Cargando…

Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis

Staphylococcus aureus is a major cause of mastitis in ruminants. In ewe mastitis, symptoms range from subclinical to gangrenous mastitis. S. aureus factors or host-factors contributing to the different outcomes are not completely elucidated. In this study, experimental mastitis was induced on primip...

Descripción completa

Detalles Bibliográficos
Autores principales: Le Maréchal, Caroline, Jardin, Julien, Jan, Gwenaël, Even, Sergine, Pulido, Coralie, Guibert, Jean-Michel, Hernandez, David, François, Patrice, Schrenzel, Jacques, Demon, Dieter, Meyer, Evelyne, Berkova, Nadia, Thiéry, Richard, Vautor, Eric, Le Loir, Yves
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3052181/
https://www.ncbi.nlm.nih.gov/pubmed/21324116
http://dx.doi.org/10.1186/1297-9716-42-35
_version_ 1782199646634901504
author Le Maréchal, Caroline
Jardin, Julien
Jan, Gwenaël
Even, Sergine
Pulido, Coralie
Guibert, Jean-Michel
Hernandez, David
François, Patrice
Schrenzel, Jacques
Demon, Dieter
Meyer, Evelyne
Berkova, Nadia
Thiéry, Richard
Vautor, Eric
Le Loir, Yves
author_facet Le Maréchal, Caroline
Jardin, Julien
Jan, Gwenaël
Even, Sergine
Pulido, Coralie
Guibert, Jean-Michel
Hernandez, David
François, Patrice
Schrenzel, Jacques
Demon, Dieter
Meyer, Evelyne
Berkova, Nadia
Thiéry, Richard
Vautor, Eric
Le Loir, Yves
author_sort Le Maréchal, Caroline
collection PubMed
description Staphylococcus aureus is a major cause of mastitis in ruminants. In ewe mastitis, symptoms range from subclinical to gangrenous mastitis. S. aureus factors or host-factors contributing to the different outcomes are not completely elucidated. In this study, experimental mastitis was induced on primiparous ewes using two S. aureus strains, isolated from gangrenous (strain O11) or subclinical (strain O46) mastitis. Strains induced drastically distinct clinical symptoms when tested in ewe and mice experimental mastitis. Notably, they reproduced mild (O46) or severe (O11) mastitis in ewes. Ewe sera were used to identify staphylococcal immunoreactive proteins commonly or differentially produced during infections of variable severity and to define core and accessory seroproteomes. Such SERological Proteome Analysis (SERPA) allowed the identification of 89 immunoreactive proteins, of which only 52 (58.4%) were previously identified as immunogenic proteins in other staphylococcal infections. Among the 89 proteins identified, 74 appear to constitute the core seroproteome. Among the 15 remaining proteins defining the accessory seroproteome, 12 were specific for strain O11, 3 were specific for O46. Distribution of one protein specific for each mastitis severity was investigated in ten other strains isolated from subclinical or clinical mastitis. We report here for the first time the identification of staphylococcal immunogenic proteins common or specific to S. aureus strains responsible for mild or severe mastitis. These findings open avenues in S. aureus mastitis studies as some of these proteins, expressed in vivo, are likely to account for the success of S. aureus as a pathogen of the ruminant mammary gland.
format Text
id pubmed-3052181
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-30521812011-03-10 Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis Le Maréchal, Caroline Jardin, Julien Jan, Gwenaël Even, Sergine Pulido, Coralie Guibert, Jean-Michel Hernandez, David François, Patrice Schrenzel, Jacques Demon, Dieter Meyer, Evelyne Berkova, Nadia Thiéry, Richard Vautor, Eric Le Loir, Yves Vet Res Research Staphylococcus aureus is a major cause of mastitis in ruminants. In ewe mastitis, symptoms range from subclinical to gangrenous mastitis. S. aureus factors or host-factors contributing to the different outcomes are not completely elucidated. In this study, experimental mastitis was induced on primiparous ewes using two S. aureus strains, isolated from gangrenous (strain