Cargando…
Downregulation of TGF-beta receptor types II and III in oral squamous cell carcinoma and oral carcinoma-associated fibroblasts
BACKGROUND: The purpose of this study was to assess the expression levels for TβRI, TβRII, and TβRIII in epithelial layers of oral premalignant lesions (oral leukoplakia, OLK) and oral squamous cell carcinoma (OSCC), as well as in oral carcinoma-associated fibroblasts (CAFs), with the final goal of...
Autores principales: | , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3055848/ https://www.ncbi.nlm.nih.gov/pubmed/21352603 http://dx.doi.org/10.1186/1471-2407-11-88 |
_version_ | 1782200149798289408 |
---|---|
author | Meng, Wenxia Xia, Qingjie Wu, Lanyan Chen, Sixiu He, Xin Zhang, Lin Gao, Qinghong Zhou, Hongmei |
author_facet | Meng, Wenxia Xia, Qingjie Wu, Lanyan Chen, Sixiu He, Xin Zhang, Lin Gao, Qinghong Zhou, Hongmei |
author_sort | Meng, Wenxia |
collection | PubMed |
description | BACKGROUND: The purpose of this study was to assess the expression levels for TβRI, TβRII, and TβRIII in epithelial layers of oral premalignant lesions (oral leukoplakia, OLK) and oral squamous cell carcinoma (OSCC), as well as in oral carcinoma-associated fibroblasts (CAFs), with the final goal of exploring the roles of various types of TβRs in carcinogenesis of oral mucosa. METHODS: Normal oral tissues, OLK, and OSCC were obtained from 138 previously untreated patients. Seven primary human oral CAF lines and six primary normal fibroblast (NF) lines were established successfully via cell culture. The three receptors were detected using immunohistochemical (IHC), quantitative RT-PCR, and Western blot approaches. RESULTS: IHC signals for TβRII and TβRIII in the epithelial layer decreased in tissue samples with increasing disease aggressiveness (P < 0.05); no expression differences were observed for TβRI, in OLK and OSCC (P > 0.05); and TβRII and TβRIII were significantly downregulated in CAFs compared with NFs, at the mRNA and protein levels (P < 0.05). Exogenous expression of TGF-β1 led to a remarkable decrease in the expression of TβRII and TβRIII in CAFs (P < 0.05). CONCLUSION: This study provides the first evidence that the loss of TβRII and TβRIII expression in oral epithelium and stroma is a common event in OSCC. The restoration of the expression of TβRII and TβRIII in oral cancerous tissues may represent a novel strategy for the treatment of oral carcinoma. |
format | Text |
id | pubmed-3055848 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30558482011-03-12 Downregulation of TGF-beta receptor types II and III in oral squamous cell carcinoma and oral carcinoma-associated fibroblasts Meng, Wenxia Xia, Qingjie Wu, Lanyan Chen, Sixiu He, Xin Zhang, Lin Gao, Qinghong Zhou, Hongmei BMC Cancer Research Article BACKGROUND: The purpose of this study was to assess the expression levels for TβRI, TβRII, and TβRIII in epithelial layers of oral premalignant lesions (oral leukoplakia, OLK) and oral squamous cell carcinoma (OSCC), as well as in oral carcinoma-associated fibroblasts (CAFs), with the final goal of exploring the roles of various types of TβRs in carcinogenesis of oral mucosa. METHODS: Normal oral tissues, OLK, and OSCC were obtained from 138 previously untreated patients. Seven primary human oral CAF lines and six primary normal fibroblast (NF) lines were established successfully via cell culture. The three receptors were detected using immunohistochemical (IHC), quantitative RT-PCR, and Western blot approaches. RESULTS: IHC signals for TβRII and TβRIII in the epithelial layer decreased in tissue samples with increasing disease aggressiveness (P < 0.05); no expression differences were observed for TβRI, in OLK and OSCC (P > 0.05); and TβRII and TβRIII were significantly downregulated in CAFs compared with NFs, at the mRNA and protein levels (P < 0.05). Exogenous expression of TGF-β1 led to a remarkable decrease in the expression of TβRII and TβRIII in CAFs (P < 0.05). CONCLUSION: This study provides the first evidence that the loss of TβRII and TβRIII expression in oral epithelium and stroma is a common event in OSCC. The restoration of the expression of TβRII and TβRIII in oral cancerous tissues may represent a novel strategy for the treatment of oral carcinoma. BioMed Central 2011-02-28 /pmc/articles/PMC3055848/ /pubmed/21352603 http://dx.doi.org/10.1186/1471-2407-11-88 Text en Copyright ©2011 Meng et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Meng, Wenxia Xia, Qingjie Wu, Lanyan Chen, Sixiu He, Xin Zhang, Lin Gao, Qinghong Zhou, Hongmei Downregulation of TGF-beta receptor types II and III in oral squamous cell carcinoma and oral carcinoma-associated fibroblasts |
title | Downregulation of TGF-beta receptor types II and III in oral squamous cell carcinoma and oral carcinoma-associated fibroblasts |
title_full | Downregulation of TGF-beta receptor types II and III in oral squamous cell carcinoma and oral carcinoma-associated fibroblasts |
title_fullStr | Downregulation of TGF-beta receptor types II and III in oral squamous cell carcinoma and oral carcinoma-associated fibroblasts |
title_full_unstemmed | Downregulation of TGF-beta receptor types II and III in oral squamous cell carcinoma and oral carcinoma-associated fibroblasts |
title_short | Downregulation of TGF-beta receptor types II and III in oral squamous cell carcinoma and oral carcinoma-associated fibroblasts |
title_sort | downregulation of tgf-beta receptor types ii and iii in oral squamous cell carcinoma and oral carcinoma-associated fibroblasts |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3055848/ https://www.ncbi.nlm.nih.gov/pubmed/21352603 http://dx.doi.org/10.1186/1471-2407-11-88 |
work_keys_str_mv | AT mengwenxia downregulationoftgfbetareceptortypesiiandiiiinoralsquamouscellcarcinomaandoralcarcinomaassociatedfibroblasts AT xiaqingjie downregulationoftgfbetareceptortypesiiandiiiinoralsquamouscellcarcinomaandoralcarcinomaassociatedfibroblasts AT wulanyan downregulationoftgfbetareceptortypesiiandiiiinoralsquamouscellcarcinomaandoralcarcinomaassociatedfibroblasts AT chensixiu downregulationoftgfbetareceptortypesiiandiiiinoralsquamouscellcarcinomaandoralcarcinomaassociatedfibroblasts AT hexin downregulationoftgfbetareceptortypesiiandiiiinoralsquamouscellcarcinomaandoralcarcinomaassociatedfibroblasts AT zhanglin downregulationoftgfbetareceptortypesiiandiiiinoralsquamouscellcarcinomaandoralcarcinomaassociatedfibroblasts AT gaoqinghong downregulationoftgfbetareceptortypesiiandiiiinoralsquamouscellcarcinomaandoralcarcinomaassociatedfibroblasts AT zhouhongmei downregulationoftgfbetareceptortypesiiandiiiinoralsquamouscellcarcinomaandoralcarcinomaassociatedfibroblasts |