Cargando…
Holographic opto-fluidic microscopy
Over the last decade microfluidics has created a versatile platform that has significantly advanced the ways in which micro-scale organisms and objects are controlled, processed and investigated, by improving the cost, compactness and throughput aspects of analysis. Microfluidics has also expanded i...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057885/ https://www.ncbi.nlm.nih.gov/pubmed/21197025 http://dx.doi.org/10.1364/OE.18.027499 |
_version_ | 1782200309376876544 |
---|---|
author | Bishara, Waheb Zhu, Hongying Ozcan, Aydogan |
author_facet | Bishara, Waheb Zhu, Hongying Ozcan, Aydogan |
author_sort | Bishara, Waheb |
collection | PubMed |
description | Over the last decade microfluidics has created a versatile platform that has significantly advanced the ways in which micro-scale organisms and objects are controlled, processed and investigated, by improving the cost, compactness and throughput aspects of analysis. Microfluidics has also expanded into optics to create reconfigurable and flexible optical devices such as reconfigurable lenses, lasers, waveguides, switches, and on-chip microscopes. Here we present a new opto-fluidic microscopy modality, i.e., Holographic Opto-fluidic Microscopy (HOM), based on lensless holographic imaging. This imaging modality complements the miniaturization provided by microfluidics and would allow the integration of microscopy into existing on-chip microfluidic devices with various functionalities. Our imaging modality utilizes partially coherent in-line holography and pixel super-resolution to create high-resolution amplitude and phase images of the objects flowing within micro-fluidic channels, which we demonstrate by imaging C. elegans, Giardia lamblia, and Mulberry pollen. HOM does not involve complicated fabrication processes or precise alignment, nor does it require a highly uniform flow of objects within microfluidic channels. |
format | Text |
id | pubmed-3057885 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-30578852011-03-15 Holographic opto-fluidic microscopy Bishara, Waheb Zhu, Hongying Ozcan, Aydogan Opt Express Research-Article Over the last decade microfluidics has created a versatile platform that has significantly advanced the ways in which micro-scale organisms and objects are controlled, processed and investigated, by improving the cost, compactness and throughput aspects of analysis. Microfluidics has also expanded into optics to create reconfigurable and flexible optical devices such as reconfigurable lenses, lasers, waveguides, switches, and on-chip microscopes. Here we present a new opto-fluidic microscopy modality, i.e., Holographic Opto-fluidic Microscopy (HOM), based on lensless holographic imaging. This imaging modality complements the miniaturization provided by microfluidics and would allow the integration of microscopy into existing on-chip microfluidic devices with various functionalities. Our imaging modality utilizes partially coherent in-line holography and pixel super-resolution to create high-resolution amplitude and phase images of the objects flowing within micro-fluidic channels, which we demonstrate by imaging C. elegans, Giardia lamblia, and Mulberry pollen. HOM does not involve complicated fabrication processes or precise alignment, nor does it require a highly uniform flow of objects within microfluidic channels. Optical Society of America 2010-12-14 /pmc/articles/PMC3057885/ /pubmed/21197025 http://dx.doi.org/10.1364/OE.18.027499 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Research-Article Bishara, Waheb Zhu, Hongying Ozcan, Aydogan Holographic opto-fluidic microscopy |
title | Holographic opto-fluidic microscopy |
title_full | Holographic opto-fluidic microscopy |
title_fullStr | Holographic opto-fluidic microscopy |
title_full_unstemmed | Holographic opto-fluidic microscopy |
title_short | Holographic opto-fluidic microscopy |
title_sort | holographic opto-fluidic microscopy |
topic | Research-Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057885/ https://www.ncbi.nlm.nih.gov/pubmed/21197025 http://dx.doi.org/10.1364/OE.18.027499 |
work_keys_str_mv | AT bisharawaheb holographicoptofluidicmicroscopy AT zhuhongying holographicoptofluidicmicroscopy AT ozcanaydogan holographicoptofluidicmicroscopy |