O11) or subclinical (strain O46) mastitis. Strains induced drastically distinct clinical symptoms when tested in ewe and mice experimental mastitis. Notably, they reproduced mild (O46) or severe (O11) mastitis in ewes. Ewe sera were used to identify staphylococcal immunoreactive proteins commonly or differentially produced during infections of variable severity and to define core and accessory seroproteomes. Such SERological Proteome Analysis (SERPA) allowed the identification of 89 immunoreactive proteins, of which only 52 (58.4%) were previously identified as immunogenic proteins in other staphylococcal infections. Among the 89 proteins identified, 74 appear to constitute the core seroproteome. Among the 15 remaining proteins defining the accessory seroproteome, 12 were specific for strain O11, 3 were specific for O46. Distribution of one protein specific for each mastitis severity was investigated in ten other strains isolated from subclinical or clinical mastitis. We report here for the first time the identification of staphylococcal immunogenic proteins common or specific to S. aureus strains responsible for mild or severe mastitis. These findings open avenues in S. aureus mastitis studies as some of these proteins, expressed in vivo, are likely to account for the success of S. aureus as a pathogen of the ruminant mammary gland. BioMed Central 2011 2011-02-15 /pmc/articles/PMC3052181/ /pubmed/21324116 http://dx.doi.org/10.1186/1297-9716-42-35 Text en Copyright ©2011 Le Maréchal et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Le Maréchal, Caroline
Jardin, Julien
Jan, Gwenaël
Even, Sergine
Pulido, Coralie
Guibert, Jean-Michel
Hernandez, David
François, Patrice
Schrenzel, Jacques
Demon, Dieter
Meyer, Evelyne
Berkova, Nadia
Thiéry, Richard
Vautor, Eric
Le Loir, Yves
Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis
title Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis
title_full Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis
title_fullStr Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis
title_full_unstemmed Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis
title_short Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis
title_sort staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3052181/
https://www.ncbi.nlm.nih.gov/pubmed/21324116
http://dx.doi.org/10.1186/1297-9716-42-35
work_keys_str_mv AT lemarechalcaroline staphylococcusaureusseroproteomesdiscriminateruminantisolatescausingmildorseveremastitis
AT jardinjulien staphylococcusaureusseroproteomesdiscriminateruminantisolatescausingmildorseveremastitis
AT jangwenael staphylococcusaureusseroproteomesdiscriminateruminantisolatescausingmildorseveremastitis
AT evensergine staphylococcusaureusseroproteomesdiscriminateruminantisolatescausingmildorseveremastitis
AT pulidocoralie staphylococcusaureusseroproteomesdiscriminateruminantisolatescausingmildorseveremastitis
AT guibertjeanmichel staphylococcusaureusseroproteomesdiscriminateruminantisolatescausingmildorseveremastitis
AT hernandezdavid staphylococcusaureusseroproteomesdiscriminateruminantisolatescausingmildorseveremastitis
AT francoispatrice staphylococcusaureusseroproteomesdiscriminateruminantisolatescausingmildorseveremastitis
AT schrenzeljacques staphylococcusaureusseroproteomesdiscriminateruminantisolatescausingmildorseveremastitis
AT demondieter staphylococcusaureusseroproteomesdiscriminateruminantisolatescausingmildorseveremastitis
AT meyerevelyne staphylococcusaureusseroproteomesdiscriminateruminantisolatescausingmildorseveremastitis
AT berkovanadia staphylococcusaureusseroproteomesdiscriminateruminantisolatescausingmildorseveremastitis
AT thieryrichard staphylococcusaureusseroproteomesdiscriminateruminantisolatescausingmildorseveremastitis
AT vautoreric staphylococcusaureusseroproteomesdiscriminateruminantisolatescausingmildorseveremastitis
AT leloiryves staphylococcusaureusseroproteomesdiscriminateruminantisolatescausingmildorseveremastitis