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Active immunization using dendritic cells mixed with tumor cells inhibits the growth of lymphomas.

Dendritic cells (DCs) are potent antigen-presenting cells for the induction and activation of cytotoxic T lymphocytes. We tested whether bone marrow-derived DCs are capable of inducing protective immunity against a murine lymphoma (A20). DCs were grown from tumor-bearing BALB/c mice by culturing bon...

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Autores principales: Park, Jin Ny, Suh, Cheolwon, Yang, Jehoon, Park, Jung Sun, Park, Keon Uk, Min, Young Joo, Kim, Hyo Jung, Kim, Young-Hoon, Kim, Sang-Hee
Formato: Texto
Lenguaje:English
Publicado: Korean Academy of Medical Sciences 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3055039/
https://www.ncbi.nlm.nih.gov/pubmed/12808324
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author Park, Jin Ny
Suh, Cheolwon
Yang, Jehoon
Park, Jung Sun
Park, Keon Uk
Min, Young Joo
Kim, Hyo Jung
Kim, Young-Hoon
Kim, Sang-Hee
author_facet Park, Jin Ny
Suh, Cheolwon
Yang, Jehoon
Park, Jung Sun
Park, Keon Uk
Min, Young Joo
Kim, Hyo Jung
Kim, Young-Hoon
Kim, Sang-Hee
author_sort Park, Jin Ny
collection PubMed
description Dendritic cells (DCs) are potent antigen-presenting cells for the induction and activation of cytotoxic T lymphocytes. We tested whether bone marrow-derived DCs are capable of inducing protective immunity against a murine lymphoma (A20). DCs were grown from tumor-bearing BALB/c mice by culturing bone marrow cells. BALB/c mice were injected (sc) with A20 cells on day 0. Intraperitoneal immunization with DCs mixed with lethally irradiated A20 cells were started when the tumor reached ca. 4-5 mm in diameter (Group A) or on day -7 (Group B). Booster immunizations were given every 3-4 days for four weeks. By 31 days in group A, there was a significant reduction in tumor growth in the mice immunized with DCs mixed with irradiated A20 cells as compared with the control groups (p=0.016). In group B, tumor growth was completely inhibited and there was no tumor growth following extended observations after completion of immunization. Thus, DCs mixed with irradiated tumor cells can induce an antitumor effect. This provides a rationale for the use of DCs mixed with irradiated tumor cells in immunotherapy for minimal residual disease of lymphomas.
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spelling pubmed-30550392011-03-15 Active immunization using dendritic cells mixed with tumor cells inhibits the growth of lymphomas. Park, Jin Ny Suh, Cheolwon Yang, Jehoon Park, Jung Sun Park, Keon Uk Min, Young Joo Kim, Hyo Jung Kim, Young-Hoon Kim, Sang-Hee J Korean Med Sci Research Article Dendritic cells (DCs) are potent antigen-presenting cells for the induction and activation of cytotoxic T lymphocytes. We tested whether bone marrow-derived DCs are capable of inducing protective immunity against a murine lymphoma (A20). DCs were grown from tumor-bearing BALB/c mice by culturing bone marrow cells. BALB/c mice were injected (sc) with A20 cells on day 0. Intraperitoneal immunization with DCs mixed with lethally irradiated A20 cells were started when the tumor reached ca. 4-5 mm in diameter (Group A) or on day -7 (Group B). Booster immunizations were given every 3-4 days for four weeks. By 31 days in group A, there was a significant reduction in tumor growth in the mice immunized with DCs mixed with irradiated A20 cells as compared with the control groups (p=0.016). In group B, tumor growth was completely inhibited and there was no tumor growth following extended observations after completion of immunization. Thus, DCs mixed with irradiated tumor cells can induce an antitumor effect. This provides a rationale for the use of DCs mixed with irradiated tumor cells in immunotherapy for minimal residual disease of lymphomas. Korean Academy of Medical Sciences 2003-06 /pmc/articles/PMC3055039/ /pubmed/12808324 Text en
spellingShingle Research Article
Park, Jin Ny
Suh, Cheolwon
Yang, Jehoon
Park, Jung Sun
Park, Keon Uk
Min, Young Joo
Kim, Hyo Jung
Kim, Young-Hoon
Kim, Sang-Hee
Active immunization using dendritic cells mixed with tumor cells inhibits the growth of lymphomas.
title Active immunization using dendritic cells mixed with tumor cells inhibits the growth of lymphomas.
title_full Active immunization using dendritic cells mixed with tumor cells inhibits the growth of lymphomas.
title_fullStr Active immunization using dendritic cells mixed with tumor cells inhibits the growth of lymphomas.
title_full_unstemmed Active immunization using dendritic cells mixed with tumor cells inhibits the growth of lymphomas.
title_short Active immunization using dendritic cells mixed with tumor cells inhibits the growth of lymphomas.
title_sort active immunization using dendritic cells mixed with tumor cells inhibits the growth of lymphomas.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3055039/
https://www.ncbi.nlm.nih.gov/pubmed/12808324
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