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Panax notoginseng Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice

Panax notoginseng (PN) is a traditional Chinese herb experimentally proven to have anti-inflammatory effects, and it is used clinically for the treatment of atherosclerosis, cerebral infarction, and cerebral ischemia. This study aimed to determine the anti-inflammatory effects of PN against bleomyci...

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Autores principales: Tsai, Kuen-Daw, Yang, Shu-Mei, Lee, Jen-Chih, Wong, Ho-Yiu, Shih, Chuen-Ming, Lin, Ting-Hui, Tseng, Min-Jen, Chen, Wei
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057514/
https://www.ncbi.nlm.nih.gov/pubmed/21423633
http://dx.doi.org/10.1155/2011/404761
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author Tsai, Kuen-Daw
Yang, Shu-Mei
Lee, Jen-Chih
Wong, Ho-Yiu
Shih, Chuen-Ming
Lin, Ting-Hui
Tseng, Min-Jen
Chen, Wei
author_facet Tsai, Kuen-Daw
Yang, Shu-Mei
Lee, Jen-Chih
Wong, Ho-Yiu
Shih, Chuen-Ming
Lin, Ting-Hui
Tseng, Min-Jen
Chen, Wei
author_sort Tsai, Kuen-Daw
collection PubMed
description Panax notoginseng (PN) is a traditional Chinese herb experimentally proven to have anti-inflammatory effects, and it is used clinically for the treatment of atherosclerosis, cerebral infarction, and cerebral ischemia. This study aimed to determine the anti-inflammatory effects of PN against bleomycin-induced pulmonary fibrosis in mice. First, in an in vitro study, culture media containing lipopolysaccharide (LPS) was used to stimulate macrophage cells (RAW 264.7 cell line). TNF-α and IL-6 levels were then determined before and after treatment with PN extract. In an animal model (C57BL/6 mice), a single dose of PN (0.5 mg/kg) was administered orally on Day 2 or Day 7 postbleomycin treatment. The results showed that TNF-α and IL-6 levels increased in the culture media of LPS-stimulated macrophage cells, and this effect was significantly inhibited in a concentration-dependent manner by PN extract. Histopathologic examination revealed that PN administered on Day 7 postbleomycin treatment significantly decreased inflammatory cell infiltrates, fibrosis scores, and TNF-α, TGF-β, IL-1β, and IL-6 levels in bronchoalveolar lavage fluid when compared with PN given on Day 2 postbleomycin treatment. These results suggest that PN administered in the early fibrotic stage can attenuate pulmonary fibrosis in an animal model of idiopathic pulmonary fibrosis.
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spelling pubmed-30575142011-03-21 Panax notoginseng Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice Tsai, Kuen-Daw Yang, Shu-Mei Lee, Jen-Chih Wong, Ho-Yiu Shih, Chuen-Ming Lin, Ting-Hui Tseng, Min-Jen Chen, Wei Evid Based Complement Alternat Med Research Article Panax notoginseng (PN) is a traditional Chinese herb experimentally proven to have anti-inflammatory effects, and it is used clinically for the treatment of atherosclerosis, cerebral infarction, and cerebral ischemia. This study aimed to determine the anti-inflammatory effects of PN against bleomycin-induced pulmonary fibrosis in mice. First, in an in vitro study, culture media containing lipopolysaccharide (LPS) was used to stimulate macrophage cells (RAW 264.7 cell line). TNF-α and IL-6 levels were then determined before and after treatment with PN extract. In an animal model (C57BL/6 mice), a single dose of PN (0.5 mg/kg) was administered orally on Day 2 or Day 7 postbleomycin treatment. The results showed that TNF-α and IL-6 levels increased in the culture media of LPS-stimulated macrophage cells, and this effect was significantly inhibited in a concentration-dependent manner by PN extract. Histopathologic examination revealed that PN administered on Day 7 postbleomycin treatment significantly decreased inflammatory cell infiltrates, fibrosis scores, and TNF-α, TGF-β, IL-1β, and IL-6 levels in bronchoalveolar lavage fluid when compared with PN given on Day 2 postbleomycin treatment. These results suggest that PN administered in the early fibrotic stage can attenuate pulmonary fibrosis in an animal model of idiopathic pulmonary fibrosis. Hindawi Publishing Corporation 2011 2011-03-06 /pmc/articles/PMC3057514/ /pubmed/21423633 http://dx.doi.org/10.1155/2011/404761 Text en Copyright © 2011 Kuen-Daw Tsai et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tsai, Kuen-Daw
Yang, Shu-Mei
Lee, Jen-Chih
Wong, Ho-Yiu
Shih, Chuen-Ming
Lin, Ting-Hui
Tseng, Min-Jen
Chen, Wei
Panax notoginseng Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice
title Panax notoginseng Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice
title_full Panax notoginseng Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice
title_fullStr Panax notoginseng Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice
title_full_unstemmed Panax notoginseng Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice
title_short Panax notoginseng Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice
title_sort panax notoginseng attenuates bleomycin-induced pulmonary fibrosis in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057514/
https://www.ncbi.nlm.nih.gov/pubmed/21423633
http://dx.doi.org/10.1155/2011/404761
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