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Dispensing pico to nanolitre of a natural hydrogel by laser-assisted bioprinting

BACKGROUND: Laser-assisted bioprinting of multi-cellular replicates in accordance with CAD blueprint may substantially improve our understandings of fundamental aspects of 3 D cell-cell and cell-matrix interactions in vitro. For predictable printing results, a profound knowledge about effects of dif...

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Detalles Bibliográficos
Autores principales: Gruene, Martin, Unger, Claudia, Koch, Lothar, Deiwick, Andrea, Chichkov, Boris
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058070/
https://www.ncbi.nlm.nih.gov/pubmed/21385332
http://dx.doi.org/10.1186/1475-925X-10-19
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author Gruene, Martin
Unger, Claudia
Koch, Lothar
Deiwick, Andrea
Chichkov, Boris
author_facet Gruene, Martin
Unger, Claudia
Koch, Lothar
Deiwick, Andrea
Chichkov, Boris
author_sort Gruene, Martin
collection PubMed
description BACKGROUND: Laser-assisted bioprinting of multi-cellular replicates in accordance with CAD blueprint may substantially improve our understandings of fundamental aspects of 3 D cell-cell and cell-matrix interactions in vitro. For predictable printing results, a profound knowledge about effects of different processing parameters is essential for realisation of 3 D cell models with well-defined cell densities. METHODS: Time-resolved imaging of the hydrogel jet dynamics and quantitative assessment of the dependence of printed droplet diameter on the process characteristics were conducted. RESULTS: The existence of a counterjet was visualised, proving the bubble collapsing theory for the jet formation. Furthermore, by adjusting the viscosity and height of the applied hydrogel layer in combination with different laser pulse energies, the printing of volumes in the range of 10 to 7000 picolitres was demonstrated. Additionally, the relationship between the viscosity and the layer thickness at different laser pulse energies on the printed droplet volume was identified. CONCLUSIONS: These findings are essential for the advancement of laser-assisted bioprinting by enabling predictable printing results and the integration of computational methods in the generation of 3 D multi-cellular constructs.
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spelling pubmed-30580702011-03-16 Dispensing pico to nanolitre of a natural hydrogel by laser-assisted bioprinting Gruene, Martin Unger, Claudia Koch, Lothar Deiwick, Andrea Chichkov, Boris Biomed Eng Online Research BACKGROUND: Laser-assisted bioprinting of multi-cellular replicates in accordance with CAD blueprint may substantially improve our understandings of fundamental aspects of 3 D cell-cell and cell-matrix interactions in vitro. For predictable printing results, a profound knowledge about effects of different processing parameters is essential for realisation of 3 D cell models with well-defined cell densities. METHODS: Time-resolved imaging of the hydrogel jet dynamics and quantitative assessment of the dependence of printed droplet diameter on the process characteristics were conducted. RESULTS: The existence of a counterjet was visualised, proving the bubble collapsing theory for the jet formation. Furthermore, by adjusting the viscosity and height of the applied hydrogel layer in combination with different laser pulse energies, the printing of volumes in the range of 10 to 7000 picolitres was demonstrated. Additionally, the relationship between the viscosity and the layer thickness at different laser pulse energies on the printed droplet volume was identified. CONCLUSIONS: These findings are essential for the advancement of laser-assisted bioprinting by enabling predictable printing results and the integration of computational methods in the generation of 3 D multi-cellular constructs. BioMed Central 2011-03-07 /pmc/articles/PMC3058070/ /pubmed/21385332 http://dx.doi.org/10.1186/1475-925X-10-19 Text en Copyright ©2011 Gruene et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Gruene, Martin
Unger, Claudia
Koch, Lothar
Deiwick, Andrea
Chichkov, Boris
Dispensing pico to nanolitre of a natural hydrogel by laser-assisted bioprinting
title Dispensing pico to nanolitre of a natural hydrogel by laser-assisted bioprinting
title_full Dispensing pico to nanolitre of a natural hydrogel by laser-assisted bioprinting
title_fullStr Dispensing pico to nanolitre of a natural hydrogel by laser-assisted bioprinting
title_full_unstemmed Dispensing pico to nanolitre of a natural hydrogel by laser-assisted bioprinting
title_short Dispensing pico to nanolitre of a natural hydrogel by laser-assisted bioprinting
title_sort dispensing pico to nanolitre of a natural hydrogel by laser-assisted bioprinting
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058070/
https://www.ncbi.nlm.nih.gov/pubmed/21385332
http://dx.doi.org/10.1186/1475-925X-10-19
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