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Caffeoylquinic Acids Are Major Constituents with Potent Anti-Influenza Effects in Brazilian Green Propolis Water Extract

Influenza A viral infections reached pandemic levels in 1918, 1957, 1968, and, most recently, in 2009 with the emergence of the swine-origin H1N1 influenza virus. The development of novel therapeutics or prophylactics for influenza virus infection is urgently needed. We examined the evaluation of th...

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Autores principales: Urushisaki, Tomohiko, Takemura, Tomoaki, Tazawa, Shigemi, Fukuoka, Mayuko, Hosokawa-Muto, Junji, Araki, Yoko, Kuwata, Kazuo
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057164/
https://www.ncbi.nlm.nih.gov/pubmed/21423687
http://dx.doi.org/10.1155/2011/254914
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author Urushisaki, Tomohiko
Takemura, Tomoaki
Tazawa, Shigemi
Fukuoka, Mayuko
Hosokawa-Muto, Junji
Araki, Yoko
Kuwata, Kazuo
author_facet Urushisaki, Tomohiko
Takemura, Tomoaki
Tazawa, Shigemi
Fukuoka, Mayuko
Hosokawa-Muto, Junji
Araki, Yoko
Kuwata, Kazuo
author_sort Urushisaki, Tomohiko
collection PubMed
description Influenza A viral infections reached pandemic levels in 1918, 1957, 1968, and, most recently, in 2009 with the emergence of the swine-origin H1N1 influenza virus. The development of novel therapeutics or prophylactics for influenza virus infection is urgently needed. We examined the evaluation of the anti-influenza virus (A/WSN/33 (H1N1)) activity of Brazilian green propolis water extract (PWE) and its constituents by cell viability and real-time PCR assays. Our findings showed strong evidence that PWE has an anti-influenza effect and demonstrate that caffeoylquinic acids are the active anti-influenza components of PWE. Furthermore, we have found that the amount of viral RNA per cell remained unchanged even in the presence of PWE, suggesting that PWE has no direct impact on the influenza virus but may have a cytoprotective activity by affecting internal cellular process. These findings indicate that caffeoylquinic acids are the active anti-influenza components of PWE. Above findings might facilitate the prophylactic application of natural products and the realization of novel anti-influenza drugs based on caffeoylquinic acids, as well as further the understanding of cytoprotective intracellular mechanisms in influenza virus-infected cells.
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spelling pubmed-30571642011-03-21 Caffeoylquinic Acids Are Major Constituents with Potent Anti-Influenza Effects in Brazilian Green Propolis Water Extract Urushisaki, Tomohiko Takemura, Tomoaki Tazawa, Shigemi Fukuoka, Mayuko Hosokawa-Muto, Junji Araki, Yoko Kuwata, Kazuo Evid Based Complement Alternat Med Research Article Influenza A viral infections reached pandemic levels in 1918, 1957, 1968, and, most recently, in 2009 with the emergence of the swine-origin H1N1 influenza virus. The development of novel therapeutics or prophylactics for influenza virus infection is urgently needed. We examined the evaluation of the anti-influenza virus (A/WSN/33 (H1N1)) activity of Brazilian green propolis water extract (PWE) and its constituents by cell viability and real-time PCR assays. Our findings showed strong evidence that PWE has an anti-influenza effect and demonstrate that caffeoylquinic acids are the active anti-influenza components of PWE. Furthermore, we have found that the amount of viral RNA per cell remained unchanged even in the presence of PWE, suggesting that PWE has no direct impact on the influenza virus but may have a cytoprotective activity by affecting internal cellular process. These findings indicate that caffeoylquinic acids are the active anti-influenza components of PWE. Above findings might facilitate the prophylactic application of natural products and the realization of novel anti-influenza drugs based on caffeoylquinic acids, as well as further the understanding of cytoprotective intracellular mechanisms in influenza virus-infected cells. Hindawi Publishing Corporation 2011 2011-03-01 /pmc/articles/PMC3057164/ /pubmed/21423687 http://dx.doi.org/10.1155/2011/254914 Text en Copyright © 2011 Tomohiko Urushisaki et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Urushisaki, Tomohiko
Takemura, Tomoaki
Tazawa, Shigemi
Fukuoka, Mayuko
Hosokawa-Muto, Junji
Araki, Yoko
Kuwata, Kazuo
Caffeoylquinic Acids Are Major Constituents with Potent Anti-Influenza Effects in Brazilian Green Propolis Water Extract
title Caffeoylquinic Acids Are Major Constituents with Potent Anti-Influenza Effects in Brazilian Green Propolis Water Extract
title_full Caffeoylquinic Acids Are Major Constituents with Potent Anti-Influenza Effects in Brazilian Green Propolis Water Extract
title_fullStr Caffeoylquinic Acids Are Major Constituents with Potent Anti-Influenza Effects in Brazilian Green Propolis Water Extract
title_full_unstemmed Caffeoylquinic Acids Are Major Constituents with Potent Anti-Influenza Effects in Brazilian Green Propolis Water Extract
title_short Caffeoylquinic Acids Are Major Constituents with Potent Anti-Influenza Effects in Brazilian Green Propolis Water Extract
title_sort caffeoylquinic acids are major constituents with potent anti-influenza effects in brazilian green propolis water extract
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057164/
https://www.ncbi.nlm.nih.gov/pubmed/21423687
http://dx.doi.org/10.1155/2011/254914
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