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Occult HBV infection among Egyptian hepatocellular carcinoma patients
BACKGROUND: Occult HBV infection accelerates the progression of liver fibrosis, cirrhosis, and finally leading to hepatocellular carcinoma (HCC). This study analyzed the occult HBV-genotypes in HCC patients. METHODS: To achieve our objective, matched serum and tissue samples were collected from 40 H...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058093/ https://www.ncbi.nlm.nih.gov/pubmed/21371325 http://dx.doi.org/10.1186/1743-422X-8-90 |
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author | Hassan, Zeinab K Hafez, Mohamed M Mansor, Tarek M Zekri, Abdel Rahman N |
author_facet | Hassan, Zeinab K Hafez, Mohamed M Mansor, Tarek M Zekri, Abdel Rahman N |
author_sort | Hassan, Zeinab K |
collection | PubMed |
description | BACKGROUND: Occult HBV infection accelerates the progression of liver fibrosis, cirrhosis, and finally leading to hepatocellular carcinoma (HCC). This study analyzed the occult HBV-genotypes in HCC patients. METHODS: To achieve our objective, matched serum and tissue samples were collected from 40 HCC patients. Three sets of primers were used for the HBV-DNA detection by nested-PCR, which cover the HBV-genome; Core, Surface and X genes. Genotyping system based on PCR using type-specific primers was applied on HBV-DNA positive samples. RESULTS: Intrahepatic occult HBV-DNA was detected in 62.5%, whereas; Serum occult HBV-DNA were detected in only 22.5% of HCC patients. In patients' positive for both anti-HBs and anti-HBc, 10% had occult HBV in serum. In serologically negative HCV patients, 63% had intrahepatic HBV-DNA, and 21% had HBV-DNA in serum samples. HBV-genotype D (32%) and B (24%) attributed predominantly to intrahepatic HBV infections in HCC patients, whereas HBV-genotype A (4%) and C (8%) infections were the least observed. CONCLUSION: This is the first study to show the genotypes of occult HBV infection in HCC Patients. We suggest that B or D may influence the outcome of HBV infection which may lead to the development of HCC. |
format | Text |
id | pubmed-3058093 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30580932011-03-16 Occult HBV infection among Egyptian hepatocellular carcinoma patients Hassan, Zeinab K Hafez, Mohamed M Mansor, Tarek M Zekri, Abdel Rahman N Virol J Research BACKGROUND: Occult HBV infection accelerates the progression of liver fibrosis, cirrhosis, and finally leading to hepatocellular carcinoma (HCC). This study analyzed the occult HBV-genotypes in HCC patients. METHODS: To achieve our objective, matched serum and tissue samples were collected from 40 HCC patients. Three sets of primers were used for the HBV-DNA detection by nested-PCR, which cover the HBV-genome; Core, Surface and X genes. Genotyping system based on PCR using type-specific primers was applied on HBV-DNA positive samples. RESULTS: Intrahepatic occult HBV-DNA was detected in 62.5%, whereas; Serum occult HBV-DNA were detected in only 22.5% of HCC patients. In patients' positive for both anti-HBs and anti-HBc, 10% had occult HBV in serum. In serologically negative HCV patients, 63% had intrahepatic HBV-DNA, and 21% had HBV-DNA in serum samples. HBV-genotype D (32%) and B (24%) attributed predominantly to intrahepatic HBV infections in HCC patients, whereas HBV-genotype A (4%) and C (8%) infections were the least observed. CONCLUSION: This is the first study to show the genotypes of occult HBV infection in HCC Patients. We suggest that B or D may influence the outcome of HBV infection which may lead to the development of HCC. BioMed Central 2011-03-03 /pmc/articles/PMC3058093/ /pubmed/21371325 http://dx.doi.org/10.1186/1743-422X-8-90 Text en Copyright ©2011 Hassan et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Hassan, Zeinab K Hafez, Mohamed M Mansor, Tarek M Zekri, Abdel Rahman N Occult HBV infection among Egyptian hepatocellular carcinoma patients |
title | Occult HBV infection among Egyptian hepatocellular carcinoma patients |
title_full | Occult HBV infection among Egyptian hepatocellular carcinoma patients |
title_fullStr | Occult HBV infection among Egyptian hepatocellular carcinoma patients |
title_full_unstemmed | Occult HBV infection among Egyptian hepatocellular carcinoma patients |
title_short | Occult HBV infection among Egyptian hepatocellular carcinoma patients |
title_sort | occult hbv infection among egyptian hepatocellular carcinoma patients |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058093/ https://www.ncbi.nlm.nih.gov/pubmed/21371325 http://dx.doi.org/10.1186/1743-422X-8-90 |
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