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In vitro toxicity of melamine against Tetrahymena pyriformis cells
This study assessed the toxicity of melamine against the unicellular eukaryotic system of Tetrahymena (T.) pyriformis exposed to 0, 0.05, 0.25, 0.5, 2.5, and 5 mg/mL of melamine. Cell growth curves of different cultures, the half maximum inhibition concentration (IC(50)) value of melamine, and morph...
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Formato: | Texto |
Lenguaje: | English |
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The Korean Society of Veterinary Science
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3053464/ https://www.ncbi.nlm.nih.gov/pubmed/21368560 http://dx.doi.org/10.4142/jvs.2011.12.1.27 |
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author | Wang, Zhengfang Chen, Liben Al-Kasir, Rashad Han, Bo |
author_facet | Wang, Zhengfang Chen, Liben Al-Kasir, Rashad Han, Bo |
author_sort | Wang, Zhengfang |
collection | PubMed |
description | This study assessed the toxicity of melamine against the unicellular eukaryotic system of Tetrahymena (T.) pyriformis exposed to 0, 0.05, 0.25, 0.5, 2.5, and 5 mg/mL of melamine. Cell growth curves of different cultures, the half maximum inhibition concentration (IC(50)) value of melamine, and morphological changes in cells were obtained via optical and transmission electron microscopic observation. The effects of eleven melamine concentrations, 0.25, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 and 5 mg/mL, on protein expression levels of T. pyriformis were examined using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The results showed an obvious inhibitory effect of melamine on the growth of eukaryotic cells. Cell growth dynamics indicated that the IC(50) value of melamine on T. pyriformis was 0.82 mg/mL. The cellular morphology was also affected in a concentration-dependent manner, with characteristics of atrophy or cell damage developing in the presence of melamine. The relative contents of the top four main proteins corresponding to peak mass-to-charge ratios (m/z) of 4466, m/z 6455, m/z 6514, and m/z 7772 in the MALDI-TOF-MS spectra were all found to be closely correlated with the melamine concentrations. In conclusion, exposure of eukaryotic cells to melamine could inhibit cell growth, cause changes in cytomorphology and even disturb the expression of proteins in a concentration-dependent manner. The described method of examining four sensitive proteins affected by melamine was also proposed to be used in a preliminary study to identify protein biomarkers in T. pyriformis. |
format | Text |
id | pubmed-3053464 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30534642011-03-22 In vitro toxicity of melamine against Tetrahymena pyriformis cells Wang, Zhengfang Chen, Liben Al-Kasir, Rashad Han, Bo J Vet Sci Original Article This study assessed the toxicity of melamine against the unicellular eukaryotic system of Tetrahymena (T.) pyriformis exposed to 0, 0.05, 0.25, 0.5, 2.5, and 5 mg/mL of melamine. Cell growth curves of different cultures, the half maximum inhibition concentration (IC(50)) value of melamine, and morphological changes in cells were obtained via optical and transmission electron microscopic observation. The effects of eleven melamine concentrations, 0.25, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 and 5 mg/mL, on protein expression levels of T. pyriformis were examined using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The results showed an obvious inhibitory effect of melamine on the growth of eukaryotic cells. Cell growth dynamics indicated that the IC(50) value of melamine on T. pyriformis was 0.82 mg/mL. The cellular morphology was also affected in a concentration-dependent manner, with characteristics of atrophy or cell damage developing in the presence of melamine. The relative contents of the top four main proteins corresponding to peak mass-to-charge ratios (m/z) of 4466, m/z 6455, m/z 6514, and m/z 7772 in the MALDI-TOF-MS spectra were all found to be closely correlated with the melamine concentrations. In conclusion, exposure of eukaryotic cells to melamine could inhibit cell growth, cause changes in cytomorphology and even disturb the expression of proteins in a concentration-dependent manner. The described method of examining four sensitive proteins affected by melamine was also proposed to be used in a preliminary study to identify protein biomarkers in T. pyriformis. The Korean Society of Veterinary Science 2011-03 2011-03-05 /pmc/articles/PMC3053464/ /pubmed/21368560 http://dx.doi.org/10.4142/jvs.2011.12.1.27 Text en Copyright © 2011 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Wang, Zhengfang Chen, Liben Al-Kasir, Rashad Han, Bo In vitro toxicity of melamine against Tetrahymena pyriformis cells |
title | In vitro toxicity of melamine against Tetrahymena pyriformis cells |
title_full | In vitro toxicity of melamine against Tetrahymena pyriformis cells |
title_fullStr | In vitro toxicity of melamine against Tetrahymena pyriformis cells |
title_full_unstemmed | In vitro toxicity of melamine against Tetrahymena pyriformis cells |
title_short | In vitro toxicity of melamine against Tetrahymena pyriformis cells |
title_sort | in vitro toxicity of melamine against tetrahymena pyriformis cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3053464/ https://www.ncbi.nlm.nih.gov/pubmed/21368560 http://dx.doi.org/10.4142/jvs.2011.12.1.27 |
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