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Diversity of Microorganisms Isolated from the Soil Sample surround Chroogomphus rutilus in the Beijing Region

Artificially cultivating Chroogomphus rutilus is too inefficient to be commercially feasible. Furthermore, isolating C. rutilus mycelia in the wild is difficult. Thus, it is important to determine the natural habitat of its fruiting body. This study focused on the ecology of the C. rutilus habitat t...

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Autores principales: Wang, Peng, Liu, Yu, Yin, Yonggang, Jin, Haojie, Wang, Shouxian, Xu, Feng, Zhao, Shuang, Geng, Xiaoli
Formato: Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3053533/
https://www.ncbi.nlm.nih.gov/pubmed/21448282
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author Wang, Peng
Liu, Yu
Yin, Yonggang
Jin, Haojie
Wang, Shouxian
Xu, Feng
Zhao, Shuang
Geng, Xiaoli
author_facet Wang, Peng
Liu, Yu
Yin, Yonggang
Jin, Haojie
Wang, Shouxian
Xu, Feng
Zhao, Shuang
Geng, Xiaoli
author_sort Wang, Peng
collection PubMed
description Artificially cultivating Chroogomphus rutilus is too inefficient to be commercially feasible. Furthermore, isolating C. rutilus mycelia in the wild is difficult. Thus, it is important to determine the natural habitat of its fruiting body. This study focused on the ecology of the C. rutilus habitat to isolate and classify beneficial microorganisms that could affect its growth, which could be used in future research on artificial cultivation. In total, 342 isolates were isolated from soil samples collected around a C. rutilus colony in the Beijing region. Of these, 22 bacterial and 14 fungal isolates were selected for sequencing and phylogenetic analysis, based on their growth characteristics and colony morphology. Using 16S rRNA gene sequence analysis, the bacterial isolates were divided into two monophyletic clusters which had significant hits to the genera Bacillus and Pseudomonas, respectively. Using internal transcribed spacer (ITS) sequence analysis, fungal isolates were divided into four monophyletic clusters: Penicillium, Trichoderma, Mortierella, and Bionectria. Moreover, the phylogenetic diversity of these isolates was analysed. The results indicated that numerous microorganisms were present in C. rutilus habitat. This was the first reported examination of the microbiological ecology of C. rutilus.
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spelling pubmed-30535332011-03-29 Diversity of Microorganisms Isolated from the Soil Sample surround Chroogomphus rutilus in the Beijing Region Wang, Peng Liu, Yu Yin, Yonggang Jin, Haojie Wang, Shouxian Xu, Feng Zhao, Shuang Geng, Xiaoli Int J Biol Sci Research Paper Artificially cultivating Chroogomphus rutilus is too inefficient to be commercially feasible. Furthermore, isolating C. rutilus mycelia in the wild is difficult. Thus, it is important to determine the natural habitat of its fruiting body. This study focused on the ecology of the C. rutilus habitat to isolate and classify beneficial microorganisms that could affect its growth, which could be used in future research on artificial cultivation. In total, 342 isolates were isolated from soil samples collected around a C. rutilus colony in the Beijing region. Of these, 22 bacterial and 14 fungal isolates were selected for sequencing and phylogenetic analysis, based on their growth characteristics and colony morphology. Using 16S rRNA gene sequence analysis, the bacterial isolates were divided into two monophyletic clusters which had significant hits to the genera Bacillus and Pseudomonas, respectively. Using internal transcribed spacer (ITS) sequence analysis, fungal isolates were divided into four monophyletic clusters: Penicillium, Trichoderma, Mortierella, and Bionectria. Moreover, the phylogenetic diversity of these isolates was analysed. The results indicated that numerous microorganisms were present in C. rutilus habitat. This was the first reported examination of the microbiological ecology of C. rutilus. Ivyspring International Publisher 2011-03-02 /pmc/articles/PMC3053533/ /pubmed/21448282 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Wang, Peng
Liu, Yu
Yin, Yonggang
Jin, Haojie
Wang, Shouxian
Xu, Feng
Zhao, Shuang
Geng, Xiaoli
Diversity of Microorganisms Isolated from the Soil Sample surround Chroogomphus rutilus in the Beijing Region
title Diversity of Microorganisms Isolated from the Soil Sample surround Chroogomphus rutilus in the Beijing Region
title_full Diversity of Microorganisms Isolated from the Soil Sample surround Chroogomphus rutilus in the Beijing Region
title_fullStr Diversity of Microorganisms Isolated from the Soil Sample surround Chroogomphus rutilus in the Beijing Region
title_full_unstemmed Diversity of Microorganisms Isolated from the Soil Sample surround Chroogomphus rutilus in the Beijing Region
title_short Diversity of Microorganisms Isolated from the Soil Sample surround Chroogomphus rutilus in the Beijing Region
title_sort diversity of microorganisms isolated from the soil sample surround chroogomphus rutilus in the beijing region
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3053533/
https://www.ncbi.nlm.nih.gov/pubmed/21448282
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