Cargando…
Caffeoylquinic Acids Are Major Constituents with Potent Anti-Influenza Effects in Brazilian Green Propolis Water Extract
Influenza A viral infections reached pandemic levels in 1918, 1957, 1968, and, most recently, in 2009 with the emergence of the swine-origin H1N1 influenza virus. The development of novel therapeutics or prophylactics for influenza virus infection is urgently needed. We examined the evaluation of th...
Autores principales: | , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057164/ https://www.ncbi.nlm.nih.gov/pubmed/21423687 http://dx.doi.org/10.1155/2011/254914 |
_version_ | 1782200268096536576 |
---|---|
author | Urushisaki, Tomohiko Takemura, Tomoaki Tazawa, Shigemi Fukuoka, Mayuko Hosokawa-Muto, Junji Araki, Yoko Kuwata, Kazuo |
author_facet | Urushisaki, Tomohiko Takemura, Tomoaki Tazawa, Shigemi Fukuoka, Mayuko Hosokawa-Muto, Junji Araki, Yoko Kuwata, Kazuo |
author_sort | Urushisaki, Tomohiko |
collection | PubMed |
description | Influenza A viral infections reached pandemic levels in 1918, 1957, 1968, and, most recently, in 2009 with the emergence of the swine-origin H1N1 influenza virus. The development of novel therapeutics or prophylactics for influenza virus infection is urgently needed. We examined the evaluation of the anti-influenza virus (A/WSN/33 (H1N1)) activity of Brazilian green propolis water extract (PWE) and its constituents by cell viability and real-time PCR assays. Our findings showed strong evidence that PWE has an anti-influenza effect and demonstrate that caffeoylquinic acids are the active anti-influenza components of PWE. Furthermore, we have found that the amount of viral RNA per cell remained unchanged even in the presence of PWE, suggesting that PWE has no direct impact on the influenza virus but may have a cytoprotective activity by affecting internal cellular process. These findings indicate that caffeoylquinic acids are the active anti-influenza components of PWE. Above findings might facilitate the prophylactic application of natural products and the realization of novel anti-influenza drugs based on caffeoylquinic acids, as well as further the understanding of cytoprotective intracellular mechanisms in influenza virus-infected cells. |
format | Text |
id | pubmed-3057164 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-30571642011-03-21 Caffeoylquinic Acids Are Major Constituents with Potent Anti-Influenza Effects in Brazilian Green Propolis Water Extract Urushisaki, Tomohiko Takemura, Tomoaki Tazawa, Shigemi Fukuoka, Mayuko Hosokawa-Muto, Junji Araki, Yoko Kuwata, Kazuo Evid Based Complement Alternat Med Research Article Influenza A viral infections reached pandemic levels in 1918, 1957, 1968, and, most recently, in 2009 with the emergence of the swine-origin H1N1 influenza virus. The development of novel therapeutics or prophylactics for influenza virus infection is urgently needed. We examined the evaluation of the anti-influenza virus (A/WSN/33 (H1N1)) activity of Brazilian green propolis water extract (PWE) and its constituents by cell viability and real-time PCR assays. Our findings showed strong evidence that PWE has an anti-influenza effect and demonstrate that caffeoylquinic acids are the active anti-influenza components of PWE. Furthermore, we have found that the amount of viral RNA per cell remained unchanged even in the presence of PWE, suggesting that PWE has no direct impact on the influenza virus but may have a cytoprotective activity by affecting internal cellular process. These findings indicate that caffeoylquinic acids are the active anti-influenza components of PWE. Above findings might facilitate the prophylactic application of natural products and the realization of novel anti-influenza drugs based on caffeoylquinic acids, as well as further the understanding of cytoprotective intracellular mechanisms in influenza virus-infected cells. Hindawi Publishing Corporation 2011 2011-03-01 /pmc/articles/PMC3057164/ /pubmed/21423687 http://dx.doi.org/10.1155/2011/254914 Text en Copyright © 2011 Tomohiko Urushisaki et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Urushisaki, Tomohiko Takemura, Tomoaki Tazawa, Shigemi Fukuoka, Mayuko Hosokawa-Muto, Junji Araki, Yoko Kuwata, Kazuo Caffeoylquinic Acids Are Major Constituents with Potent Anti-Influenza Effects in Brazilian Green Propolis Water Extract |
title | Caffeoylquinic Acids Are Major Constituents with Potent Anti-Influenza Effects in Brazilian Green Propolis Water Extract |
title_full | Caffeoylquinic Acids Are Major Constituents with Potent Anti-Influenza Effects in Brazilian Green Propolis Water Extract |
title_fullStr | Caffeoylquinic Acids Are Major Constituents with Potent Anti-Influenza Effects in Brazilian Green Propolis Water Extract |
title_full_unstemmed | Caffeoylquinic Acids Are Major Constituents with Potent Anti-Influenza Effects in Brazilian Green Propolis Water Extract |
title_short | Caffeoylquinic Acids Are Major Constituents with Potent Anti-Influenza Effects in Brazilian Green Propolis Water Extract |
title_sort | caffeoylquinic acids are major constituents with potent anti-influenza effects in brazilian green propolis water extract |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057164/ https://www.ncbi.nlm.nih.gov/pubmed/21423687 http://dx.doi.org/10.1155/2011/254914 |
work_keys_str_mv | AT urushisakitomohiko caffeoylquinicacidsaremajorconstituentswithpotentantiinfluenzaeffectsinbraziliangreenpropoliswaterextract AT takemuratomoaki caffeoylquinicacidsaremajorconstituentswithpotentantiinfluenzaeffectsinbraziliangreenpropoliswaterextract AT tazawashigemi caffeoylquinicacidsaremajorconstituentswithpotentantiinfluenzaeffectsinbraziliangreenpropoliswaterextract AT fukuokamayuko caffeoylquinicacidsaremajorconstituentswithpotentantiinfluenzaeffectsinbraziliangreenpropoliswaterextract AT hosokawamutojunji caffeoylquinicacidsaremajorconstituentswithpotentantiinfluenzaeffectsinbraziliangreenpropoliswaterextract AT arakiyoko caffeoylquinicacidsaremajorconstituentswithpotentantiinfluenzaeffectsinbraziliangreenpropoliswaterextract AT kuwatakazuo caffeoylquinicacidsaremajorconstituentswithpotentantiinfluenzaeffectsinbraziliangreenpropoliswaterextract |