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Expression patterns of cytokeratins in cholesteatomas: evidence of increased migration and proliferation.

Aural cholesteatoma is characterized by invading squamous epithelia with altered growth properties. Cytokeratin (CK) expression is affected in epidermal proliferative diseases and represents the alterations of keratinocyte proliferation, differentiation, and migration. In the present study, the inte...

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Detalles Bibliográficos
Autores principales: Kim, Hyung-Jong, Tinling, Steven P., Chole, Richard A.
Formato: Texto
Lenguaje:English
Publicado: Korean Academy of Medical Sciences 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054868/
https://www.ncbi.nlm.nih.gov/pubmed/12068144
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author Kim, Hyung-Jong
Tinling, Steven P.
Chole, Richard A.
author_facet Kim, Hyung-Jong
Tinling, Steven P.
Chole, Richard A.
author_sort Kim, Hyung-Jong
collection PubMed
description Aural cholesteatoma is characterized by invading squamous epithelia with altered growth properties. Cytokeratin (CK) expression is affected in epidermal proliferative diseases and represents the alterations of keratinocyte proliferation, differentiation, and migration. In the present study, the intensity of CK immuno-expression was determined, using densitometry at various sites in experimental cholesteatoma in order to characterize changes of keratinocytes. With cholesteatoma formation, CK4, a marker for non-keratinizing epithelia, increased in the suprabasal layers of the annular external auditory canal (EAC) and at the pars tensa indicating an altered differentiation and migration of keratinocytes. CK5/6, a marker of keratinizing squamous epithelium, increased only at the pars tensa of the tympanic membrane, indicating basal keratinocyte hyperplasia. CK1/10 increased in the suprabasal layer at the annular EAC, and at the peripheral pars tensa, indicating increased terminal differentiation of keratinocytes. CK13/16, markers of differentiation and hyperproliferation, increased in suprabasal layer of the EAC, and at the peripheral pars tensa. However, it decreased in the basal layer of the EAC, indicating hyperproliferation and migration of keratinocytes. The findings of this study support the basal cell hyperplasia hypotheses for the pathogenesis of aural cholesteatoma, with regard to hyperproliferation, migration, and an altered differentiation of keratinocytes.
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spelling pubmed-30548682011-03-15 Expression patterns of cytokeratins in cholesteatomas: evidence of increased migration and proliferation. Kim, Hyung-Jong Tinling, Steven P. Chole, Richard A. J Korean Med Sci Research Article Aural cholesteatoma is characterized by invading squamous epithelia with altered growth properties. Cytokeratin (CK) expression is affected in epidermal proliferative diseases and represents the alterations of keratinocyte proliferation, differentiation, and migration. In the present study, the intensity of CK immuno-expression was determined, using densitometry at various sites in experimental cholesteatoma in order to characterize changes of keratinocytes. With cholesteatoma formation, CK4, a marker for non-keratinizing epithelia, increased in the suprabasal layers of the annular external auditory canal (EAC) and at the pars tensa indicating an altered differentiation and migration of keratinocytes. CK5/6, a marker of keratinizing squamous epithelium, increased only at the pars tensa of the tympanic membrane, indicating basal keratinocyte hyperplasia. CK1/10 increased in the suprabasal layer at the annular EAC, and at the peripheral pars tensa, indicating increased terminal differentiation of keratinocytes. CK13/16, markers of differentiation and hyperproliferation, increased in suprabasal layer of the EAC, and at the peripheral pars tensa. However, it decreased in the basal layer of the EAC, indicating hyperproliferation and migration of keratinocytes. The findings of this study support the basal cell hyperplasia hypotheses for the pathogenesis of aural cholesteatoma, with regard to hyperproliferation, migration, and an altered differentiation of keratinocytes. Korean Academy of Medical Sciences 2002-06 /pmc/articles/PMC3054868/ /pubmed/12068144 Text en
spellingShingle Research Article
Kim, Hyung-Jong
Tinling, Steven P.
Chole, Richard A.
Expression patterns of cytokeratins in cholesteatomas: evidence of increased migration and proliferation.
title Expression patterns of cytokeratins in cholesteatomas: evidence of increased migration and proliferation.
title_full Expression patterns of cytokeratins in cholesteatomas: evidence of increased migration and proliferation.
title_fullStr Expression patterns of cytokeratins in cholesteatomas: evidence of increased migration and proliferation.
title_full_unstemmed Expression patterns of cytokeratins in cholesteatomas: evidence of increased migration and proliferation.
title_short Expression patterns of cytokeratins in cholesteatomas: evidence of increased migration and proliferation.
title_sort expression patterns of cytokeratins in cholesteatomas: evidence of increased migration and proliferation.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054868/
https://www.ncbi.nlm.nih.gov/pubmed/12068144
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AT cholericharda expressionpatternsofcytokeratinsincholesteatomasevidenceofincreasedmigrationandproliferation