Cargando…

Ultra-Fast and Optimized Method for the Preparation of Rodent Testicular Cells for Flow Cytometric Analysis

Homogeneity of cell populations is a prerequisite for the analysis of biochemical and molecular events during male gamete differentiation. Given the complex organization of the mammalian testicular tissue, various methods have been used to obtain enriched or purified cell populations, including flow...

Descripción completa

Detalles Bibliográficos
Autores principales:	Rodríguez-Casuriaga, Rosana, Geisinger, Adriana, López-Carro, Beatriz, Porro, Valentina, Wettstein, Rodolfo, Folle, Gustavo A
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2009
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3055716/ https://www.ncbi.nlm.nih.gov/pubmed/19495915 http://dx.doi.org/10.1007/s12575-009-9003-2

Ejemplares similares

Rapid preparation of rodent testicular cell suspensions and spermatogenic stages purification by flow cytometry using a novel blue-laser-excitable vital dye
por: Rodríguez-Casuriaga, Rosana, et al.
Publicado: (2014)

Contributions of Flow Cytometry to the Molecular Study of Spermatogenesis in Mammals
por: Rodríguez-Casuriaga, Rosana, et al.
Publicado: (2021)

Transcriptomics of Meiosis in the Male Mouse
por: Geisinger, Adriana, et al.
Publicado: (2021)

Revealing stage-specific expression patterns of long noncoding RNAs along mouse spermatogenesis
por: Trovero, María F., et al.
Publicado: (2019)

A flow cytometric assay to quantify invasion of red blood cells by rodent Plasmodium parasites in vivo
por: Lelliott, Patrick M, et al.
Publicado: (2014)

Transcriptome analysis of highly purified mouse spermatogenic cell populations: gene expression signatures switch from meiotic-to postmeiotic-related processes at pachytene stage
por: da Cruz, Irene, et al.
Publicado: (2016)

Improved cytometric analysis of untouched lung leukocytes by enzymatic liquefaction of sputum samples
por: Santopolo, Giulia, et al.
Publicado: (2022)

MADOKA: an ultra-fast approach for large-scale protein structure similarity searching
por: Deng, Lei, et al.
Publicado: (2019)

Flow-cytometric assessment of cellular poly(ADP-ribosyl)ation capacity in peripheral blood lymphocytes
por: Kunzmann, Andrea, et al.
Publicado: (2006)

Standardization and validation of a cytometric bead assay to assess antibodies to multiple Plasmodium falciparum recombinant antigens
por: Ondigo, Bartholomew N, et al.
Publicado: (2012)

Optimization of flow cytometric detection and cell sorting of transgenic Plasmodium parasites using interchangeable optical filters
por: Vorobjev, Ivan A, et al.
Publicado: (2012)

Simple flow cytometric detection of haemozoin containing leukocytes and erythrocytes for research on diagnosis, immunology and drug sensitivity testing
por: Frita, Rosangela, et al.
Publicado: (2011)

A fast and cost-effective microsampling protocol incorporating reduced animal usage for time-series transcriptomics in rodent malaria parasites
por: Ramaprasad, Abhinay, et al.
Publicado: (2019)

A new spreadsheet method for the analysis of bivariate flow cytometric data
por: Tzircotis, George, et al.
Publicado: (2004)

flowDiv: a new pipeline for analyzing flow cytometric diversity
por: Wanderley, Bruno M. S., et al.
Publicado: (2019)

Cytometric measurement of in vitro inhibition of Plasmodium falciparum field isolates by drugs: a new approach for re-invasion inhibition study
por: Varela, Marie Louise, et al.
Publicado: (2014)

A novel flow cytometric assay for measurement of In Vivo pulmonary neutrophil phagocytosis
por: Vander Top, Elizabeth A, et al.
Publicado: (2006)

SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse
por: Capoano, Carlos A., et al.
Publicado: (2021)

Testicular Toxicity of Chloroxylenol in Rats: Biochemical, Pathological and Flow Cytometric Study
por: El-Naggar, Doaa Abdallah, et al.
Publicado: (2022)

Flow cytometric monitoring of influenza A virus infection in MDCK cells during vaccine production
por: Schulze-Horsel, Josef, et al.
Publicado: (2008)

Sequential events of apoptosis involving docetaxel, a microtubule-interfering agent: A cytometric study
por: Fabbri, Francesco, et al.
Publicado: (2006)

Flow cytometric readout based on Mitotracker Red CMXRos staining of live asexual blood stage malarial parasites reliably assesses antibody dependent cellular inhibition
por: Jogdand, Prajakta S, et al.
Publicado: (2012)

A novel ultra high-throughput 16S rRNA gene amplicon sequencing library preparation method for the Illumina HiSeq platform
por: de Muinck, Eric J., et al.
Publicado: (2017)

Development of flow cytometric opsonophagocytosis and antibody-mediated complement deposition assays for non-typeable Haemophilus influenzae
por: Thomas, Stephen R., et al.
Publicado: (2018)

Mosquito transmission of the rodent malaria parasite Plasmodium chabaudi
por: Spence, Philip J, et al.
Publicado: (2012)

The clinical significance of flow cytometric c-myc oncoprotein quantitation in testicular cancer.
por: Watson, J. V., et al.
Publicado: (1986)

Improved negative selection protocol for Plasmodium berghei in the rodent malarial model
por: Orr, Rachael Y, et al.
Publicado: (2012)

Electrically Induced Limbic Seizures: Preliminary Findings in a Rodent Model
por: Kowski, Alexander B, et al.
Publicado: (2015)

Development of a new ultra sensitive real-time PCR assay (ultra sensitive RTQ-PCR) for the quantification of HBV-DNA
por: Paraskevis, Dimitrios, et al.
Publicado: (2010)

Leukocytospermia and sperm preparation - a flow cytometric study
por: Ricci, Giuseppe, et al.
Publicado: (2009)

A method for exposing rodents to resuspended particles using whole-body plethysmography
por: Wichers, Lindsay B, et al.
Publicado: (2006)

Application of the automated haematology analyzer XN-30 in an experimental rodent model of malaria
por: Tougan, Takahiro, et al.
Publicado: (2018)

The intracellular detection of MIP-1beta enhances the capacity to detect IFN-gamma mediated HIV-1-specific CD8 T-cell responses in a flow cytometric setting providing a sensitive alternative to the ELISPOT
por: Kutscher, Sarah, et al.
Publicado: (2008)

Effect of liver growth factor on both testicular regeneration and recovery of spermatogenesis in busulfan-treated mice
por: Pérez-Crespo, Miriam, et al.
Publicado: (2011)

Ultra-light photosensor collars to monitor Arctic lemming activity
por: Bolduc, David, et al.
Publicado: (2022)

DNA Flow cytometric analysis of the human testicular tissues to investigate the status of spermatogenesis in azoospermic patients
por: Baksi, Arka, et al.
Publicado: (2018)

An Experimental Model for Resistance Exercise in Rodents
por: Nicastro, Humberto, et al.
Publicado: (2012)

Ultra-sensitive isotope probing to quantify activity and substrate assimilation in microbiomes
por: Kleiner, Manuel, et al.
Publicado: (2023)

Protocol: a fast, comprehensive and reproducible one-step extraction method for the rapid preparation of polar and semi-polar metabolites, lipids, proteins, starch and cell wall polymers from a single sample
por: Salem, Mohamed A., et al.
Publicado: (2016)

Transformation of the rodent malaria parasite Plasmodium chabaudi and generation of a stable fluorescent line PcGFP(CON)
por: Reece, Sarah E, et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_1hs79obhmc8t92i5bhaaumfqc6