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Reconstitution of class I MHC molecules expressed in E. coli and complexed with single antigenic peptides.
The HLA-Cw3 heavy chain has been expressed at high level as insoluble protein aggregates in E. coli. The protein aggregates dissolved in strong denaturant solution were efficiently reconstituted by removal of denaturant in the presence of an HLA-Cw3 binding peptide (FAM) and beta 2m. The reconstitut...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Korean Academy of Medical Sciences
1997
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054208/ https://www.ncbi.nlm.nih.gov/pubmed/9288625 |