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Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs
An outbreak of echovirus 5 (ECV 5) occurred in Korea in 2006, marking the first time this virus had been identified in the country since enterovirus surveillance began in 1993. Using a sample isolated from a young male patient with aseptic meningitis, we performed sequencing of the Korean ECV 5 stra...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058091/ https://www.ncbi.nlm.nih.gov/pubmed/21345236 http://dx.doi.org/10.1186/1743-422X-8-79 |
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author | Park, Kwisung Song, Jaehyoung Baek, Kyoungah Lee, Changgyun Kim, Donguk Cho, SamHyun Park, JoonSoo Choi, YoungJin Kang, Byunghak Choi, Hwajung Cheon, Doo-Sung |
author_facet | Park, Kwisung Song, Jaehyoung Baek, Kyoungah Lee, Changgyun Kim, Donguk Cho, SamHyun Park, JoonSoo Choi, YoungJin Kang, Byunghak Choi, Hwajung Cheon, Doo-Sung |
author_sort | Park, Kwisung |
collection | PubMed |
description | An outbreak of echovirus 5 (ECV 5) occurred in Korea in 2006, marking the first time this virus had been identified in the country since enterovirus surveillance began in 1993. Using a sample isolated from a young male patient with aseptic meningitis, we performed sequencing of the Korean ECV 5 strain and compared it with a prototype strain (Noyce). At the nucleotide level, the P1 region (85.3%) had the highest identity value; at the amino acid level, the P3 region (98.0%) had the highest identity value. The two strains shared all cleavage sites, with the exception of the VP1/2A site, which was TY/GA in the Noyce strain but TR/GA in the Korean ECV 5 isolate. In Vero cells infected with the Korean ECV 5 isolate, no cytotoxicity was observed in the presence of azidothymidine, acyclovir, amantadine, lamivudine, or ribavirin, when the drugs were administered at a CC(50 )value >100 μg/mL. Of the five drugs, only amantadine (IC(50): 1 ± 0.42 μg/mL, TI: 100) and ribavirin (IC(50): 22 ± 1.36 μg/mL, TI: 4.55) had any antiviral activity against the Korean ECV 5 isolate. |
format | Text |
id | pubmed-3058091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30580912011-03-18 Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs Park, Kwisung Song, Jaehyoung Baek, Kyoungah Lee, Changgyun Kim, Donguk Cho, SamHyun Park, JoonSoo Choi, YoungJin Kang, Byunghak Choi, Hwajung Cheon, Doo-Sung Virol J Short Report An outbreak of echovirus 5 (ECV 5) occurred in Korea in 2006, marking the first time this virus had been identified in the country since enterovirus surveillance began in 1993. Using a sample isolated from a young male patient with aseptic meningitis, we performed sequencing of the Korean ECV 5 strain and compared it with a prototype strain (Noyce). At the nucleotide level, the P1 region (85.3%) had the highest identity value; at the amino acid level, the P3 region (98.0%) had the highest identity value. The two strains shared all cleavage sites, with the exception of the VP1/2A site, which was TY/GA in the Noyce strain but TR/GA in the Korean ECV 5 isolate. In Vero cells infected with the Korean ECV 5 isolate, no cytotoxicity was observed in the presence of azidothymidine, acyclovir, amantadine, lamivudine, or ribavirin, when the drugs were administered at a CC(50 )value >100 μg/mL. Of the five drugs, only amantadine (IC(50): 1 ± 0.42 μg/mL, TI: 100) and ribavirin (IC(50): 22 ± 1.36 μg/mL, TI: 4.55) had any antiviral activity against the Korean ECV 5 isolate. BioMed Central 2011-02-24 /pmc/articles/PMC3058091/ /pubmed/21345236 http://dx.doi.org/10.1186/1743-422X-8-79 Text en Copyright ©2011 Park et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Park, Kwisung Song, Jaehyoung Baek, Kyoungah Lee, Changgyun Kim, Donguk Cho, SamHyun Park, JoonSoo Choi, YoungJin Kang, Byunghak Choi, Hwajung Cheon, Doo-Sung Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs |
title | Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs |
title_full | Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs |
title_fullStr | Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs |
title_full_unstemmed | Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs |
title_short | Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs |
title_sort | genetic diversity of a korean echovirus 5 isolate and response of the strain to five antiviral drugs |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058091/ https://www.ncbi.nlm.nih.gov/pubmed/21345236 http://dx.doi.org/10.1186/1743-422X-8-79 |
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