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Lithium-induced inhibition of Na-K ATPase and Ca ATPase activities in rat brain synaptosome.

To explore the action mechanism of lithium in the brain, the author investigated the effects of lithium on Na-K ATPase and Ca ATPase in rat brain synaptosomes prepared from forebrains by the method of Booth and Clark. The activities of Na-K ATPase and Ca ATPase were assayed by the level of inorganic...

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Detalles Bibliográficos
Autor principal: Cho, Y. W.
Formato: Texto
Lenguaje:English
Publicado: Korean Academy of Medical Sciences 1995
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054122/
https://www.ncbi.nlm.nih.gov/pubmed/7598829
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author Cho, Y. W.
author_facet Cho, Y. W.
author_sort Cho, Y. W.
collection PubMed
description To explore the action mechanism of lithium in the brain, the author investigated the effects of lithium on Na-K ATPase and Ca ATPase in rat brain synaptosomes prepared from forebrains by the method of Booth and Clark. The activities of Na-K ATPase and Ca ATPase were assayed by the level of inorganic phosphate liberated from the hydrolysis of ATP. Lithium at the optimum therapeutic concentration of 1 mM decreased the activity of Na-K ATPase from the control value of 19.08 +/- 0.29 to 18.27 +/- 0.10 micromoles Pi/mg protein/h and also reduced the activity of Ca ATPase from 6.38 +/- 0.12 to 5.64 +/- 0.12 micromoles Pi/mg protein/h. The decreased activity of Na-K ATPase will decrease the rate of Ca2+ efflux, probably via an Na-Ca exchange mechanism and will increase the rate of Ca2+ entry by the depolarization of nerve terminals. The reduced activity of Ca ATPase will result in the decreased efflux of Ca2+. As a Conclusion, it can be speculated that lithium elevates the intrasynaptosomal Ca2+ concentration via inhibition of the activities of Na-K ATPase and Ca ATPase, and this increased [Ca2+]i will cause the release of neurotransmitters and neurological effects of lithium.
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spelling pubmed-30541222011-03-15 Lithium-induced inhibition of Na-K ATPase and Ca ATPase activities in rat brain synaptosome. Cho, Y. W. J Korean Med Sci Research Article To explore the action mechanism of lithium in the brain, the author investigated the effects of lithium on Na-K ATPase and Ca ATPase in rat brain synaptosomes prepared from forebrains by the method of Booth and Clark. The activities of Na-K ATPase and Ca ATPase were assayed by the level of inorganic phosphate liberated from the hydrolysis of ATP. Lithium at the optimum therapeutic concentration of 1 mM decreased the activity of Na-K ATPase from the control value of 19.08 +/- 0.29 to 18.27 +/- 0.10 micromoles Pi/mg protein/h and also reduced the activity of Ca ATPase from 6.38 +/- 0.12 to 5.64 +/- 0.12 micromoles Pi/mg protein/h. The decreased activity of Na-K ATPase will decrease the rate of Ca2+ efflux, probably via an Na-Ca exchange mechanism and will increase the rate of Ca2+ entry by the depolarization of nerve terminals. The reduced activity of Ca ATPase will result in the decreased efflux of Ca2+. As a Conclusion, it can be speculated that lithium elevates the intrasynaptosomal Ca2+ concentration via inhibition of the activities of Na-K ATPase and Ca ATPase, and this increased [Ca2+]i will cause the release of neurotransmitters and neurological effects of lithium. Korean Academy of Medical Sciences 1995-02 /pmc/articles/PMC3054122/ /pubmed/7598829 Text en
spellingShingle Research Article
Cho, Y. W.
Lithium-induced inhibition of Na-K ATPase and Ca ATPase activities in rat brain synaptosome.
title Lithium-induced inhibition of Na-K ATPase and Ca ATPase activities in rat brain synaptosome.
title_full Lithium-induced inhibition of Na-K ATPase and Ca ATPase activities in rat brain synaptosome.
title_fullStr Lithium-induced inhibition of Na-K ATPase and Ca ATPase activities in rat brain synaptosome.
title_full_unstemmed Lithium-induced inhibition of Na-K ATPase and Ca ATPase activities in rat brain synaptosome.
title_short Lithium-induced inhibition of Na-K ATPase and Ca ATPase activities in rat brain synaptosome.
title_sort lithium-induced inhibition of na-k atpase and ca atpase activities in rat brain synaptosome.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054122/
https://www.ncbi.nlm.nih.gov/pubmed/7598829
work_keys_str_mv AT choyw lithiuminducedinhibitionofnakatpaseandcaatpaseactivitiesinratbrainsynaptosome