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In vivo molecular imaging of experimental joint inflammation by combined (18)F-FDG positron emission tomography and computed tomography
INTRODUCTION: The purpose of this work was to establish and validate combined small animal positron emission tomography - computed tomography (PET/CT) as a new in vivo imaging method for visualisation and quantification of joint inflammation. METHODS: Signalling of radioisotope (18)F labelled Fluoro...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3046507/ https://www.ncbi.nlm.nih.gov/pubmed/21047399 http://dx.doi.org/10.1186/ar3176 |
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author | Irmler, Ingo M Opfermann, Thomas Gebhardt, Peter Gajda, Mieczyslaw Bräuer, Rolf Saluz, Hans P Kamradt, Thomas |
author_facet | Irmler, Ingo M Opfermann, Thomas Gebhardt, Peter Gajda, Mieczyslaw Bräuer, Rolf Saluz, Hans P Kamradt, Thomas |
author_sort | Irmler, Ingo M |
collection | PubMed |
description | INTRODUCTION: The purpose of this work was to establish and validate combined small animal positron emission tomography - computed tomography (PET/CT) as a new in vivo imaging method for visualisation and quantification of joint inflammation. METHODS: Signalling of radioisotope (18)F labelled Fluorodeoxyglucose ((18)F-FDG) injected in mice with glucose-6-phosphate isomerase (G6PI)-induced arthritis was analysed by PET/CT. Accumulation of (18)F-FDG in tissue was quantified by PET measurement, whereas high definition CT delivered anatomical information. The fusion of both images revealed in detail spatial and temporal distribution and metabolism of (18)F-FDG. RESULTS: A distinct (18)F-FDG signal could be measured by PET in carpal and tarsal joints, from mice with early or established arthritis. In contrast, no accumulation of (18)F-FDG was detectable before arthritis onset. Comparison of (18)F-FDG joint uptake with histopathological evaluation revealed a significant correlation of both methods. CONCLUSIONS: Small animal PET/CT using (18)F-FDG is a feasible method for monitoring and, more importantly, quantitative assessment of inflammation in G6PI-arthritis. Since it is possible to perform repeated non-invasive measurements in vivo, not only numbers of animals in preclinical studies can markedly be reduced by this method, but also longitudinal studies come into reach, e. g. for individual flare-up reactions or monitoring therapy response in progressive arthritis. |
format | Text |
id | pubmed-3046507 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30465072011-03-01 In vivo molecular imaging of experimental joint inflammation by combined (18)F-FDG positron emission tomography and computed tomography Irmler, Ingo M Opfermann, Thomas Gebhardt, Peter Gajda, Mieczyslaw Bräuer, Rolf Saluz, Hans P Kamradt, Thomas Arthritis Res Ther Research Article INTRODUCTION: The purpose of this work was to establish and validate combined small animal positron emission tomography - computed tomography (PET/CT) as a new in vivo imaging method for visualisation and quantification of joint inflammation. METHODS: Signalling of radioisotope (18)F labelled Fluorodeoxyglucose ((18)F-FDG) injected in mice with glucose-6-phosphate isomerase (G6PI)-induced arthritis was analysed by PET/CT. Accumulation of (18)F-FDG in tissue was quantified by PET measurement, whereas high definition CT delivered anatomical information. The fusion of both images revealed in detail spatial and temporal distribution and metabolism of (18)F-FDG. RESULTS: A distinct (18)F-FDG signal could be measured by PET in carpal and tarsal joints, from mice with early or established arthritis. In contrast, no accumulation of (18)F-FDG was detectable before arthritis onset. Comparison of (18)F-FDG joint uptake with histopathological evaluation revealed a significant correlation of both methods. CONCLUSIONS: Small animal PET/CT using (18)F-FDG is a feasible method for monitoring and, more importantly, quantitative assessment of inflammation in G6PI-arthritis. Since it is possible to perform repeated non-invasive measurements in vivo, not only numbers of animals in preclinical studies can markedly be reduced by this method, but also longitudinal studies come into reach, e. g. for individual flare-up reactions or monitoring therapy response in progressive arthritis. BioMed Central 2010 2010-11-03 /pmc/articles/PMC3046507/ /pubmed/21047399 http://dx.doi.org/10.1186/ar3176 Text en Copyright ©2010 Irmler et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Irmler, Ingo M Opfermann, Thomas Gebhardt, Peter Gajda, Mieczyslaw Bräuer, Rolf Saluz, Hans P Kamradt, Thomas In vivo molecular imaging of experimental joint inflammation by combined (18)F-FDG positron emission tomography and computed tomography |
title | In vivo molecular imaging of experimental joint inflammation by combined (18)F-FDG positron emission tomography and computed tomography |
title_full | In vivo molecular imaging of experimental joint inflammation by combined (18)F-FDG positron emission tomography and computed tomography |
title_fullStr | In vivo molecular imaging of experimental joint inflammation by combined (18)F-FDG positron emission tomography and computed tomography |
title_full_unstemmed | In vivo molecular imaging of experimental joint inflammation by combined (18)F-FDG positron emission tomography and computed tomography |
title_short | In vivo molecular imaging of experimental joint inflammation by combined (18)F-FDG positron emission tomography and computed tomography |
title_sort | in vivo molecular imaging of experimental joint inflammation by combined (18)f-fdg positron emission tomography and computed tomography |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3046507/ https://www.ncbi.nlm.nih.gov/pubmed/21047399 http://dx.doi.org/10.1186/ar3176 |
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