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Effect of exogenous surfactants on viability and DNA synthesis in A549, immortalized mouse type II and isolated rat alveolar type II cells
BACKGROUND: In mechanically ventilated preterm infants with respiratory distress syndrome (RDS), exogenous surfactant application has been demonstrated both to decrease DNA-synthesis but also and paradoxically to increase epithelial cell proliferation. However, the effect of exogenous surfactant has...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3055216/ https://www.ncbi.nlm.nih.gov/pubmed/21324208 http://dx.doi.org/10.1186/1471-2466-11-11 |
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author | Wemhöner, Andreas Jennings, Paul Haller, Thomas Rüdiger, Mario Simbruner, Georg |
author_facet | Wemhöner, Andreas Jennings, Paul Haller, Thomas Rüdiger, Mario Simbruner, Georg |
author_sort | Wemhöner, Andreas |
collection | PubMed |
description | BACKGROUND: In mechanically ventilated preterm infants with respiratory distress syndrome (RDS), exogenous surfactant application has been demonstrated both to decrease DNA-synthesis but also and paradoxically to increase epithelial cell proliferation. However, the effect of exogenous surfactant has not been studied directly on alveolar type II cells (ATII cells), a key cell type responsible for alveolar function and repair. OBJECTIVE: The aim of this study was to investigate the effects of two commercially available surfactant preparations on ATII cell viability and DNA synthesis. METHODS: Curosurf(® )and Alveofact(® )were applied to two ATII cell lines (human A549 and mouse iMATII cells) and to primary rat ATII cells for periods of up to 24 h. Cell viability was measured using the redox indicator resazurin and DNA synthesis was measured using BrdU incorporation. RESULTS: Curosurf(® )resulted in slightly decreased cell viability in all cell culture models. However, DNA synthesis was increased in A549 and rat ATII cells but decreased in iMATII cells. Alveofact(® )exhibited the opposite effects on A549 cells and had very mild effects on the other two cell models. CONCLUSION: This study showed that commercially available exogenous surfactants used to treat preterm infants with RDS can have profound effects on cell viability and DNA synthesis. |
format | Text |
id | pubmed-3055216 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30552162011-03-12 Effect of exogenous surfactants on viability and DNA synthesis in A549, immortalized mouse type II and isolated rat alveolar type II cells Wemhöner, Andreas Jennings, Paul Haller, Thomas Rüdiger, Mario Simbruner, Georg BMC Pulm Med Research Article BACKGROUND: In mechanically ventilated preterm infants with respiratory distress syndrome (RDS), exogenous surfactant application has been demonstrated both to decrease DNA-synthesis but also and paradoxically to increase epithelial cell proliferation. However, the effect of exogenous surfactant has not been studied directly on alveolar type II cells (ATII cells), a key cell type responsible for alveolar function and repair. OBJECTIVE: The aim of this study was to investigate the effects of two commercially available surfactant preparations on ATII cell viability and DNA synthesis. METHODS: Curosurf(® )and Alveofact(® )were applied to two ATII cell lines (human A549 and mouse iMATII cells) and to primary rat ATII cells for periods of up to 24 h. Cell viability was measured using the redox indicator resazurin and DNA synthesis was measured using BrdU incorporation. RESULTS: Curosurf(® )resulted in slightly decreased cell viability in all cell culture models. However, DNA synthesis was increased in A549 and rat ATII cells but decreased in iMATII cells. Alveofact(® )exhibited the opposite effects on A549 cells and had very mild effects on the other two cell models. CONCLUSION: This study showed that commercially available exogenous surfactants used to treat preterm infants with RDS can have profound effects on cell viability and DNA synthesis. BioMed Central 2011-02-17 /pmc/articles/PMC3055216/ /pubmed/21324208 http://dx.doi.org/10.1186/1471-2466-11-11 Text en Copyright ©2011 Wemhöner et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wemhöner, Andreas Jennings, Paul Haller, Thomas Rüdiger, Mario Simbruner, Georg Effect of exogenous surfactants on viability and DNA synthesis in A549, immortalized mouse type II and isolated rat alveolar type II cells |
title | Effect of exogenous surfactants on viability and DNA synthesis in A549, immortalized mouse type II and isolated rat alveolar type II cells |
title_full | Effect of exogenous surfactants on viability and DNA synthesis in A549, immortalized mouse type II and isolated rat alveolar type II cells |
title_fullStr | Effect of exogenous surfactants on viability and DNA synthesis in A549, immortalized mouse type II and isolated rat alveolar type II cells |
title_full_unstemmed | Effect of exogenous surfactants on viability and DNA synthesis in A549, immortalized mouse type II and isolated rat alveolar type II cells |
title_short | Effect of exogenous surfactants on viability and DNA synthesis in A549, immortalized mouse type II and isolated rat alveolar type II cells |
title_sort | effect of exogenous surfactants on viability and dna synthesis in a549, immortalized mouse type ii and isolated rat alveolar type ii cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3055216/ https://www.ncbi.nlm.nih.gov/pubmed/21324208 http://dx.doi.org/10.1186/1471-2466-11-11 |
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