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Genetic Characteristics, Coreceptor Usage Potential and Evolution of Nigerian HIV-1 Subtype G and CRF02_AG Isolates

HIV-1 CRF02_AG and subtype G (HIV-1G) account for most HIV infections in Nigeria, but their evolutionary trends have not been well documented. To better elucidate the dynamics of the epidemic in Nigeria we characterised the gag and env genes of North-Central Nigerian HIV-1 isolates from pregnant wom...

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Autores principales: Ajoge, Hannah O., Gordon, Michelle L., de Oliveira, Tulio, Green, Taryn N., Ibrahim, Sani, Shittu, Oladapo S., Olonitola, Stephen O., Ahmad, Aliyu A., Ndung'u, Thumbi
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3056731/
https://www.ncbi.nlm.nih.gov/pubmed/21423811
http://dx.doi.org/10.1371/journal.pone.0017865
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author Ajoge, Hannah O.
Gordon, Michelle L.
de Oliveira, Tulio
Green, Taryn N.
Ibrahim, Sani
Shittu, Oladapo S.
Olonitola, Stephen O.
Ahmad, Aliyu A.
Ndung'u, Thumbi
author_facet Ajoge, Hannah O.
Gordon, Michelle L.
de Oliveira, Tulio
Green, Taryn N.
Ibrahim, Sani
Shittu, Oladapo S.
Olonitola, Stephen O.
Ahmad, Aliyu A.
Ndung'u, Thumbi
author_sort Ajoge, Hannah O.
collection PubMed
description HIV-1 CRF02_AG and subtype G (HIV-1G) account for most HIV infections in Nigeria, but their evolutionary trends have not been well documented. To better elucidate the dynamics of the epidemic in Nigeria we characterised the gag and env genes of North-Central Nigerian HIV-1 isolates from pregnant women. Of 28 samples sequenced in both genes, the predominant clades were CRF02_AG (39%) and HIV-1G (32%). Higher predicted proportion of CXCR4-tropic (X4) HIV-1G isolates was noted compared to CRF02_AG (p = 0.007, Fisher's exact test). Phylogenetic and Bayesian analysis conducted on our sequences and all the dated available Nigerian sequences on the Los Alamos data base showed that CRF02_AG and HIV-1G entered into Nigeria through multiple entries, with presence of HIV-1G dating back to early 1980s. This study underlines the genetic complexity of the HIV-1 epidemic in Nigeria, possible subtype-specific differences in co-receptor usage, and the evolutionary trends of the predominant HIV-1 strains in Nigeria, which may have implications for the design of biomedical interventions and better understanding of the epidemic.
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spelling pubmed-30567312011-03-18 Genetic Characteristics, Coreceptor Usage Potential and Evolution of Nigerian HIV-1 Subtype G and CRF02_AG Isolates Ajoge, Hannah O. Gordon, Michelle L. de Oliveira, Tulio Green, Taryn N. Ibrahim, Sani Shittu, Oladapo S. Olonitola, Stephen O. Ahmad, Aliyu A. Ndung'u, Thumbi PLoS One Research Article HIV-1 CRF02_AG and subtype G (HIV-1G) account for most HIV infections in Nigeria, but their evolutionary trends have not been well documented. To better elucidate the dynamics of the epidemic in Nigeria we characterised the gag and env genes of North-Central Nigerian HIV-1 isolates from pregnant women. Of 28 samples sequenced in both genes, the predominant clades were CRF02_AG (39%) and HIV-1G (32%). Higher predicted proportion of CXCR4-tropic (X4) HIV-1G isolates was noted compared to CRF02_AG (p = 0.007, Fisher's exact test). Phylogenetic and Bayesian analysis conducted on our sequences and all the dated available Nigerian sequences on the Los Alamos data base showed that CRF02_AG and HIV-1G entered into Nigeria through multiple entries, with presence of HIV-1G dating back to early 1980s. This study underlines the genetic complexity of the HIV-1 epidemic in Nigeria, possible subtype-specific differences in co-receptor usage, and the evolutionary trends of the predominant HIV-1 strains in Nigeria, which may have implications for the design of biomedical interventions and better understanding of the epidemic. Public Library of Science 2011-03-14 /pmc/articles/PMC3056731/ /pubmed/21423811 http://dx.doi.org/10.1371/journal.pone.0017865 Text en Ajoge et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ajoge, Hannah O.
Gordon, Michelle L.
de Oliveira, Tulio
Green, Taryn N.
Ibrahim, Sani
Shittu, Oladapo S.
Olonitola, Stephen O.
Ahmad, Aliyu A.
Ndung'u, Thumbi
Genetic Characteristics, Coreceptor Usage Potential and Evolution of Nigerian HIV-1 Subtype G and CRF02_AG Isolates
title Genetic Characteristics, Coreceptor Usage Potential and Evolution of Nigerian HIV-1 Subtype G and CRF02_AG Isolates
title_full Genetic Characteristics, Coreceptor Usage Potential and Evolution of Nigerian HIV-1 Subtype G and CRF02_AG Isolates
title_fullStr Genetic Characteristics, Coreceptor Usage Potential and Evolution of Nigerian HIV-1 Subtype G and CRF02_AG Isolates
title_full_unstemmed Genetic Characteristics, Coreceptor Usage Potential and Evolution of Nigerian HIV-1 Subtype G and CRF02_AG Isolates
title_short Genetic Characteristics, Coreceptor Usage Potential and Evolution of Nigerian HIV-1 Subtype G and CRF02_AG Isolates
title_sort genetic characteristics, coreceptor usage potential and evolution of nigerian hiv-1 subtype g and crf02_ag isolates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3056731/
https://www.ncbi.nlm.nih.gov/pubmed/21423811
http://dx.doi.org/10.1371/journal.pone.0017865
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