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Nuclear Mitochondrial DNA Activates Replication in Saccharomyces cerevisiae

The nuclear genome of eukaryotes is colonized by DNA fragments of mitochondrial origin, called NUMTs. These insertions have been associated with a variety of germ-line diseases in humans. The significance of this uptake of potentially dangerous sequences into the nuclear genome is unclear. Here we p...

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Detalles Bibliográficos
Autores principales: Chatre, Laurent, Ricchetti, Miria
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3050842/
https://www.ncbi.nlm.nih.gov/pubmed/21408151
http://dx.doi.org/10.1371/journal.pone.0017235
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author Chatre, Laurent
Ricchetti, Miria
author_facet Chatre, Laurent
Ricchetti, Miria
author_sort Chatre, Laurent
collection PubMed
description The nuclear genome of eukaryotes is colonized by DNA fragments of mitochondrial origin, called NUMTs. These insertions have been associated with a variety of germ-line diseases in humans. The significance of this uptake of potentially dangerous sequences into the nuclear genome is unclear. Here we provide functional evidence that sequences of mitochondrial origin promote nuclear DNA replication in Saccharomyces cerevisiae. We show that NUMTs are rich in key autonomously replicating sequence (ARS) consensus motifs, whose mutation results in the reduction or loss of DNA replication activity. Furthermore, 2D-gel analysis of the mrc1 mutant exposed to hydroxyurea shows that several NUMTs function as late chromosomal origins. We also show that NUMTs located close to or within ARS provide key sequence elements for replication. Thus NUMTs can act as independent origins, when inserted in an appropriate genomic context or affect the efficiency of pre-existing origins. These findings show that migratory mitochondrial DNAs can impact on the replication of the nuclear region they are inserted in.
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spelling pubmed-30508422011-03-15 Nuclear Mitochondrial DNA Activates Replication in Saccharomyces cerevisiae Chatre, Laurent Ricchetti, Miria PLoS One Research Article The nuclear genome of eukaryotes is colonized by DNA fragments of mitochondrial origin, called NUMTs. These insertions have been associated with a variety of germ-line diseases in humans. The significance of this uptake of potentially dangerous sequences into the nuclear genome is unclear. Here we provide functional evidence that sequences of mitochondrial origin promote nuclear DNA replication in Saccharomyces cerevisiae. We show that NUMTs are rich in key autonomously replicating sequence (ARS) consensus motifs, whose mutation results in the reduction or loss of DNA replication activity. Furthermore, 2D-gel analysis of the mrc1 mutant exposed to hydroxyurea shows that several NUMTs function as late chromosomal origins. We also show that NUMTs located close to or within ARS provide key sequence elements for replication. Thus NUMTs can act as independent origins, when inserted in an appropriate genomic context or affect the efficiency of pre-existing origins. These findings show that migratory mitochondrial DNAs can impact on the replication of the nuclear region they are inserted in. Public Library of Science 2011-03-08 /pmc/articles/PMC3050842/ /pubmed/21408151 http://dx.doi.org/10.1371/journal.pone.0017235 Text en Chatre, Ricchetti. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chatre, Laurent
Ricchetti, Miria
Nuclear Mitochondrial DNA Activates Replication in Saccharomyces cerevisiae
title Nuclear Mitochondrial DNA Activates Replication in Saccharomyces cerevisiae
title_full Nuclear Mitochondrial DNA Activates Replication in Saccharomyces cerevisiae
title_fullStr Nuclear Mitochondrial DNA Activates Replication in Saccharomyces cerevisiae
title_full_unstemmed Nuclear Mitochondrial DNA Activates Replication in Saccharomyces cerevisiae
title_short Nuclear Mitochondrial DNA Activates Replication in Saccharomyces cerevisiae
title_sort nuclear mitochondrial dna activates replication in saccharomyces cerevisiae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3050842/
https://www.ncbi.nlm.nih.gov/pubmed/21408151
http://dx.doi.org/10.1371/journal.pone.0017235
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