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Comparison of Diagnostic Performance of Three Real-Time PCR Kits for Detecting Mycobacterium Species
PURPOSE: PCR is widely used for rapidly and accurately detecting Mycobacterium Species. The purpose of this study was to assess the diagnostic performance of three real-time PCR kits and evaluate the concordance with two older PCR methods. MATERIALS AND METHODS: Using 128 samples, the five PCR metho...
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Formato: | Texto |
Lenguaje: | English |
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Yonsei University College of Medicine
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3051208/ https://www.ncbi.nlm.nih.gov/pubmed/21319350 http://dx.doi.org/10.3349/ymj.2011.52.2.301 |
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author | Cho, Sun Young Kim, Min Jin Suh, Jin-Tae Lee, Hee Joo |
author_facet | Cho, Sun Young Kim, Min Jin Suh, Jin-Tae Lee, Hee Joo |
author_sort | Cho, Sun Young |
collection | PubMed |
description | PURPOSE: PCR is widely used for rapidly and accurately detecting Mycobacterium Species. The purpose of this study was to assess the diagnostic performance of three real-time PCR kits and evaluate the concordance with two older PCR methods. MATERIALS AND METHODS: Using 128 samples, the five PCR methods were assessed, including an in-house PCR protocol, the COBAS Amplicor MTB, the COBAS TaqMan MTB, the AdvanSure TB/NTM real-time PCR, and the Real-Q M. tuberculosis kit. The discrepant results were further examined by DNA sequencing and using the AdvanSure Mycobacteria Genotyping Chip for complete analysis. RESULTS: For Mycobacterium tuberculosis (MTB) detection, all five kits showed 100% matching results (positive; N = 11 and negative; N = 80). In non-tuberculous mycobacterium (NTM) discrimination, the AdvanSure yielded two true-positive outcomes from M. intracellulare and one false positive outcome, while the Real-Q resulted in one true-positive outcome and one false negative outcome for each case and another false negative result using the provided DNA samples. CONCLUSION: Real-time PCR, yielded results that were comparable to those of the older PCR methods for detecting MTB. However, there were disagreements among the applied kits in regard to the sample test results for detecting NTM. Therefore, we recommend that additional confirmatory measures such as DNA sequencing should be implemented in such cases, and further research with using a larger numbers of samples is warranted to improve the detection of NTM. |
format | Text |
id | pubmed-3051208 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Yonsei University College of Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-30512082011-03-09 Comparison of Diagnostic Performance of Three Real-Time PCR Kits for Detecting Mycobacterium Species Cho, Sun Young Kim, Min Jin Suh, Jin-Tae Lee, Hee Joo Yonsei Med J Original Article PURPOSE: PCR is widely used for rapidly and accurately detecting Mycobacterium Species. The purpose of this study was to assess the diagnostic performance of three real-time PCR kits and evaluate the concordance with two older PCR methods. MATERIALS AND METHODS: Using 128 samples, the five PCR methods were assessed, including an in-house PCR protocol, the COBAS Amplicor MTB, the COBAS TaqMan MTB, the AdvanSure TB/NTM real-time PCR, and the Real-Q M. tuberculosis kit. The discrepant results were further examined by DNA sequencing and using the AdvanSure Mycobacteria Genotyping Chip for complete analysis. RESULTS: For Mycobacterium tuberculosis (MTB) detection, all five kits showed 100% matching results (positive; N = 11 and negative; N = 80). In non-tuberculous mycobacterium (NTM) discrimination, the AdvanSure yielded two true-positive outcomes from M. intracellulare and one false positive outcome, while the Real-Q resulted in one true-positive outcome and one false negative outcome for each case and another false negative result using the provided DNA samples. CONCLUSION: Real-time PCR, yielded results that were comparable to those of the older PCR methods for detecting MTB. However, there were disagreements among the applied kits in regard to the sample test results for detecting NTM. Therefore, we recommend that additional confirmatory measures such as DNA sequencing should be implemented in such cases, and further research with using a larger numbers of samples is warranted to improve the detection of NTM. Yonsei University College of Medicine 2011-03-01 2011-01-29 /pmc/articles/PMC3051208/ /pubmed/21319350 http://dx.doi.org/10.3349/ymj.2011.52.2.301 Text en © Copyright: Yonsei University College of Medicine 2011 http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Cho, Sun Young Kim, Min Jin Suh, Jin-Tae Lee, Hee Joo Comparison of Diagnostic Performance of Three Real-Time PCR Kits for Detecting Mycobacterium Species |
title | Comparison of Diagnostic Performance of Three Real-Time PCR Kits for Detecting Mycobacterium Species |
title_full | Comparison of Diagnostic Performance of Three Real-Time PCR Kits for Detecting Mycobacterium Species |
title_fullStr | Comparison of Diagnostic Performance of Three Real-Time PCR Kits for Detecting Mycobacterium Species |
title_full_unstemmed | Comparison of Diagnostic Performance of Three Real-Time PCR Kits for Detecting Mycobacterium Species |
title_short | Comparison of Diagnostic Performance of Three Real-Time PCR Kits for Detecting Mycobacterium Species |
title_sort | comparison of diagnostic performance of three real-time pcr kits for detecting mycobacterium species |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3051208/ https://www.ncbi.nlm.nih.gov/pubmed/21319350 http://dx.doi.org/10.3349/ymj.2011.52.2.301 |
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