Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I

Quantization of gene expression requires that an accurate measurement of a specific transcript is made. In this paper, a quantitative reverse transcription-polymerase chain reaction (RT-PCR) by competition for tilapia growth hormone receptor type I is designed and validated. This experimental proced...

Descripción completa

Detalles Bibliográficos
Autores principales: Rodríguez-Mallon, Alina, Cárdenas, Yamilet, Lugo, Juana María, Oliva, Aymé, Morales, Antonio, Estrada, Mario Pablo
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3055623/
https://www.ncbi.nlm.nih.gov/pubmed/19495916
http://dx.doi.org/10.1007/s12575-009-9002-3
_version_ 1782200131541532672
author Rodríguez-Mallon, Alina
Cárdenas, Yamilet
Lugo, Juana María
Oliva, Aymé
Morales, Antonio
Estrada, Mario Pablo
author_facet Rodríguez-Mallon, Alina
Cárdenas, Yamilet
Lugo, Juana María
Oliva, Aymé
Morales, Antonio
Estrada, Mario Pablo
author_sort Rodríguez-Mallon, Alina
collection PubMed
description Quantization of gene expression requires that an accurate measurement of a specific transcript is made. In this paper, a quantitative reverse transcription-polymerase chain reaction (RT-PCR) by competition for tilapia growth hormone receptor type I is designed and validated. This experimental procedure was used to determine the abundance of growth hormone receptor type I transcript in different tilapia tissues. The results obtained with this developed competitive RT-PCR were similar to real-time PCR results reported recently. This protocol provides a reliable alternative, but less expensive than real-time PCR to quantify specific genes.
format Text
id pubmed-3055623
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-30556232011-03-12 Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I Rodríguez-Mallon, Alina Cárdenas, Yamilet Lugo, Juana María Oliva, Aymé Morales, Antonio Estrada, Mario Pablo Biol Proced Online Methodology Quantization of gene expression requires that an accurate measurement of a specific transcript is made. In this paper, a quantitative reverse transcription-polymerase chain reaction (RT-PCR) by competition for tilapia growth hormone receptor type I is designed and validated. This experimental procedure was used to determine the abundance of growth hormone receptor type I transcript in different tilapia tissues. The results obtained with this developed competitive RT-PCR were similar to real-time PCR results reported recently. This protocol provides a reliable alternative, but less expensive than real-time PCR to quantify specific genes. BioMed Central 2009-03-10 /pmc/articles/PMC3055623/ /pubmed/19495916 http://dx.doi.org/10.1007/s12575-009-9002-3 Text en Copyright ©2009 Rodríguez-Mallon et al. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Rodríguez-Mallon, Alina
Cárdenas, Yamilet
Lugo, Juana María
Oliva, Aymé
Morales, Antonio
Estrada, Mario Pablo
Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I
title Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I
title_full Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I
title_fullStr Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I
title_full_unstemmed Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I
title_short Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I
title_sort competitive rt-pcr strategy for quantitative evaluation of the expression of tilapia (oreochromis niloticus) growth hormone receptor type i
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3055623/
https://www.ncbi.nlm.nih.gov/pubmed/19495916
http://dx.doi.org/10.1007/s12575-009-9002-3
work_keys_str_mv AT rodriguezmallonalina competitivertpcrstrategyforquantitativeevaluationoftheexpressionoftilapiaoreochromisniloticusgrowthhormonereceptortypei
AT cardenasyamilet competitivertpcrstrategyforquantitativeevaluationoftheexpressionoftilapiaoreochromisniloticusgrowthhormonereceptortypei
AT lugojuanamaria competitivertpcrstrategyforquantitativeevaluationoftheexpressionoftilapiaoreochromisniloticusgrowthhormonereceptortypei
AT olivaayme competitivertpcrstrategyforquantitativeevaluationoftheexpressionoftilapiaoreochromisniloticusgrowthhormonereceptortypei
AT moralesantonio competitivertpcrstrategyforquantitativeevaluationoftheexpressionoftilapiaoreochromisniloticusgrowthhormonereceptortypei
AT estradamariopablo competitivertpcrstrategyforquantitativeevaluationoftheexpressionoftilapiaoreochromisniloticusgrowthhormonereceptortypei

Ejemplares similares