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A modified scoring system to describe gross pathology in the rabbit model of tuberculosis

BACKGROUND: The rabbit model is an ideal means to study the pathogenesis of tuberculosis due to its semblance to the disease in humans. We have previously described the results using a bronchoscopic route of infection with live bacilli as a reliable means of generating lung cavities in sensitized ra...

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Autores principales: Jassal, Mandeep S, Nedeltchev, Gueno G, Osborne, Jonathan, Bishai, William R
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058006/
https://www.ncbi.nlm.nih.gov/pubmed/21375756
http://dx.doi.org/10.1186/1471-2180-11-49
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author Jassal, Mandeep S
Nedeltchev, Gueno G
Osborne, Jonathan
Bishai, William R
author_facet Jassal, Mandeep S
Nedeltchev, Gueno G
Osborne, Jonathan
Bishai, William R
author_sort Jassal, Mandeep S
collection PubMed
description BACKGROUND: The rabbit model is an ideal means to study the pathogenesis of tuberculosis due to its semblance to the disease in humans. We have previously described the results using a bronchoscopic route of infection with live bacilli as a reliable means of generating lung cavities in sensitized rabbits. The role of sensitization in the development of disease outcomes has been well established in several animal models. We have described here the varying gross pathology that result from lack of sensitization with heat-killed M. bovis prior to high-dose bronchoscopic infection with live bacilli. RESULTS: Rabbits lacking sensitization did not generate lung cavities, but instead formed solely a tuberculoid pneumonia that replaced the normal lung parenchyma in the area of infection. Extrapulmonary dissemination was seen in approximately equal frequency and distribution in both rabbit populations. Notable differences include the lack of intestinal lesions in non-sensitized rabbits likely due to the lack of ingestion of expectorated bacilli from cavitary lesions. The experiment also employed a modified scoring system developed initially in the primate model of tuberculosis to allow for the quantification of findings observed at necropsy. CONCLUSIONS: To date, no such scoring system has been employed in the rabbit model to describe gross pathology. The quantitative methodology would allow for rapid comparative analyses and standardization of thoracic and extrapulmonary pathology that could be evaluated for statistical significance. The aim is to use such a scoring system as the foundation for all future rabbit studies describing gross pathology at all stages in TB pathogenesis.
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spelling pubmed-30580062011-03-16 A modified scoring system to describe gross pathology in the rabbit model of tuberculosis Jassal, Mandeep S Nedeltchev, Gueno G Osborne, Jonathan Bishai, William R BMC Microbiol Research Article BACKGROUND: The rabbit model is an ideal means to study the pathogenesis of tuberculosis due to its semblance to the disease in humans. We have previously described the results using a bronchoscopic route of infection with live bacilli as a reliable means of generating lung cavities in sensitized rabbits. The role of sensitization in the development of disease outcomes has been well established in several animal models. We have described here the varying gross pathology that result from lack of sensitization with heat-killed M. bovis prior to high-dose bronchoscopic infection with live bacilli. RESULTS: Rabbits lacking sensitization did not generate lung cavities, but instead formed solely a tuberculoid pneumonia that replaced the normal lung parenchyma in the area of infection. Extrapulmonary dissemination was seen in approximately equal frequency and distribution in both rabbit populations. Notable differences include the lack of intestinal lesions in non-sensitized rabbits likely due to the lack of ingestion of expectorated bacilli from cavitary lesions. The experiment also employed a modified scoring system developed initially in the primate model of tuberculosis to allow for the quantification of findings observed at necropsy. CONCLUSIONS: To date, no such scoring system has been employed in the rabbit model to describe gross pathology. The quantitative methodology would allow for rapid comparative analyses and standardization of thoracic and extrapulmonary pathology that could be evaluated for statistical significance. The aim is to use such a scoring system as the foundation for all future rabbit studies describing gross pathology at all stages in TB pathogenesis. BioMed Central 2011-03-04 /pmc/articles/PMC3058006/ /pubmed/21375756 http://dx.doi.org/10.1186/1471-2180-11-49 Text en Copyright ©2011 Jassal et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jassal, Mandeep S
Nedeltchev, Gueno G
Osborne, Jonathan
Bishai, William R
A modified scoring system to describe gross pathology in the rabbit model of tuberculosis
title A modified scoring system to describe gross pathology in the rabbit model of tuberculosis
title_full A modified scoring system to describe gross pathology in the rabbit model of tuberculosis
title_fullStr A modified scoring system to describe gross pathology in the rabbit model of tuberculosis
title_full_unstemmed A modified scoring system to describe gross pathology in the rabbit model of tuberculosis
title_short A modified scoring system to describe gross pathology in the rabbit model of tuberculosis
title_sort modified scoring system to describe gross pathology in the rabbit model of tuberculosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058006/
https://www.ncbi.nlm.nih.gov/pubmed/21375756
http://dx.doi.org/10.1186/1471-2180-11-49
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