Cargando…

Calcium/calmodulin kinase II activity of hippocampus in kainate-induced epilepsy.

This study investigated calcium/calmodulin kinase II (CaMKII) activity related to long-standing neuronal injury of the hippocampus in kainate (KA)-induced experimental temporal lobe epilepsy. Epileptic seizure was induced by injection of KA (1 microg/microL) dissolved in phosphate buffer (0.1 M, pH...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, M. C., Ban, S. S., Woo, Y. J., Kim, S. U.
Formato: Texto
Lenguaje:English
Publicado: Korean Academy of Medical Sciences 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057582/
https://www.ncbi.nlm.nih.gov/pubmed/11641537
_version_ 1782200292231610368
author Lee, M. C.
Ban, S. S.
Woo, Y. J.
Kim, S. U.
author_facet Lee, M. C.
Ban, S. S.
Woo, Y. J.
Kim, S. U.
author_sort Lee, M. C.
collection PubMed
description This study investigated calcium/calmodulin kinase II (CaMKII) activity related to long-standing neuronal injury of the hippocampus in kainate (KA)-induced experimental temporal lobe epilepsy. Epileptic seizure was induced by injection of KA (1 microg/microL) dissolved in phosphate buffer (0.1 M, pH 7.4) into the left amygdala. Clinical seizures, histopathologic changes and CaMKII activity of the hippocampus were evaluated. Characteristic early limbic and late seizures were developed. Hippocampal CaMKII activity increased significantly 4 and 8 weeks after intra-amygdaloid injection of KA, when late seizures developed. The histopathologic changes of the hippocampus included swelling of neuronal cytoplasm with nuclear pyknosis and loss of neurons in CA3 during this period. The increased activity of CaMKII may correlate with appearance of distant damage in the hippocampus. The above results indicate that intra-amygdaloid injection of KA produces excitatory signals for ipsilateral CA3 neurons in the hippocampus and that subsequently increased levels of CaMKII in postsynaptic neurons induce neuronal injury via phosphorylation of N-methyl-D-aspartate type glutamate receptor.
format Text
id pubmed-3057582
institution National Center for Biotechnology Information
language English
publishDate 2001
publisher Korean Academy of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-30575822011-03-31 Calcium/calmodulin kinase II activity of hippocampus in kainate-induced epilepsy. Lee, M. C. Ban, S. S. Woo, Y. J. Kim, S. U. J Korean Med Sci Research Article This study investigated calcium/calmodulin kinase II (CaMKII) activity related to long-standing neuronal injury of the hippocampus in kainate (KA)-induced experimental temporal lobe epilepsy. Epileptic seizure was induced by injection of KA (1 microg/microL) dissolved in phosphate buffer (0.1 M, pH 7.4) into the left amygdala. Clinical seizures, histopathologic changes and CaMKII activity of the hippocampus were evaluated. Characteristic early limbic and late seizures were developed. Hippocampal CaMKII activity increased significantly 4 and 8 weeks after intra-amygdaloid injection of KA, when late seizures developed. The histopathologic changes of the hippocampus included swelling of neuronal cytoplasm with nuclear pyknosis and loss of neurons in CA3 during this period. The increased activity of CaMKII may correlate with appearance of distant damage in the hippocampus. The above results indicate that intra-amygdaloid injection of KA produces excitatory signals for ipsilateral CA3 neurons in the hippocampus and that subsequently increased levels of CaMKII in postsynaptic neurons induce neuronal injury via phosphorylation of N-methyl-D-aspartate type glutamate receptor. Korean Academy of Medical Sciences 2001-10 /pmc/articles/PMC3057582/ /pubmed/11641537 Text en
spellingShingle Research Article
Lee, M. C.
Ban, S. S.
Woo, Y. J.
Kim, S. U.
Calcium/calmodulin kinase II activity of hippocampus in kainate-induced epilepsy.
title Calcium/calmodulin kinase II activity of hippocampus in kainate-induced epilepsy.
title_full Calcium/calmodulin kinase II activity of hippocampus in kainate-induced epilepsy.
title_fullStr Calcium/calmodulin kinase II activity of hippocampus in kainate-induced epilepsy.
title_full_unstemmed Calcium/calmodulin kinase II activity of hippocampus in kainate-induced epilepsy.
title_short Calcium/calmodulin kinase II activity of hippocampus in kainate-induced epilepsy.
title_sort calcium/calmodulin kinase ii activity of hippocampus in kainate-induced epilepsy.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057582/
https://www.ncbi.nlm.nih.gov/pubmed/11641537
work_keys_str_mv AT leemc calciumcalmodulinkinaseiiactivityofhippocampusinkainateinducedepilepsy
AT banss calciumcalmodulinkinaseiiactivityofhippocampusinkainateinducedepilepsy
AT wooyj calciumcalmodulinkinaseiiactivityofhippocampusinkainateinducedepilepsy
AT kimsu calciumcalmodulinkinaseiiactivityofhippocampusinkainateinducedepilepsy