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Expression and Localization of CLC Chloride Transport Proteins in the Avian Retina

Members of the ubiquitously expressed CLC protein family of chloride channels and transporters play important roles in regulating cellular chloride and pH. The CLCs that function as Cl(−)/H(+) antiporters, ClCs 3–7, are essential in particular for the acidification of endosomal compartments and prot...

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Detalles Bibliográficos
Autores principales: McMains, Emily, Krishnan, Vijai, Prasad, Sujitha, Gleason, Evanna
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3049779/
https://www.ncbi.nlm.nih.gov/pubmed/21408174
http://dx.doi.org/10.1371/journal.pone.0017647
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author McMains, Emily
Krishnan, Vijai
Prasad, Sujitha
Gleason, Evanna
author_facet McMains, Emily
Krishnan, Vijai
Prasad, Sujitha
Gleason, Evanna
author_sort McMains, Emily
collection PubMed
description Members of the ubiquitously expressed CLC protein family of chloride channels and transporters play important roles in regulating cellular chloride and pH. The CLCs that function as Cl(−)/H(+) antiporters, ClCs 3–7, are essential in particular for the acidification of endosomal compartments and protein degradation. These proteins are broadly expressed in the nervous system, and mutations that disrupt their expression are responsible for several human genetic diseases. Furthermore, knock-out of ClC3 and ClC7 in the mouse result in the degeneration of the hippocampus and the retina. Despite this evidence of their importance in retinal function, the expression patterns of different CLC transporters in different retinal cell types are as yet undescribed. Previous work in our lab has shown that in chicken amacrine cells, internal Cl(−) can be dynamic. To determine whether CLCs have the potential to participate, we used PCR and immunohistochemical techniques to examine CLC transporter expression in the chicken retina. We observed a high level of variation in the retinal expression levels and patterns among the different CLC proteins examined. These findings, which represent the first systematic investigation of CLC transporter expression in the retina, support diverse functions for the different CLCs in this tissue.
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spelling pubmed-30497792011-03-15 Expression and Localization of CLC Chloride Transport Proteins in the Avian Retina McMains, Emily Krishnan, Vijai Prasad, Sujitha Gleason, Evanna PLoS One Research Article Members of the ubiquitously expressed CLC protein family of chloride channels and transporters play important roles in regulating cellular chloride and pH. The CLCs that function as Cl(−)/H(+) antiporters, ClCs 3–7, are essential in particular for the acidification of endosomal compartments and protein degradation. These proteins are broadly expressed in the nervous system, and mutations that disrupt their expression are responsible for several human genetic diseases. Furthermore, knock-out of ClC3 and ClC7 in the mouse result in the degeneration of the hippocampus and the retina. Despite this evidence of their importance in retinal function, the expression patterns of different CLC transporters in different retinal cell types are as yet undescribed. Previous work in our lab has shown that in chicken amacrine cells, internal Cl(−) can be dynamic. To determine whether CLCs have the potential to participate, we used PCR and immunohistochemical techniques to examine CLC transporter expression in the chicken retina. We observed a high level of variation in the retinal expression levels and patterns among the different CLC proteins examined. These findings, which represent the first systematic investigation of CLC transporter expression in the retina, support diverse functions for the different CLCs in this tissue. Public Library of Science 2011-03-07 /pmc/articles/PMC3049779/ /pubmed/21408174 http://dx.doi.org/10.1371/journal.pone.0017647 Text en McMains et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
McMains, Emily
Krishnan, Vijai
Prasad, Sujitha
Gleason, Evanna
Expression and Localization of CLC Chloride Transport Proteins in the Avian Retina
title Expression and Localization of CLC Chloride Transport Proteins in the Avian Retina
title_full Expression and Localization of CLC Chloride Transport Proteins in the Avian Retina
title_fullStr Expression and Localization of CLC Chloride Transport Proteins in the Avian Retina
title_full_unstemmed Expression and Localization of CLC Chloride Transport Proteins in the Avian Retina
title_short Expression and Localization of CLC Chloride Transport Proteins in the Avian Retina
title_sort expression and localization of clc chloride transport proteins in the avian retina
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3049779/
https://www.ncbi.nlm.nih.gov/pubmed/21408174
http://dx.doi.org/10.1371/journal.pone.0017647
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