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A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay

In order to determine the activity of O-linked GlcNAc transferase (OGT), a modified coupled enzyme method was proposed. This method was based on the measurement of uridine 5'-(trihydrogen diphosphate) (UDP), a product generated in transglycosylation reaction. In the assay, UDP was coupled to th...

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Detalles Bibliográficos
Autores principales:	Zhang, Lianwen, Ren, Feifei, Li, Jing, Ma, Xiaofeng, Wang, Peng
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2009
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3056017/ https://www.ncbi.nlm.nih.gov/pubmed/19957065 http://dx.doi.org/10.1007/s12575-009-9016-x

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