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Outer Membrane Vesicles as a Candidate Vaccine against Edwardsiellosis
Infection with Edwardsiella tarda, a Gram-negative bacterium, causes high morbidity and mortality in both marine and freshwater fish. Outer membrane vesicles (OMVs) released from Gram-negative bacteria are known to play important roles in bacterial pathogenesis and host immune responses, but no such...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3050902/ https://www.ncbi.nlm.nih.gov/pubmed/21408115 http://dx.doi.org/10.1371/journal.pone.0017629 |
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author | Park, Seong Bin Jang, Ho Bin Nho, Seong Won Cha, In Seok Hikima, Jun-ichi Ohtani, Maki Aoki, Takashi Jung, Tae Sung |
author_facet | Park, Seong Bin Jang, Ho Bin Nho, Seong Won Cha, In Seok Hikima, Jun-ichi Ohtani, Maki Aoki, Takashi Jung, Tae Sung |
author_sort | Park, Seong Bin |
collection | PubMed |
description | Infection with Edwardsiella tarda, a Gram-negative bacterium, causes high morbidity and mortality in both marine and freshwater fish. Outer membrane vesicles (OMVs) released from Gram-negative bacteria are known to play important roles in bacterial pathogenesis and host immune responses, but no such roles for E. tarda OMVs have yet been described. In the present study, we investigated the proteomic composition of OMVs and the immunostimulatory effect of OMVs in a natural host, as well as the efficacy of OMVs when used as a vaccine against E. tarda infection. A total of 74 proteins, from diverse subcellular fractions, were identified in OMVs. These included a variety of important virulence factors, such as hemolysin, OmpA, porin, GAPDH, EseB, EseC, EseD, EvpC, EvpP, lipoprotein, flagellin, and fimbrial protein. When OMVs were administrated to olive flounder, significant induction of mRNAs encoding IL-1β, IL-6, TNFα, and IFNγ was observed, compared with the levels seen in fish injected with formalin-killed E. tarda. In a vaccine trial, olive flounder given OMVs were more effectively protected (p<0.0001) than were control fish. Investigation of OMVs may be useful not only for understanding the pathogenesis of E. tarda but also in development of an effective vaccine against edwardsiellosis. |
format | Text |
id | pubmed-3050902 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30509022011-03-15 Outer Membrane Vesicles as a Candidate Vaccine against Edwardsiellosis Park, Seong Bin Jang, Ho Bin Nho, Seong Won Cha, In Seok Hikima, Jun-ichi Ohtani, Maki Aoki, Takashi Jung, Tae Sung PLoS One Research Article Infection with Edwardsiella tarda, a Gram-negative bacterium, causes high morbidity and mortality in both marine and freshwater fish. Outer membrane vesicles (OMVs) released from Gram-negative bacteria are known to play important roles in bacterial pathogenesis and host immune responses, but no such roles for E. tarda OMVs have yet been described. In the present study, we investigated the proteomic composition of OMVs and the immunostimulatory effect of OMVs in a natural host, as well as the efficacy of OMVs when used as a vaccine against E. tarda infection. A total of 74 proteins, from diverse subcellular fractions, were identified in OMVs. These included a variety of important virulence factors, such as hemolysin, OmpA, porin, GAPDH, EseB, EseC, EseD, EvpC, EvpP, lipoprotein, flagellin, and fimbrial protein. When OMVs were administrated to olive flounder, significant induction of mRNAs encoding IL-1β, IL-6, TNFα, and IFNγ was observed, compared with the levels seen in fish injected with formalin-killed E. tarda. In a vaccine trial, olive flounder given OMVs were more effectively protected (p<0.0001) than were control fish. Investigation of OMVs may be useful not only for understanding the pathogenesis of E. tarda but also in development of an effective vaccine against edwardsiellosis. Public Library of Science 2011-03-08 /pmc/articles/PMC3050902/ /pubmed/21408115 http://dx.doi.org/10.1371/journal.pone.0017629 Text en Park et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Park, Seong Bin Jang, Ho Bin Nho, Seong Won Cha, In Seok Hikima, Jun-ichi Ohtani, Maki Aoki, Takashi Jung, Tae Sung Outer Membrane Vesicles as a Candidate Vaccine against Edwardsiellosis |
title | Outer Membrane Vesicles as a Candidate Vaccine against Edwardsiellosis |
title_full | Outer Membrane Vesicles as a Candidate Vaccine against Edwardsiellosis |
title_fullStr | Outer Membrane Vesicles as a Candidate Vaccine against Edwardsiellosis |
title_full_unstemmed | Outer Membrane Vesicles as a Candidate Vaccine against Edwardsiellosis |
title_short | Outer Membrane Vesicles as a Candidate Vaccine against Edwardsiellosis |
title_sort | outer membrane vesicles as a candidate vaccine against edwardsiellosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3050902/ https://www.ncbi.nlm.nih.gov/pubmed/21408115 http://dx.doi.org/10.1371/journal.pone.0017629 |
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