Cargando…

The role of nitric oxide in ocular surface cells.

The role of nitric oxide (NO) in the ocular surface remains unknown. We investigated the conditions leading to an increase of NO generation in tear and the main sources of NO in ocular surface tissue. We evaluated the dual action (cell survival or cell death) of NO depending on its amount. We measur...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Jae Chan, Park, Gun Sic, Kim, Jin Kook, Kim, Young Myeong
Formato: Texto
Lenguaje:English
Publicado: Korean Academy of Medical Sciences 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054890/
https://www.ncbi.nlm.nih.gov/pubmed/12068145
_version_ 1782200055536549888
author Kim, Jae Chan
Park, Gun Sic
Kim, Jin Kook
Kim, Young Myeong
author_facet Kim, Jae Chan
Park, Gun Sic
Kim, Jin Kook
Kim, Young Myeong
author_sort Kim, Jae Chan
collection PubMed
description The role of nitric oxide (NO) in the ocular surface remains unknown. We investigated the conditions leading to an increase of NO generation in tear and the main sources of NO in ocular surface tissue. We evaluated the dual action (cell survival or cell death) of NO depending on its amount. We measured the concentration of nitrite plus nitrate in the tears of ocular surface diseases and examined the main source of nitric oxide synthase (NOS). When cultured human corneal fibroblast were treated with NO producing donor with or without serum, the viabilities of cells was studied. We found that the main sources of NO in ocular surface tissue were corneal epithelium, fibroblast, endothelium, and inflammatory cells. Three forms of NOS (eNOS, bNOS, and iNOS) were expressed in experimentally induced inflammation. In the fibroblast culture system, the NO donor (SNAP, S-nitroso-N-acetyl-D, L-penicillamine) prevented the death of corneal fibroblast cells caused by serum deprivation in a dose dependent manner up to 500 micrometer SNAP, but a higher dose decreased cell viability. This study suggested that NO might act as a double-edged sword in ocular surface diseases depending on the degree of inflammation related with NO concentration.
format Text
id pubmed-3054890
institution National Center for Biotechnology Information
language English
publishDate 2002
publisher Korean Academy of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-30548902011-03-15 The role of nitric oxide in ocular surface cells. Kim, Jae Chan Park, Gun Sic Kim, Jin Kook Kim, Young Myeong J Korean Med Sci Research Article The role of nitric oxide (NO) in the ocular surface remains unknown. We investigated the conditions leading to an increase of NO generation in tear and the main sources of NO in ocular surface tissue. We evaluated the dual action (cell survival or cell death) of NO depending on its amount. We measured the concentration of nitrite plus nitrate in the tears of ocular surface diseases and examined the main source of nitric oxide synthase (NOS). When cultured human corneal fibroblast were treated with NO producing donor with or without serum, the viabilities of cells was studied. We found that the main sources of NO in ocular surface tissue were corneal epithelium, fibroblast, endothelium, and inflammatory cells. Three forms of NOS (eNOS, bNOS, and iNOS) were expressed in experimentally induced inflammation. In the fibroblast culture system, the NO donor (SNAP, S-nitroso-N-acetyl-D, L-penicillamine) prevented the death of corneal fibroblast cells caused by serum deprivation in a dose dependent manner up to 500 micrometer SNAP, but a higher dose decreased cell viability. This study suggested that NO might act as a double-edged sword in ocular surface diseases depending on the degree of inflammation related with NO concentration. Korean Academy of Medical Sciences 2002-06 /pmc/articles/PMC3054890/ /pubmed/12068145 Text en
spellingShingle Research Article
Kim, Jae Chan
Park, Gun Sic
Kim, Jin Kook
Kim, Young Myeong
The role of nitric oxide in ocular surface cells.
title The role of nitric oxide in ocular surface cells.
title_full The role of nitric oxide in ocular surface cells.
title_fullStr The role of nitric oxide in ocular surface cells.
title_full_unstemmed The role of nitric oxide in ocular surface cells.
title_short The role of nitric oxide in ocular surface cells.
title_sort role of nitric oxide in ocular surface cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054890/
https://www.ncbi.nlm.nih.gov/pubmed/12068145
work_keys_str_mv AT kimjaechan theroleofnitricoxideinocularsurfacecells
AT parkgunsic theroleofnitricoxideinocularsurfacecells
AT kimjinkook theroleofnitricoxideinocularsurfacecells
AT kimyoungmyeong theroleofnitricoxideinocularsurfacecells
AT kimjaechan roleofnitricoxideinocularsurfacecells
AT parkgunsic roleofnitricoxideinocularsurfacecells
AT kimjinkook roleofnitricoxideinocularsurfacecells
AT kimyoungmyeong roleofnitricoxideinocularsurfacecells