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Nanometer-scale optical imaging of collagen fibers using gold nanoparticles

We describe 3D single particle tracking of gold nanoparticles (AuNPs) moving along collagen fibers in aqueous environment with two-photon excitation conditions. The photoacoustic effect at the collagen fiber caused by the irradiation with ultrashort, near-infrared laser pulses propels the particles...

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Detalles Bibliográficos
Autores principales: Chen, Bo, Estrada, Laura C., Hellriegel, Christian, Gratton, Enrico
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3047357/
https://www.ncbi.nlm.nih.gov/pubmed/21412457
http://dx.doi.org/10.1364/BOE.2.000511
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author Chen, Bo
Estrada, Laura C.
Hellriegel, Christian
Gratton, Enrico
author_facet Chen, Bo
Estrada, Laura C.
Hellriegel, Christian
Gratton, Enrico
author_sort Chen, Bo
collection PubMed
description We describe 3D single particle tracking of gold nanoparticles (AuNPs) moving along collagen fibers in aqueous environment with two-photon excitation conditions. The photoacoustic effect at the collagen fiber caused by the irradiation with ultrashort, near-infrared laser pulses propels the particles adsorbed to the surface of the collagen fibers. We report the tracking of individual AuNPs in three dimensions with high spatial and temporal resolution, of few nanometers and milliseconds, respectively. Due to the emission signal caused by the interaction between the AuNPs and the weak chromophores in the collagen fiber, the trajectories of individual AuNPs reveal the fiber topography with nanometric resolution. The intensity along the trajectory shows that we are sensitive to the distribution of the weak chromophores on the fiber.
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spelling pubmed-30473572011-03-16 Nanometer-scale optical imaging of collagen fibers using gold nanoparticles Chen, Bo Estrada, Laura C. Hellriegel, Christian Gratton, Enrico Biomed Opt Express Microscopy We describe 3D single particle tracking of gold nanoparticles (AuNPs) moving along collagen fibers in aqueous environment with two-photon excitation conditions. The photoacoustic effect at the collagen fiber caused by the irradiation with ultrashort, near-infrared laser pulses propels the particles adsorbed to the surface of the collagen fibers. We report the tracking of individual AuNPs in three dimensions with high spatial and temporal resolution, of few nanometers and milliseconds, respectively. Due to the emission signal caused by the interaction between the AuNPs and the weak chromophores in the collagen fiber, the trajectories of individual AuNPs reveal the fiber topography with nanometric resolution. The intensity along the trajectory shows that we are sensitive to the distribution of the weak chromophores on the fiber. Optical Society of America 2011-02-07 /pmc/articles/PMC3047357/ /pubmed/21412457 http://dx.doi.org/10.1364/BOE.2.000511 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Microscopy
Chen, Bo
Estrada, Laura C.
Hellriegel, Christian
Gratton, Enrico
Nanometer-scale optical imaging of collagen fibers using gold nanoparticles
title Nanometer-scale optical imaging of collagen fibers using gold nanoparticles
title_full Nanometer-scale optical imaging of collagen fibers using gold nanoparticles
title_fullStr Nanometer-scale optical imaging of collagen fibers using gold nanoparticles
title_full_unstemmed Nanometer-scale optical imaging of collagen fibers using gold nanoparticles
title_short Nanometer-scale optical imaging of collagen fibers using gold nanoparticles
title_sort nanometer-scale optical imaging of collagen fibers using gold nanoparticles
topic Microscopy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3047357/
https://www.ncbi.nlm.nih.gov/pubmed/21412457
http://dx.doi.org/10.1364/BOE.2.000511
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AT grattonenrico nanometerscaleopticalimagingofcollagenfibersusinggoldnanoparticles