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Adrenoceptor stimulation does not affect ICAM-1 and VCAM-1 expression in vitro

BACKGROUND: Endothelial adhesion molecules ICAM-1 (CD54) and VCAM-1 (CD106) mediate cellular adhesion and transcellular migration. Cell adhesion and diapedesis have a key role in the course of shock and sepsis. During severe sepsis, adrenoceptor agonist levels may be increased due to endogenous prod...

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Autores principales: Ruschulte, Heiner, Scheinichen, Dirk, van Griensven, Martijn, Weyrauch, Susanne, Liefing, Wibke, Harrmeijer, Birgitt, Przemeck, Michael, Jüttner, Björn
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3050730/
https://www.ncbi.nlm.nih.gov/pubmed/21352518
http://dx.doi.org/10.1186/1756-0500-4-40
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author Ruschulte, Heiner
Scheinichen, Dirk
van Griensven, Martijn
Weyrauch, Susanne
Liefing, Wibke
Harrmeijer, Birgitt
Przemeck, Michael
Jüttner, Björn
author_facet Ruschulte, Heiner
Scheinichen, Dirk
van Griensven, Martijn
Weyrauch, Susanne
Liefing, Wibke
Harrmeijer, Birgitt
Przemeck, Michael
Jüttner, Björn
author_sort Ruschulte, Heiner
collection PubMed
description BACKGROUND: Endothelial adhesion molecules ICAM-1 (CD54) and VCAM-1 (CD106) mediate cellular adhesion and transcellular migration. Cell adhesion and diapedesis have a key role in the course of shock and sepsis. During severe sepsis, adrenoceptor agonist levels may be increased due to endogenous production or due to intensive care treatment. As yet, the influence of β1 or β2 agonists on adhesion molecule formation on endothelial cells has remained unclear. METHODS: Cultured human umbilical vein endothelial cells were stimulated with E. coli. Following bacterial stimulation the cells were incubated with either β2 receptor agonist terbutaline or β1 agonist norepinephrine. ICAM-1 and VCAM-1 expression were examined using flow cytometry. RESULTS: Administration of norepinephrine did not cause increases of both CD54 and CD106 in stimulated HUVEC. Compared to negative controls the bacterial stimulation itself led to an increase of adhesion molecules. Following administration of terbutaline no significant increase in CD54 expression was found. CONCLUSIONS: Bacterial stimulation led to an increase of adhesion molecule expression. Adrenoceptor stimulation of activated endothelial cells did not cause significant increases of cellular adhesion molecules.
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spelling pubmed-30507302011-03-09 Adrenoceptor stimulation does not affect ICAM-1 and VCAM-1 expression in vitro Ruschulte, Heiner Scheinichen, Dirk van Griensven, Martijn Weyrauch, Susanne Liefing, Wibke Harrmeijer, Birgitt Przemeck, Michael Jüttner, Björn BMC Res Notes Research Article BACKGROUND: Endothelial adhesion molecules ICAM-1 (CD54) and VCAM-1 (CD106) mediate cellular adhesion and transcellular migration. Cell adhesion and diapedesis have a key role in the course of shock and sepsis. During severe sepsis, adrenoceptor agonist levels may be increased due to endogenous production or due to intensive care treatment. As yet, the influence of β1 or β2 agonists on adhesion molecule formation on endothelial cells has remained unclear. METHODS: Cultured human umbilical vein endothelial cells were stimulated with E. coli. Following bacterial stimulation the cells were incubated with either β2 receptor agonist terbutaline or β1 agonist norepinephrine. ICAM-1 and VCAM-1 expression were examined using flow cytometry. RESULTS: Administration of norepinephrine did not cause increases of both CD54 and CD106 in stimulated HUVEC. Compared to negative controls the bacterial stimulation itself led to an increase of adhesion molecules. Following administration of terbutaline no significant increase in CD54 expression was found. CONCLUSIONS: Bacterial stimulation led to an increase of adhesion molecule expression. Adrenoceptor stimulation of activated endothelial cells did not cause significant increases of cellular adhesion molecules. BioMed Central 2011-02-25 /pmc/articles/PMC3050730/ /pubmed/21352518 http://dx.doi.org/10.1186/1756-0500-4-40 Text en Copyright ©2011 Ruschulte et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ruschulte, Heiner
Scheinichen, Dirk
van Griensven, Martijn
Weyrauch, Susanne
Liefing, Wibke
Harrmeijer, Birgitt
Przemeck, Michael
Jüttner, Björn
Adrenoceptor stimulation does not affect ICAM-1 and VCAM-1 expression in vitro
title Adrenoceptor stimulation does not affect ICAM-1 and VCAM-1 expression in vitro
title_full Adrenoceptor stimulation does not affect ICAM-1 and VCAM-1 expression in vitro
title_fullStr Adrenoceptor stimulation does not affect ICAM-1 and VCAM-1 expression in vitro
title_full_unstemmed Adrenoceptor stimulation does not affect ICAM-1 and VCAM-1 expression in vitro
title_short Adrenoceptor stimulation does not affect ICAM-1 and VCAM-1 expression in vitro
title_sort adrenoceptor stimulation does not affect icam-1 and vcam-1 expression in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3050730/
https://www.ncbi.nlm.nih.gov/pubmed/21352518
http://dx.doi.org/10.1186/1756-0500-4-40
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