Activated platelets induce secretion of interleukin-1beta, monocyte chemotactic protein-1, and macrophage inflammatory protein-1alpha and surface expression of intercellular adhesion molecule-1 on cultured endothelial cells.

Atherosclerosis is an inflammatory disease. Platelet-endothelium interaction plays an important role in the pathophysiology of atherogenesis. We investigated the role of activated platelets for secretion of interleukin (IL)-1beta, monocyte chemotactic protein (MCP)-1 and macrophage inflammatory prot...

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Autores principales: Cha, J. K., Jeong, M. H., Bae, H. R., Han, J. Y., Jeong, S. J., Jin, H. J., Lim, Y. J., Kim, S. H., Kim, J. W.
Formato: Texto
Lenguaje:English
Publicado: Korean Academy of Medical Sciences 2000
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054638/
https://www.ncbi.nlm.nih.gov/pubmed/10895967
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author Cha, J. K.
Jeong, M. H.
Bae, H. R.
Han, J. Y.
Jeong, S. J.
Jin, H. J.
Lim, Y. J.
Kim, S. H.
Kim, J. W.
author_facet Cha, J. K.
Jeong, M. H.
Bae, H. R.
Han, J. Y.
Jeong, S. J.
Jin, H. J.
Lim, Y. J.
Kim, S. H.
Kim, J. W.
author_sort Cha, J. K.
collection PubMed
description Atherosclerosis is an inflammatory disease. Platelet-endothelium interaction plays an important role in the pathophysiology of atherogenesis. We investigated the role of activated platelets for secretion of interleukin (IL)-1beta, monocyte chemotactic protein (MCP)-1 and macrophage inflammatory protein (MIP)-1alpha and expression of intercellular adhesion molecule (ICAM)-1 on endothelial cells. Human umbilical vein endothelial cells (HUVEC) were incubated with non-stimulated or ADP-activated platelets for 6 hr. Secretion of interleukin (IL)-1beta, MCP-1 and MIP-1alpha and surface expression of ICAM-1 were measured by ELISA and flow cytometry. In the presence of activated platelets, the secretion of IL-1beta, MCP-1, and MIP-1alpha and surface expression of ICAM-1 were significantly increased compared with non-activated platelets. The present study shows that activated platelets may contribute to expression of various inflammatory mediators on endothelial cells.
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spelling pubmed-30546382011-03-15 Activated platelets induce secretion of interleukin-1beta, monocyte chemotactic protein-1, and macrophage inflammatory protein-1alpha and surface expression of intercellular adhesion molecule-1 on cultured endothelial cells. Cha, J. K. Jeong, M. H. Bae, H. R. Han, J. Y. Jeong, S. J. Jin, H. J. Lim, Y. J. Kim, S. H. Kim, J. W. J Korean Med Sci Research Article Atherosclerosis is an inflammatory disease. Platelet-endothelium interaction plays an important role in the pathophysiology of atherogenesis. We investigated the role of activated platelets for secretion of interleukin (IL)-1beta, monocyte chemotactic protein (MCP)-1 and macrophage inflammatory protein (MIP)-1alpha and expression of intercellular adhesion molecule (ICAM)-1 on endothelial cells. Human umbilical vein endothelial cells (HUVEC) were incubated with non-stimulated or ADP-activated platelets for 6 hr. Secretion of interleukin (IL)-1beta, MCP-1 and MIP-1alpha and surface expression of ICAM-1 were measured by ELISA and flow cytometry. In the presence of activated platelets, the secretion of IL-1beta, MCP-1, and MIP-1alpha and surface expression of ICAM-1 were significantly increased compared with non-activated platelets. The present study shows that activated platelets may contribute to expression of various inflammatory mediators on endothelial cells. Korean Academy of Medical Sciences 2000-06 /pmc/articles/PMC3054638/ /pubmed/10895967 Text en
spellingShingle Research Article
Cha, J. K.
Jeong, M. H.
Bae, H. R.
Han, J. Y.
Jeong, S. J.
Jin, H. J.
Lim, Y. J.
Kim, S. H.
Kim, J. W.
Activated platelets induce secretion of interleukin-1beta, monocyte chemotactic protein-1, and macrophage inflammatory protein-1alpha and surface expression of intercellular adhesion molecule-1 on cultured endothelial cells.
title Activated platelets induce secretion of interleukin-1beta, monocyte chemotactic protein-1, and macrophage inflammatory protein-1alpha and surface expression of intercellular adhesion molecule-1 on cultured endothelial cells.
title_full Activated platelets induce secretion of interleukin-1beta, monocyte chemotactic protein-1, and macrophage inflammatory protein-1alpha and surface expression of intercellular adhesion molecule-1 on cultured endothelial cells.
title_fullStr Activated platelets induce secretion of interleukin-1beta, monocyte chemotactic protein-1, and macrophage inflammatory protein-1alpha and surface expression of intercellular adhesion molecule-1 on cultured endothelial cells.
title_full_unstemmed Activated platelets induce secretion of interleukin-1beta, monocyte chemotactic protein-1, and macrophage inflammatory protein-1alpha and surface expression of intercellular adhesion molecule-1 on cultured endothelial cells.
title_short Activated platelets induce secretion of interleukin-1beta, monocyte chemotactic protein-1, and macrophage inflammatory protein-1alpha and surface expression of intercellular adhesion molecule-1 on cultured endothelial cells.
title_sort activated platelets induce secretion of interleukin-1beta, monocyte chemotactic protein-1, and macrophage inflammatory protein-1alpha and surface expression of intercellular adhesion molecule-1 on cultured endothelial cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054638/
https://www.ncbi.nlm.nih.gov/pubmed/10895967
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