Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MS

BACKGROUND: Xuesaitong (XST) injection, consisting of total saponins from Panax notoginseng, was widely used for the treatment of cardio- and cerebro-vascular diseases in China. This study develops a simple and global quality evaluation method for the quality control of XST. METHODS: High performanc...

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Autores principales: Yao, Hong, Shi, Peiying, Shao, Qing, Fan, Xiaohui
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3052241/
https://www.ncbi.nlm.nih.gov/pubmed/21349173
http://dx.doi.org/10.1186/1749-8546-6-9
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author Yao, Hong
Shi, Peiying
Shao, Qing
Fan, Xiaohui
author_facet Yao, Hong
Shi, Peiying
Shao, Qing
Fan, Xiaohui
author_sort Yao, Hong
collection PubMed
description BACKGROUND: Xuesaitong (XST) injection, consisting of total saponins from Panax notoginseng, was widely used for the treatment of cardio- and cerebro-vascular diseases in China. This study develops a simple and global quality evaluation method for the quality control of XST. METHODS: High performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to identify and quantify the chromatographic fingerprints of the XST injection. Characteristic common peaks were identified using HPLC with photo diode array detection/electrospray ionization tandem mass spectrometry (HPLC-PDA/ESI-MS(n)). RESULTS: Representative fingerprints from ten batches of samples showed 27 'common saponins' all of which were identified and quantified using ten reference saponins. CONCLUSION: Chemical fingerprinting and quantitative analysis identified most of the common saponins for the quality control of P. notoginseng products such as the XST injection.
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spelling pubmed-30522412011-03-10 Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MS Yao, Hong Shi, Peiying Shao, Qing Fan, Xiaohui Chin Med Research BACKGROUND: Xuesaitong (XST) injection, consisting of total saponins from Panax notoginseng, was widely used for the treatment of cardio- and cerebro-vascular diseases in China. This study develops a simple and global quality evaluation method for the quality control of XST. METHODS: High performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to identify and quantify the chromatographic fingerprints of the XST injection. Characteristic common peaks were identified using HPLC with photo diode array detection/electrospray ionization tandem mass spectrometry (HPLC-PDA/ESI-MS(n)). RESULTS: Representative fingerprints from ten batches of samples showed 27 'common saponins' all of which were identified and quantified using ten reference saponins. CONCLUSION: Chemical fingerprinting and quantitative analysis identified most of the common saponins for the quality control of P. notoginseng products such as the XST injection. BioMed Central 2011-02-24 /pmc/articles/PMC3052241/ /pubmed/21349173 http://dx.doi.org/10.1186/1749-8546-6-9 Text en Copyright ©2011 Yao et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Yao, Hong
Shi, Peiying
Shao, Qing
Fan, Xiaohui
Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MS
title Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MS
title_full Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MS
title_fullStr Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MS
title_full_unstemmed Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MS
title_short Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MS
title_sort chemical fingerprinting and quantitative analysis of a panax notoginseng preparation using hplc-uv and hplc-ms
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3052241/
https://www.ncbi.nlm.nih.gov/pubmed/21349173
http://dx.doi.org/10.1186/1749-8546-6-9
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