100% complete assignment of non-labile (1)H, (13)C, and (15)N signals for calcium-loaded calbindin D(9k) P43G

Here we present the 100% complete assignment chemical shift of non-labile (1)H, (15)N and (13)C nuclei of Calbindin D(9k) P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysNζ as well as the termini. NMR experiments required to a...

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Autores principales: Oktaviani, Nur Alia, Otten, Renee, Dijkstra, Klaas, Scheek, Ruud M., Thulin, Eva, Akke, Mikael, Mulder, Frans A. A.
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3049223/
https://www.ncbi.nlm.nih.gov/pubmed/21069485
http://dx.doi.org/10.1007/s12104-010-9272-3
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author Oktaviani, Nur Alia
Otten, Renee
Dijkstra, Klaas
Scheek, Ruud M.
Thulin, Eva
Akke, Mikael
Mulder, Frans A. A.
author_facet Oktaviani, Nur Alia
Otten, Renee
Dijkstra, Klaas
Scheek, Ruud M.
Thulin, Eva
Akke, Mikael
Mulder, Frans A. A.
author_sort Oktaviani, Nur Alia
collection PubMed
description Here we present the 100% complete assignment chemical shift of non-labile (1)H, (15)N and (13)C nuclei of Calbindin D(9k) P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysNζ as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Calbindin D(9k) extend beyond previous studies reaching near-completeness (Vis et al. in Biochem 33:14858–14870, 1994; Yamazaki et al. in J Am Chem Soc 116:6464–6465, 1994; Yamazaki et al. in Biochem 32:5656–5669, 1993b).
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spelling pubmed-30492232011-04-05 100% complete assignment of non-labile (1)H, (13)C, and (15)N signals for calcium-loaded calbindin D(9k) P43G Oktaviani, Nur Alia Otten, Renee Dijkstra, Klaas Scheek, Ruud M. Thulin, Eva Akke, Mikael Mulder, Frans A. A. Biomol NMR Assign Article Here we present the 100% complete assignment chemical shift of non-labile (1)H, (15)N and (13)C nuclei of Calbindin D(9k) P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysNζ as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Calbindin D(9k) extend beyond previous studies reaching near-completeness (Vis et al. in Biochem 33:14858–14870, 1994; Yamazaki et al. in J Am Chem Soc 116:6464–6465, 1994; Yamazaki et al. in Biochem 32:5656–5669, 1993b). Springer Netherlands 2010-11-12 2011 /pmc/articles/PMC3049223/ /pubmed/21069485 http://dx.doi.org/10.1007/s12104-010-9272-3 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Oktaviani, Nur Alia
Otten, Renee
Dijkstra, Klaas
Scheek, Ruud M.
Thulin, Eva
Akke, Mikael
Mulder, Frans A. A.
100% complete assignment of non-labile (1)H, (13)C, and (15)N signals for calcium-loaded calbindin D(9k) P43G
title 100% complete assignment of non-labile (1)H, (13)C, and (15)N signals for calcium-loaded calbindin D(9k) P43G
title_full 100% complete assignment of non-labile (1)H, (13)C, and (15)N signals for calcium-loaded calbindin D(9k) P43G
title_fullStr 100% complete assignment of non-labile (1)H, (13)C, and (15)N signals for calcium-loaded calbindin D(9k) P43G
title_full_unstemmed 100% complete assignment of non-labile (1)H, (13)C, and (15)N signals for calcium-loaded calbindin D(9k) P43G
title_short 100% complete assignment of non-labile (1)H, (13)C, and (15)N signals for calcium-loaded calbindin D(9k) P43G
title_sort 100% complete assignment of non-labile (1)h, (13)c, and (15)n signals for calcium-loaded calbindin d(9k) p43g
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3049223/
https://www.ncbi.nlm.nih.gov/pubmed/21069485
http://dx.doi.org/10.1007/s12104-010-9272-3
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