Comparison of Enzymatic and Non-Enzymatic Means of Dissociating Adherent Monolayers of Mesenchymal Stem Cells

The dissociation of adherent mesenchymal stem cell (MSC) monolayers with trypsin and enzyme-free dissociation buffer was compared. A significantly lower proportion of viable cells were obtained with enzyme-free dissociation buffers compared to trypsin. Subsequently, the dissociated cells were re-see...

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Detalles Bibliográficos
Autores principales: Heng, Boon C, Cowan, Catherine M, Basu, Shubhayu
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3055293/
https://www.ncbi.nlm.nih.gov/pubmed/19495917
http://dx.doi.org/10.1007/s12575-009-9001-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3055293/
https://www.ncbi.nlm.nih.gov/pubmed/19495917
http://dx.doi.org/10.1007/s12575-009-9001-4

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