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Volumetric and quantitative imaging of retinal blood flow in rats with optical microangiography

In this paper, we present methods for 3D visualization and quantitative measurements of retinal blood flow in rats by the use of optical microangiography imaging technique (OMAG). We use ultrahigh sensitive OMAG to provide high-quality 3D RBF perfusion maps in the rat eye, from which the Doppler ang...

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Autores principales: Zhi, Zhongwei, Cepurna, William, Johnson, Elaine, Shen, Tueng, Morrison, John, Wang, Ruikang K
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3047363/
https://www.ncbi.nlm.nih.gov/pubmed/21412463
http://dx.doi.org/10.1364/BOE.2.000579
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author Zhi, Zhongwei
Cepurna, William
Johnson, Elaine
Shen, Tueng
Morrison, John
Wang, Ruikang K
author_facet Zhi, Zhongwei
Cepurna, William
Johnson, Elaine
Shen, Tueng
Morrison, John
Wang, Ruikang K
author_sort Zhi, Zhongwei
collection PubMed
description In this paper, we present methods for 3D visualization and quantitative measurements of retinal blood flow in rats by the use of optical microangiography imaging technique (OMAG). We use ultrahigh sensitive OMAG to provide high-quality 3D RBF perfusion maps in the rat eye, from which the Doppler angle, as well as the diameters of blood vessels, are evaluated. Estimation of flow velocity (i.e. axial flow velocity) is achieved by the use of Doppler OMAG, which has its origins in phase-resolved Doppler optical coherence tomography. The measurements of the Doppler angle, vessel size, and the axial velocity lead to the quantitative assessment of the absolute flow velocity and the blood flow rate in selected retinal vessels. We demonstrate the feasibility of OMAG to provide 3D microangiograms and quantitative assessment of retinal blood flow in a rat model subjected to raised intra-ocular pressure (IOP). We show that OMAG is capable of monitoring the longitudinal response of absolute blood velocity and flow rate of retinal blood vessels to increased IOP in the rat, demonstrating its usefulness for ophthalmological research.
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spelling pubmed-30473632011-03-16 Volumetric and quantitative imaging of retinal blood flow in rats with optical microangiography Zhi, Zhongwei Cepurna, William Johnson, Elaine Shen, Tueng Morrison, John Wang, Ruikang K Biomed Opt Express Optical Coherence Tomography In this paper, we present methods for 3D visualization and quantitative measurements of retinal blood flow in rats by the use of optical microangiography imaging technique (OMAG). We use ultrahigh sensitive OMAG to provide high-quality 3D RBF perfusion maps in the rat eye, from which the Doppler angle, as well as the diameters of blood vessels, are evaluated. Estimation of flow velocity (i.e. axial flow velocity) is achieved by the use of Doppler OMAG, which has its origins in phase-resolved Doppler optical coherence tomography. The measurements of the Doppler angle, vessel size, and the axial velocity lead to the quantitative assessment of the absolute flow velocity and the blood flow rate in selected retinal vessels. We demonstrate the feasibility of OMAG to provide 3D microangiograms and quantitative assessment of retinal blood flow in a rat model subjected to raised intra-ocular pressure (IOP). We show that OMAG is capable of monitoring the longitudinal response of absolute blood velocity and flow rate of retinal blood vessels to increased IOP in the rat, demonstrating its usefulness for ophthalmological research. Optical Society of America 2011-02-15 /pmc/articles/PMC3047363/ /pubmed/21412463 http://dx.doi.org/10.1364/BOE.2.000579 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Optical Coherence Tomography
Zhi, Zhongwei
Cepurna, William
Johnson, Elaine
Shen, Tueng
Morrison, John
Wang, Ruikang K
Volumetric and quantitative imaging of retinal blood flow in rats with optical microangiography
title Volumetric and quantitative imaging of retinal blood flow in rats with optical microangiography
title_full Volumetric and quantitative imaging of retinal blood flow in rats with optical microangiography
title_fullStr Volumetric and quantitative imaging of retinal blood flow in rats with optical microangiography
title_full_unstemmed Volumetric and quantitative imaging of retinal blood flow in rats with optical microangiography
title_short Volumetric and quantitative imaging of retinal blood flow in rats with optical microangiography
title_sort volumetric and quantitative imaging of retinal blood flow in rats with optical microangiography
topic Optical Coherence Tomography
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3047363/
https://www.ncbi.nlm.nih.gov/pubmed/21412463
http://dx.doi.org/10.1364/BOE.2.000579
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