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Suppression of NF-kappaB activation in normal T cells by supernatant fluid from human renal cell carcinomas.
T lymphocytes from patients with renal cell carcinoma (RCC) show reduced immune function and impaired activation of the transcription factor, NF-kappaB. We determined the mechanism of NF-kappaB suppression in T cells of RCC patient and determined whether supernatant fluid from RCC explants (RCC-S) i...
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Formato: | Texto |
Lenguaje: | English |
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Korean Academy of Medical Sciences
1999
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054373/ https://www.ncbi.nlm.nih.gov/pubmed/10402173 |
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author | Kim, H. J. Park, J. K. Kim, Y. G. |
author_facet | Kim, H. J. Park, J. K. Kim, Y. G. |
author_sort | Kim, H. J. |
collection | PubMed |
description | T lymphocytes from patients with renal cell carcinoma (RCC) show reduced immune function and impaired activation of the transcription factor, NF-kappaB. We determined the mechanism of NF-kappaB suppression in T cells of RCC patient and determined whether supernatant fluid from RCC explants (RCC-S) induced the same phenotype of NF-kappaB suppression in normal T cells that is observed in patient T cells. The pattern of kappaB-binding activity in T cells of RCC patient was altered as compared to that seen in T cells obtained from normal volunteers. In some patients, no activation of RelA/NFkappaB1-binding activity was detectable, while in others kappaB-binding activity was modestly induced but the duration was reduced. IkappaBalpha was degraded normally following stimulation in both normal controls and T cells from RCC patients. RCC-S did not alter the cytoplasmic levels of RelA and NF-kappaB1 but did suppress their nuclear localization and inhibited the activation of RelA/NF-kappaB1 binding complexes. These results show that RCC-S can induce in normal T cells the same phenotype of impaired NF-kappaB activation that is detected in T cells of RCC patient. It also appears that NF-kappaB suppression by RCC-S may contribute to the immunosuppression of host immunity. |
format | Text |
id | pubmed-3054373 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1999 |
publisher | Korean Academy of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-30543732011-03-15 Suppression of NF-kappaB activation in normal T cells by supernatant fluid from human renal cell carcinomas. Kim, H. J. Park, J. K. Kim, Y. G. J Korean Med Sci Research Article T lymphocytes from patients with renal cell carcinoma (RCC) show reduced immune function and impaired activation of the transcription factor, NF-kappaB. We determined the mechanism of NF-kappaB suppression in T cells of RCC patient and determined whether supernatant fluid from RCC explants (RCC-S) induced the same phenotype of NF-kappaB suppression in normal T cells that is observed in patient T cells. The pattern of kappaB-binding activity in T cells of RCC patient was altered as compared to that seen in T cells obtained from normal volunteers. In some patients, no activation of RelA/NFkappaB1-binding activity was detectable, while in others kappaB-binding activity was modestly induced but the duration was reduced. IkappaBalpha was degraded normally following stimulation in both normal controls and T cells from RCC patients. RCC-S did not alter the cytoplasmic levels of RelA and NF-kappaB1 but did suppress their nuclear localization and inhibited the activation of RelA/NF-kappaB1 binding complexes. These results show that RCC-S can induce in normal T cells the same phenotype of impaired NF-kappaB activation that is detected in T cells of RCC patient. It also appears that NF-kappaB suppression by RCC-S may contribute to the immunosuppression of host immunity. Korean Academy of Medical Sciences 1999-06 /pmc/articles/PMC3054373/ /pubmed/10402173 Text en |
spellingShingle | Research Article Kim, H. J. Park, J. K. Kim, Y. G. Suppression of NF-kappaB activation in normal T cells by supernatant fluid from human renal cell carcinomas. |
title | Suppression of NF-kappaB activation in normal T cells by supernatant fluid from human renal cell carcinomas. |
title_full | Suppression of NF-kappaB activation in normal T cells by supernatant fluid from human renal cell carcinomas. |
title_fullStr | Suppression of NF-kappaB activation in normal T cells by supernatant fluid from human renal cell carcinomas. |
title_full_unstemmed | Suppression of NF-kappaB activation in normal T cells by supernatant fluid from human renal cell carcinomas. |
title_short | Suppression of NF-kappaB activation in normal T cells by supernatant fluid from human renal cell carcinomas. |
title_sort | suppression of nf-kappab activation in normal t cells by supernatant fluid from human renal cell carcinomas. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054373/ https://www.ncbi.nlm.nih.gov/pubmed/10402173 |
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