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Apoptosis of skeletal muscle on steroid-induced myopathy in rats.

Recently apoptotic cell death has been reported in differentiated skeletal muscle, where apoptosis was generally assumed not to occur. To investigate whether apoptosis may contribute to the steroid-induced myopathy, rats treated with triamcinolone acetonide (TA) for 9 days were sacrificed for detect...

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Detalles Bibliográficos
Autores principales: Lee, M. J., Lee, J. S., Lee, M. C.
Formato: Texto
Lenguaje:English
Publicado: Korean Academy of Medical Sciences 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054783/
https://www.ncbi.nlm.nih.gov/pubmed/11511793
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author Lee, M. J.
Lee, J. S.
Lee, M. C.
author_facet Lee, M. J.
Lee, J. S.
Lee, M. C.
author_sort Lee, M. J.
collection PubMed
description Recently apoptotic cell death has been reported in differentiated skeletal muscle, where apoptosis was generally assumed not to occur. To investigate whether apoptosis may contribute to the steroid-induced myopathy, rats treated with triamcinolone acetonide (TA) for 9 days were sacrificed for detecting apoptosis by in situ end labeling (ISEL) and electron microscopy in the soleus muscles. Immunohistochemical stainings of Fas antigen and p53 protein were performed to examine whether apoptosis-related proteins were present in the myopathy. Muscle fiber necrosis and apoptotic myonuclei appeared in the soleus muscles following administration of TA, while control muscles showed no evidences for apoptosis. Fas antigen was not detected in control muscles, but expressed in the soleus muscles of steroid-induced myopathy. Some of the Fas antigen-expressing muscle fibers were positive for ISEL. p53 protein was not detected in any muscle fibers. These findings indicate that TA can induce apoptosis in differentiated skeletal muscles, and Fas antigen might be partly related to apoptotic muscle death in steroid-induced myopathy.
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spelling pubmed-30547832011-03-15 Apoptosis of skeletal muscle on steroid-induced myopathy in rats. Lee, M. J. Lee, J. S. Lee, M. C. J Korean Med Sci Research Article Recently apoptotic cell death has been reported in differentiated skeletal muscle, where apoptosis was generally assumed not to occur. To investigate whether apoptosis may contribute to the steroid-induced myopathy, rats treated with triamcinolone acetonide (TA) for 9 days were sacrificed for detecting apoptosis by in situ end labeling (ISEL) and electron microscopy in the soleus muscles. Immunohistochemical stainings of Fas antigen and p53 protein were performed to examine whether apoptosis-related proteins were present in the myopathy. Muscle fiber necrosis and apoptotic myonuclei appeared in the soleus muscles following administration of TA, while control muscles showed no evidences for apoptosis. Fas antigen was not detected in control muscles, but expressed in the soleus muscles of steroid-induced myopathy. Some of the Fas antigen-expressing muscle fibers were positive for ISEL. p53 protein was not detected in any muscle fibers. These findings indicate that TA can induce apoptosis in differentiated skeletal muscles, and Fas antigen might be partly related to apoptotic muscle death in steroid-induced myopathy. Korean Academy of Medical Sciences 2001-08 /pmc/articles/PMC3054783/ /pubmed/11511793 Text en
spellingShingle Research Article
Lee, M. J.
Lee, J. S.
Lee, M. C.
Apoptosis of skeletal muscle on steroid-induced myopathy in rats.
title Apoptosis of skeletal muscle on steroid-induced myopathy in rats.
title_full Apoptosis of skeletal muscle on steroid-induced myopathy in rats.
title_fullStr Apoptosis of skeletal muscle on steroid-induced myopathy in rats.
title_full_unstemmed Apoptosis of skeletal muscle on steroid-induced myopathy in rats.
title_short Apoptosis of skeletal muscle on steroid-induced myopathy in rats.
title_sort apoptosis of skeletal muscle on steroid-induced myopathy in rats.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054783/
https://www.ncbi.nlm.nih.gov/pubmed/11511793
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