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Purification of heat-labile enterotoxin from an enterotoxin from an enterotoxigenic Escherichia coli of human origin by monoclonal immunoaffinity chromatography.
Heat-labile enterotoxin (LT) was purified from an enterotoxigenic Escherichia coli 015H11 of human origin. The purification steps included French pressure cell disruption of the bacteria, salting-out, DEAE-Sephacel on chromatography. Application of this procedure resulted in a 95.1-fold purification...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Korean Academy of Medical Sciences
1987
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3053640/ https://www.ncbi.nlm.nih.gov/pubmed/3077605 |
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author | Cho, M. J. Chang, W. H. Choi, M. S. Kim, I. S. Kang, J. S. Park, K. H. Kim, H. K. Cha, C. Y. Chung, H. K. Rhee, K. H. |
author_facet | Cho, M. J. Chang, W. H. Choi, M. S. Kim, I. S. Kang, J. S. Park, K. H. Kim, H. K. Cha, C. Y. Chung, H. K. Rhee, K. H. |
author_sort | Cho, M. J. |
collection | PubMed |
description | Heat-labile enterotoxin (LT) was purified from an enterotoxigenic Escherichia coli 015H11 of human origin. The purification steps included French pressure cell disruption of the bacteria, salting-out, DEAE-Sephacel on chromatography. Application of this procedure resulted in a 95.1-fold purification of LT with a yield of 19.9% as determined by rabbit ileal loop assay. The final LT preparation showed only one protein-staining band on polyacrylamide gel electrophoresis, indicating that the purified LT was homogeneous. |
format | Text |
id | pubmed-3053640 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1987 |
publisher | Korean Academy of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-30536402011-03-16 Purification of heat-labile enterotoxin from an enterotoxin from an enterotoxigenic Escherichia coli of human origin by monoclonal immunoaffinity chromatography. Cho, M. J. Chang, W. H. Choi, M. S. Kim, I. S. Kang, J. S. Park, K. H. Kim, H. K. Cha, C. Y. Chung, H. K. Rhee, K. H. J Korean Med Sci Research Article Heat-labile enterotoxin (LT) was purified from an enterotoxigenic Escherichia coli 015H11 of human origin. The purification steps included French pressure cell disruption of the bacteria, salting-out, DEAE-Sephacel on chromatography. Application of this procedure resulted in a 95.1-fold purification of LT with a yield of 19.9% as determined by rabbit ileal loop assay. The final LT preparation showed only one protein-staining band on polyacrylamide gel electrophoresis, indicating that the purified LT was homogeneous. Korean Academy of Medical Sciences 1987-03 /pmc/articles/PMC3053640/ /pubmed/3077605 Text en |
spellingShingle | Research Article Cho, M. J. Chang, W. H. Choi, M. S. Kim, I. S. Kang, J. S. Park, K. H. Kim, H. K. Cha, C. Y. Chung, H. K. Rhee, K. H. Purification of heat-labile enterotoxin from an enterotoxin from an enterotoxigenic Escherichia coli of human origin by monoclonal immunoaffinity chromatography. |
title | Purification of heat-labile enterotoxin from an enterotoxin from an enterotoxigenic Escherichia coli of human origin by monoclonal immunoaffinity chromatography. |
title_full | Purification of heat-labile enterotoxin from an enterotoxin from an enterotoxigenic Escherichia coli of human origin by monoclonal immunoaffinity chromatography. |
title_fullStr | Purification of heat-labile enterotoxin from an enterotoxin from an enterotoxigenic Escherichia coli of human origin by monoclonal immunoaffinity chromatography. |
title_full_unstemmed | Purification of heat-labile enterotoxin from an enterotoxin from an enterotoxigenic Escherichia coli of human origin by monoclonal immunoaffinity chromatography. |
title_short | Purification of heat-labile enterotoxin from an enterotoxin from an enterotoxigenic Escherichia coli of human origin by monoclonal immunoaffinity chromatography. |
title_sort | purification of heat-labile enterotoxin from an enterotoxin from an enterotoxigenic escherichia coli of human origin by monoclonal immunoaffinity chromatography. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3053640/ https://www.ncbi.nlm.nih.gov/pubmed/3077605 |
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