Effects of ulinastatin and docataxel on breast tumor growth and expression of IL-6, IL-8, and TNF-α
OBJECTIVE: This study investigated the effects of Ulinastatin (UTI) and docataxel (Taxotere, TAX) on tumor growth and expression of interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) in breast cancer. METHODS: MDA-MB-231 human breast carcinoma cells were cultured in vitr...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3050767/ https://www.ncbi.nlm.nih.gov/pubmed/21345194 http://dx.doi.org/10.1186/1756-9966-30-22 |
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author | Zhao, Xiaoliang Sun, Xin Gao, Feng Luo, Jie Sun, Zhijun |
author_facet | Zhao, Xiaoliang Sun, Xin Gao, Feng Luo, Jie Sun, Zhijun |
author_sort | Zhao, Xiaoliang |
collection | PubMed |
description | OBJECTIVE: This study investigated the effects of Ulinastatin (UTI) and docataxel (Taxotere, TAX) on tumor growth and expression of interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) in breast cancer. METHODS: MDA-MB-231 human breast carcinoma cells were cultured in vitro and injected into nude mice to establish breast tumor xenografts in vivo. Cultured cells and mice with tumors were randomly divided into four groups for treatment with TAX, UTI, and TAX+UTI. The effects of these drug treatments on cell proliferation and apoptosis was measured using the MTT assay and the Annexin V/propidium iodide (PI) double-staining method, respectively. IL-6, IL-8, and TNF-α expression levels were determined by measuring mRNA transcripts in cultured cells by RT-PCR and cytokine proteins in solid tumors using immunohistochemistry. RESULTS: UTI, TAX, and UTI+TAX inhibited the growth of MDA-MB-231 cells in vitro and tumors in vivo. These two drugs, particularly when used in combination, promote tumor cell apoptosis and down-regulate the expression IL-6, IL-8, and TNF-α cytokines. CONCLUSION: Both UTI and TAX inhibited the growth of MDA-MB-231 breast carcinoma cells. UTI enhanced the inhibitory effect of TAX by a mechanism consistent with the down-regulated expression of IL-6, IL-8, and TNF-α. |
format | Text |
id | pubmed-3050767 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30507672011-04-06 Effects of ulinastatin and docataxel on breast tumor growth and expression of IL-6, IL-8, and TNF-α Zhao, Xiaoliang Sun, Xin Gao, Feng Luo, Jie Sun, Zhijun J Exp Clin Cancer Res Research OBJECTIVE: This study investigated the effects of Ulinastatin (UTI) and docataxel (Taxotere, TAX) on tumor growth and expression of interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) in breast cancer. METHODS: MDA-MB-231 human breast carcinoma cells were cultured in vitro and injected into nude mice to establish breast tumor xenografts in vivo. Cultured cells and mice with tumors were randomly divided into four groups for treatment with TAX, UTI, and TAX+UTI. The effects of these drug treatments on cell proliferation and apoptosis was measured using the MTT assay and the Annexin V/propidium iodide (PI) double-staining method, respectively. IL-6, IL-8, and TNF-α expression levels were determined by measuring mRNA transcripts in cultured cells by RT-PCR and cytokine proteins in solid tumors using immunohistochemistry. RESULTS: UTI, TAX, and UTI+TAX inhibited the growth of MDA-MB-231 cells in vitro and tumors in vivo. These two drugs, particularly when used in combination, promote tumor cell apoptosis and down-regulate the expression IL-6, IL-8, and TNF-α cytokines. CONCLUSION: Both UTI and TAX inhibited the growth of MDA-MB-231 breast carcinoma cells. UTI enhanced the inhibitory effect of TAX by a mechanism consistent with the down-regulated expression of IL-6, IL-8, and TNF-α. BioMed Central 2011-02-23 /pmc/articles/PMC3050767/ /pubmed/21345194 http://dx.doi.org/10.1186/1756-9966-30-22 Text en Copyright ©2011 Zhao et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Zhao, Xiaoliang Sun, Xin Gao, Feng Luo, Jie Sun, Zhijun Effects of ulinastatin and docataxel on breast tumor growth and expression of IL-6, IL-8, and TNF-α |
title | Effects of ulinastatin and docataxel on breast tumor growth and expression of IL-6, IL-8, and TNF-α |
title_full | Effects of ulinastatin and docataxel on breast tumor growth and expression of IL-6, IL-8, and TNF-α |
title_fullStr | Effects of ulinastatin and docataxel on breast tumor growth and expression of IL-6, IL-8, and TNF-α |
title_full_unstemmed | Effects of ulinastatin and docataxel on breast tumor growth and expression of IL-6, IL-8, and TNF-α |
title_short | Effects of ulinastatin and docataxel on breast tumor growth and expression of IL-6, IL-8, and TNF-α |
title_sort | effects of ulinastatin and docataxel on breast tumor growth and expression of il-6, il-8, and tnf-α |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3050767/ https://www.ncbi.nlm.nih.gov/pubmed/21345194 http://dx.doi.org/10.1186/1756-9966-30-22 |
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