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A Modified Protocol for Bisulfite Genomic Sequencing of Difficult Samples

The bisulfite genomic sequencing protocol is a widely used method for analyzing DNA methylation. It relies on the deamination of unmethylated cytosine residues to uracil; however, its high rates of DNA degradation and incomplete cytosine to uracil conversion often lead to failed experiments, uninfor...

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Detalles Bibliográficos
Autores principales:	Pappas, Jane J, Toulouse, André, Bradley, WEC
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2009
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3055914/ https://www.ncbi.nlm.nih.gov/pubmed/19551458 http://dx.doi.org/10.1007/s12575-009-9010-3

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