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Genetic study of hydatidiform moles by restriction fragment length polymorphisms (RFLPs) analysis.

Twenty three hydatidiform moles (HMs) were studied using the techniques of "RFLPs" employing a minisatellite deoxyribonucleic acid probe. Among the 23 HMs, 17 were homozygous types resulting from a duplicated haploid sperm, and two were heterozygous types resulting from fertilization two i...

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Detalles Bibliográficos
Autores principales: Cho, S., Kim, S. J.
Formato: Texto
Lenguaje:English
Publicado: Korean Academy of Medical Sciences 1993
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3053878/
https://www.ncbi.nlm.nih.gov/pubmed/7910025
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author Cho, S.
Kim, S. J.
author_facet Cho, S.
Kim, S. J.
author_sort Cho, S.
collection PubMed
description Twenty three hydatidiform moles (HMs) were studied using the techniques of "RFLPs" employing a minisatellite deoxyribonucleic acid probe. Among the 23 HMs, 17 were homozygous types resulting from a duplicated haploid sperm, and two were heterozygous types resulting from fertilization two independent sperms (dispermy). It was revealed that the four histopathologically diagnosed complete HMs (CHMs) were partial HMs (PHMs) with one maternal and 2 paternal chromosome contribution (diandry) or two maternal and 1 paternal alleles (digyny). The locus specific minisatellite probes were useful in classifying CHM into heterozygous and homozygous types as well as in diagnosing PHM. One heterozygous (50%) and 5 homozygous (29.4%) CHMs, and one PHM (25%) progressed to persistent gestational trophoblastic disease (p > 0.5).
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spelling pubmed-30538782011-03-16 Genetic study of hydatidiform moles by restriction fragment length polymorphisms (RFLPs) analysis. Cho, S. Kim, S. J. J Korean Med Sci Research Article Twenty three hydatidiform moles (HMs) were studied using the techniques of "RFLPs" employing a minisatellite deoxyribonucleic acid probe. Among the 23 HMs, 17 were homozygous types resulting from a duplicated haploid sperm, and two were heterozygous types resulting from fertilization two independent sperms (dispermy). It was revealed that the four histopathologically diagnosed complete HMs (CHMs) were partial HMs (PHMs) with one maternal and 2 paternal chromosome contribution (diandry) or two maternal and 1 paternal alleles (digyny). The locus specific minisatellite probes were useful in classifying CHM into heterozygous and homozygous types as well as in diagnosing PHM. One heterozygous (50%) and 5 homozygous (29.4%) CHMs, and one PHM (25%) progressed to persistent gestational trophoblastic disease (p > 0.5). Korean Academy of Medical Sciences 1993-12 /pmc/articles/PMC3053878/ /pubmed/7910025 Text en
spellingShingle Research Article
Cho, S.
Kim, S. J.
Genetic study of hydatidiform moles by restriction fragment length polymorphisms (RFLPs) analysis.
title Genetic study of hydatidiform moles by restriction fragment length polymorphisms (RFLPs) analysis.
title_full Genetic study of hydatidiform moles by restriction fragment length polymorphisms (RFLPs) analysis.
title_fullStr Genetic study of hydatidiform moles by restriction fragment length polymorphisms (RFLPs) analysis.
title_full_unstemmed Genetic study of hydatidiform moles by restriction fragment length polymorphisms (RFLPs) analysis.
title_short Genetic study of hydatidiform moles by restriction fragment length polymorphisms (RFLPs) analysis.
title_sort genetic study of hydatidiform moles by restriction fragment length polymorphisms (rflps) analysis.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3053878/
https://www.ncbi.nlm.nih.gov/pubmed/7910025
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