Applicability of RNA standards for evaluating RT-qPCR assays and platforms

The availability of diverse RT-qPCR assay formats and technologies hinder comparability of data between platforms. Reference standards to facilitate platform evaluation and comparability are needed. We have explored using universal RNA standards for comparing the performance of a novel qPCR platform...

Descripción completa

Detalles Bibliográficos
Autores principales: Devonshire, Alison S, Elaswarapu, Ramnath, Foy, Carole A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3052187/
https://www.ncbi.nlm.nih.gov/pubmed/21332979
http://dx.doi.org/10.1186/1471-2164-12-118
_version_ 1782199648050479104
author Devonshire, Alison S
Elaswarapu, Ramnath
Foy, Carole A
author_facet Devonshire, Alison S
Elaswarapu, Ramnath
Foy, Carole A
author_sort Devonshire, Alison S
collection PubMed
description The availability of diverse RT-qPCR assay formats and technologies hinder comparability of data between platforms. Reference standards to facilitate platform evaluation and comparability are needed. We have explored using universal RNA standards for comparing the performance of a novel qPCR platform (Fluidigm(® )BioMark™) against the widely used ABI 7900HT system. Our results show that such standards may form part of a toolkit to evaluate the key performance characteristics of platforms.
format Text
id pubmed-3052187
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-30521872011-03-10 Applicability of RNA standards for evaluating RT-qPCR assays and platforms Devonshire, Alison S Elaswarapu, Ramnath Foy, Carole A BMC Genomics Methodology Article The availability of diverse RT-qPCR assay formats and technologies hinder comparability of data between platforms. Reference standards to facilitate platform evaluation and comparability are needed. We have explored using universal RNA standards for comparing the performance of a novel qPCR platform (Fluidigm(® )BioMark™) against the widely used ABI 7900HT system. Our results show that such standards may form part of a toolkit to evaluate the key performance characteristics of platforms. BioMed Central 2011-02-18 /pmc/articles/PMC3052187/ /pubmed/21332979 http://dx.doi.org/10.1186/1471-2164-12-118 Text en Copyright ©2011 Devonshire et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology Article
Devonshire, Alison S
Elaswarapu, Ramnath
Foy, Carole A
Applicability of RNA standards for evaluating RT-qPCR assays and platforms
title Applicability of RNA standards for evaluating RT-qPCR assays and platforms
title_full Applicability of RNA standards for evaluating RT-qPCR assays and platforms
title_fullStr Applicability of RNA standards for evaluating RT-qPCR assays and platforms
title_full_unstemmed Applicability of RNA standards for evaluating RT-qPCR assays and platforms
title_short Applicability of RNA standards for evaluating RT-qPCR assays and platforms
title_sort applicability of rna standards for evaluating rt-qpcr assays and platforms
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3052187/
https://www.ncbi.nlm.nih.gov/pubmed/21332979
http://dx.doi.org/10.1186/1471-2164-12-118
work_keys_str_mv AT devonshirealisons applicabilityofrnastandardsforevaluatingrtqpcrassaysandplatforms
AT elaswarapuramnath applicabilityofrnastandardsforevaluatingrtqpcrassaysandplatforms
AT foycarolea applicabilityofrnastandardsforevaluatingrtqpcrassaysandplatforms

Ejemplares similares