100% complete assignment of non-labile (1)H, (13)C, and (15)N signals for calcium-loaded calbindin D(9k) P43G

Here we present the 100% complete assignment chemical shift of non-labile (1)H, (15)N and (13)C nuclei of Calbindin D(9k) P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysNζ as well as the termini. NMR experiments required to a...

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Detalles Bibliográficos
Autores principales: Oktaviani, Nur Alia, Otten, Renee, Dijkstra, Klaas, Scheek, Ruud M., Thulin, Eva, Akke, Mikael, Mulder, Frans A. A.
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3049223/
https://www.ncbi.nlm.nih.gov/pubmed/21069485
http://dx.doi.org/10.1007/s12104-010-9272-3
Descripción
Sumario:Here we present the 100% complete assignment chemical shift of non-labile (1)H, (15)N and (13)C nuclei of Calbindin D(9k) P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysNζ as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Calbindin D(9k) extend beyond previous studies reaching near-completeness (Vis et al. in Biochem 33:14858–14870, 1994; Yamazaki et al. in J Am Chem Soc 116:6464–6465, 1994; Yamazaki et al. in Biochem 32:5656–5669, 1993b).

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