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Hypermethylation of tumor-related genes in genitourinary cancer cell lines.
Hypermethylation of CpG island is a common mechanism for the inactivation of tumor-related genes. In the present study, we analyzed 13 genitourinary cancer cell lines for aberrant DNA methylation of 5 tumor-related genes using methylation- specific polymerase chain reaction (MSP). GSTP1 was methylat...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Korean Academy of Medical Sciences
2001
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054792/ https://www.ncbi.nlm.nih.gov/pubmed/11748358 |
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author | Chung, W. B. Hong, S. H. Kim, J. A. Sohn, Y. K. Kim, B. W. Kim, J. W. |
author_facet | Chung, W. B. Hong, S. H. Kim, J. A. Sohn, Y. K. Kim, B. W. Kim, J. W. |
author_sort | Chung, W. B. |
collection | PubMed |
description | Hypermethylation of CpG island is a common mechanism for the inactivation of tumor-related genes. In the present study, we analyzed 13 genitourinary cancer cell lines for aberrant DNA methylation of 5 tumor-related genes using methylation- specific polymerase chain reaction (MSP). GSTP1 was methylated in 5 (38.5%), E-cadherin in 1 (8%), VHL in 1 (8%), and MGMT and hMLH1 in none (0%). Six out of thirteen genitourinary cancer cell lines had methylation of at least one of five genes; 5 had one gene methylated, and, 1 had two genes methylated. Methylation of these 5 genes was not detected in any of the bladder cancer cell lines. GSTP1 was methylated in all of the 3 prostate cancer cell lines. We conclude that aberrant hypermethylation may be an important mechanism for the inactivation of cancer-related genes in kidney and prostate cancer cell lines. |
format | Text |
id | pubmed-3054792 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | Korean Academy of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-30547922011-03-15 Hypermethylation of tumor-related genes in genitourinary cancer cell lines. Chung, W. B. Hong, S. H. Kim, J. A. Sohn, Y. K. Kim, B. W. Kim, J. W. J Korean Med Sci Research Article Hypermethylation of CpG island is a common mechanism for the inactivation of tumor-related genes. In the present study, we analyzed 13 genitourinary cancer cell lines for aberrant DNA methylation of 5 tumor-related genes using methylation- specific polymerase chain reaction (MSP). GSTP1 was methylated in 5 (38.5%), E-cadherin in 1 (8%), VHL in 1 (8%), and MGMT and hMLH1 in none (0%). Six out of thirteen genitourinary cancer cell lines had methylation of at least one of five genes; 5 had one gene methylated, and, 1 had two genes methylated. Methylation of these 5 genes was not detected in any of the bladder cancer cell lines. GSTP1 was methylated in all of the 3 prostate cancer cell lines. We conclude that aberrant hypermethylation may be an important mechanism for the inactivation of cancer-related genes in kidney and prostate cancer cell lines. Korean Academy of Medical Sciences 2001-12 /pmc/articles/PMC3054792/ /pubmed/11748358 Text en |
spellingShingle | Research Article Chung, W. B. Hong, S. H. Kim, J. A. Sohn, Y. K. Kim, B. W. Kim, J. W. Hypermethylation of tumor-related genes in genitourinary cancer cell lines. |
title | Hypermethylation of tumor-related genes in genitourinary cancer cell lines. |
title_full | Hypermethylation of tumor-related genes in genitourinary cancer cell lines. |
title_fullStr | Hypermethylation of tumor-related genes in genitourinary cancer cell lines. |
title_full_unstemmed | Hypermethylation of tumor-related genes in genitourinary cancer cell lines. |
title_short | Hypermethylation of tumor-related genes in genitourinary cancer cell lines. |
title_sort | hypermethylation of tumor-related genes in genitourinary cancer cell lines. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054792/ https://www.ncbi.nlm.nih.gov/pubmed/11748358 |
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