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Survey of the Phosphorylation Status of the Schizosaccharomyces pombe Deubiquitinating Enzyme (DUB) Family

[Image: see text] Ubiquitination plays a role in virtually every cellular signaling pathway ranging from cell cycle control to DNA damage response to endocytosis and gene regulation. The bulk of our knowledge of the ubiquitination system is centered on modification of specific substrate proteins and...

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Autores principales: McLean, Janel R., Kouranti, Ilektra, Gould, Kathleen L.
Formato: Texto
Lenguaje:English
Publicado: American Chemical Society 2010
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3049645/
https://www.ncbi.nlm.nih.gov/pubmed/21182284
http://dx.doi.org/10.1021/pr100985s
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author McLean, Janel R.
Kouranti, Ilektra
Gould, Kathleen L.
author_facet McLean, Janel R.
Kouranti, Ilektra
Gould, Kathleen L.
author_sort McLean, Janel R.
collection PubMed
description [Image: see text] Ubiquitination plays a role in virtually every cellular signaling pathway ranging from cell cycle control to DNA damage response to endocytosis and gene regulation. The bulk of our knowledge of the ubiquitination system is centered on modification of specific substrate proteins and the enzymatic cascade of ubiquitination. Our understanding of the regulation of the reversal of these modifications (deubiquitination) lags significantly behind. We recently reported a multifaceted study of the fission yeast Schizosaccharomyces pombe DUBs including characterization of their binding partners, in vitro enzymatic activity and subcellular localization.(1) Over half of the 20 fission yeast DUBs have a stable protein partner and some of those partners regulate the localization and/or activity of their cognate DUB. As a next step in understanding how DUBs might otherwise be regulated, we investigated the phosphostatus of the entire fission yeast DUB family using LC−MS/MS, and here we discuss the possible implications of phosphoregulation.
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spelling pubmed-30496452011-03-08 Survey of the Phosphorylation Status of the Schizosaccharomyces pombe Deubiquitinating Enzyme (DUB) Family McLean, Janel R. Kouranti, Ilektra Gould, Kathleen L. J Proteome Res [Image: see text] Ubiquitination plays a role in virtually every cellular signaling pathway ranging from cell cycle control to DNA damage response to endocytosis and gene regulation. The bulk of our knowledge of the ubiquitination system is centered on modification of specific substrate proteins and the enzymatic cascade of ubiquitination. Our understanding of the regulation of the reversal of these modifications (deubiquitination) lags significantly behind. We recently reported a multifaceted study of the fission yeast Schizosaccharomyces pombe DUBs including characterization of their binding partners, in vitro enzymatic activity and subcellular localization.(1) Over half of the 20 fission yeast DUBs have a stable protein partner and some of those partners regulate the localization and/or activity of their cognate DUB. As a next step in understanding how DUBs might otherwise be regulated, we investigated the phosphostatus of the entire fission yeast DUB family using LC−MS/MS, and here we discuss the possible implications of phosphoregulation. American Chemical Society 2010-12-23 2011-03-04 /pmc/articles/PMC3049645/ /pubmed/21182284 http://dx.doi.org/10.1021/pr100985s Text en Copyright © 2010 American Chemical Society http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.
spellingShingle McLean, Janel R.
Kouranti, Ilektra
Gould, Kathleen L.
Survey of the Phosphorylation Status of the Schizosaccharomyces pombe Deubiquitinating Enzyme (DUB) Family
title Survey of the Phosphorylation Status of the Schizosaccharomyces pombe Deubiquitinating Enzyme (DUB) Family
title_full Survey of the Phosphorylation Status of the Schizosaccharomyces pombe Deubiquitinating Enzyme (DUB) Family
title_fullStr Survey of the Phosphorylation Status of the Schizosaccharomyces pombe Deubiquitinating Enzyme (DUB) Family
title_full_unstemmed Survey of the Phosphorylation Status of the Schizosaccharomyces pombe Deubiquitinating Enzyme (DUB) Family
title_short Survey of the Phosphorylation Status of the Schizosaccharomyces pombe Deubiquitinating Enzyme (DUB) Family
title_sort survey of the phosphorylation status of the schizosaccharomyces pombe deubiquitinating enzyme (dub) family
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3049645/
https://www.ncbi.nlm.nih.gov/pubmed/21182284
http://dx.doi.org/10.1021/pr100985s
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