail spatial and temporal distribution and metabolism of (18)F-FDG. RESULTS: A distinct (18)F-FDG signal could be measured by PET in carpal and tarsal joints, from mice with early or established arthritis. In contrast, no accumulation of (18)F-FDG was detectable before arthritis onset. Comparison of (18)F-FDG joint uptake with histopathological evaluation revealed a significant correlation of both methods. CONCLUSIONS: Small animal PET/CT using (18)F-FDG is a feasible method for monitoring and, more importantly, quantitative assessment of inflammation in G6PI-arthritis. Since it is possible to perform repeated non-invasive measurements in vivo, not only numbers of animals in preclinical studies can markedly be reduced by this method, but also longitudinal studies come into reach, e. g. for individual flare-up reactions or monitoring therapy response in progressive arthritis. BioMed Central 2010 2010-11-03 /pmc/articles/PMC3046507/ /pubmed/21047399 http://dx.doi.org/10.1186/ar3176 Text en Copyright ©2010 Irmler et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Irmler, Ingo M
Opfermann, Thomas
Gebhardt, Peter
Gajda, Mieczyslaw
Bräuer, Rolf
Saluz, Hans P
Kamradt, Thomas
In vivo molecular imaging of experimental joint inflammation by combined (18)F-FDG positron emission tomography and computed tomography
title In vivo molecular imaging of experimental joint inflammation by combined (18)F-FDG positron emission tomography and computed tomography
title_full In vivo molecular imaging of experimental joint inflammation by combined (18)F-FDG positron emission tomography and computed tomography
title_fullStr In vivo molecular imaging of experimental joint inflammation by combined (18)F-FDG positron emission tomography and computed tomography
title_full_unstemmed In vivo molecular imaging of experimental joint inflammation by combined (18)F-FDG positron emission tomography and computed tomography
title_short In vivo molecular imaging of experimental joint inflammation by combined (18)F-FDG positron emission tomography and computed tomography
title_sort in vivo molecular imaging of experimental joint inflammation by combined (18)f-fdg positron emission tomography and computed tomography
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3046507/
https://www.ncbi.nlm.nih.gov/pubmed/21047399
http://dx.doi.org/10.1186/ar3176
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