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Cellular immune responses in patients with hepatitis B surface antigen seroclearance induced by antiviral therapy
BACKGROUND: The mechanisms by which chronic hepatitis B is completely resolved through antiviral therapy are unknown, and the contribution of acquired T cell immunity to hepatitis B surface antigen (HBsAg) seroclearance has not been investigated. Therefore, we measured the T-cell responses to core a...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3049122/ https://www.ncbi.nlm.nih.gov/pubmed/21320337 http://dx.doi.org/10.1186/1743-422X-8-69 |
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author | Liang, Minfeng Ma, Shiwu Hu, Xiaoxiong Zhou, Bin Zhang, Junchang Chen, Jinjun Wang, Zhanhui Sun, Jian Zhu, Xiaolin Abbott, William Hou, Jinlin |
author_facet | Liang, Minfeng Ma, Shiwu Hu, Xiaoxiong Zhou, Bin Zhang, Junchang Chen, Jinjun Wang, Zhanhui Sun, Jian Zhu, Xiaolin Abbott, William Hou, Jinlin |
author_sort | Liang, Minfeng |
collection | PubMed |
description | BACKGROUND: The mechanisms by which chronic hepatitis B is completely resolved through antiviral therapy are unknown, and the contribution of acquired T cell immunity to hepatitis B surface antigen (HBsAg) seroclearance has not been investigated. Therefore, we measured the T-cell responses to core and envelope antigens in patients with HBsAg seroclearance. METHODS: Fourteen subjects with HBsAg seroclearance following antiviral treatment for chronic hepatitis B, 7 HBeAg-positive immunotolerant HBV carriers and 9 HBeAg-negative inactive HBsAg carriers were recruited. HBV-specific T-cell responses to recombinant HBV core (rHBcAg) and envelope (rHBsAg) proteins and pools of core and envelope peptides were measured using an ELISPOT assay detecting interferon-gamma and intracellular cytokine staining (ICS) assays detecting interferon-gamma or interleukin 2. RESULTS: Interferon-gamma ELISPOT assays showed a low frequency of weak responses to the rHBsAg and S peptide pool in the HBsAg seroclearance group, and the response frequency to the rHBcAg and the C peptide pool was higher than to the rHBsAg (P < 0.001) and S peptide pool (P = 0.001) respectively. A higher response frequency to C than S peptide pools was confirmed in the interferon-gamma ICS assays for both CD4+ (P = 0.033) and CD8+ (P = 0.040) T cells in the HBsAg seroclearance group. The responses to C and S antigens in the inactive carriers were similar. CONCLUSIONS: There was a low frequency of CD4+ and CD8+ T cell immune responses to envelope antigens in Chinese subjects with HBsAg seroclearance following antiviral therapy. It is unlikely that these immune responses are responsible for HBsAg seroclearance in these subjects. |
format | Text |
id | pubmed-3049122 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30491222011-03-06 Cellular immune responses in patients with hepatitis B surface antigen seroclearance induced by antiviral therapy Liang, Minfeng Ma, Shiwu Hu, Xiaoxiong Zhou, Bin Zhang, Junchang Chen, Jinjun Wang, Zhanhui Sun, Jian Zhu, Xiaolin Abbott, William Hou, Jinlin Virol J Research BACKGROUND: The mechanisms by which chronic hepatitis B is completely resolved through antiviral therapy are unknown, and the contribution of acquired T cell immunity to hepatitis B surface antigen (HBsAg) seroclearance has not been investigated. Therefore, we measured the T-cell responses to core and envelope antigens in patients with HBsAg seroclearance. METHODS: Fourteen subjects with HBsAg seroclearance following antiviral treatment for chronic hepatitis B, 7 HBeAg-positive immunotolerant HBV carriers and 9 HBeAg-negative inactive HBsAg carriers were recruited. HBV-specific T-cell responses to recombinant HBV core (rHBcAg) and envelope (rHBsAg) proteins and pools of core and envelope peptides were measured using an ELISPOT assay detecting interferon-gamma and intracellular cytokine staining (ICS) assays detecting interferon-gamma or interleukin 2. RESULTS: Interferon-gamma ELISPOT assays showed a low frequency of weak responses to the rHBsAg and S peptide pool in the HBsAg seroclearance group, and the response frequency to the rHBcAg and the C peptide pool was higher than to the rHBsAg (P < 0.001) and S peptide pool (P = 0.001) respectively. A higher response frequency to C than S peptide pools was confirmed in the interferon-gamma ICS assays for both CD4+ (P = 0.033) and CD8+ (P = 0.040) T cells in the HBsAg seroclearance group. The responses to C and S antigens in the inactive carriers were similar. CONCLUSIONS: There was a low frequency of CD4+ and CD8+ T cell immune responses to envelope antigens in Chinese subjects with HBsAg seroclearance following antiviral therapy. It is unlikely that these immune responses are responsible for HBsAg seroclearance in these subjects. BioMed Central 2011-02-14 /pmc/articles/PMC3049122/ /pubmed/21320337 http://dx.doi.org/10.1186/1743-422X-8-69 Text en Copyright ©2011 Liang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Liang, Minfeng Ma, Shiwu Hu, Xiaoxiong Zhou, Bin Zhang, Junchang Chen, Jinjun Wang, Zhanhui Sun, Jian Zhu, Xiaolin Abbott, William Hou, Jinlin Cellular immune responses in patients with hepatitis B surface antigen seroclearance induced by antiviral therapy |
title | Cellular immune responses in patients with hepatitis B surface antigen seroclearance induced by antiviral therapy |
title_full | Cellular immune responses in patients with hepatitis B surface antigen seroclearance induced by antiviral therapy |
title_fullStr | Cellular immune responses in patients with hepatitis B surface antigen seroclearance induced by antiviral therapy |
title_full_unstemmed | Cellular immune responses in patients with hepatitis B surface antigen seroclearance induced by antiviral therapy |
title_short | Cellular immune responses in patients with hepatitis B surface antigen seroclearance induced by antiviral therapy |
title_sort | cellular immune responses in patients with hepatitis b surface antigen seroclearance induced by antiviral therapy |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3049122/ https://www.ncbi.nlm.nih.gov/pubmed/21320337 http://dx.doi.org/10.1186/1743-422X-8-69 |
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