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Glibenclamide increases post-fatigue tension in slow skeletal muscle fibers of the chicken

In contrast to fast-twitch skeletal muscle fibers of the chicken, slow-twitch fibers are fatigue-resistant. In fast fibers, the fatigue process has been related to K(ATP) channels. In the present study, we investigated the action of glibenclamide (an anti-diabetic sulphonylurea that acts on K(ATP) c...

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Autores principales: Andrade, Felipa, Trujillo, Xóchitl, Sánchez-Pastor, Enrique, Montoya-Pérez, Rocío, Saavedra-Molina, Alfredo, Ortiz-Mesina, Mónica, Huerta, Miguel
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058534/
https://www.ncbi.nlm.nih.gov/pubmed/21079972
http://dx.doi.org/10.1007/s00360-010-0527-1
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author Andrade, Felipa
Trujillo, Xóchitl
Sánchez-Pastor, Enrique
Montoya-Pérez, Rocío
Saavedra-Molina, Alfredo
Ortiz-Mesina, Mónica
Huerta, Miguel
author_facet Andrade, Felipa
Trujillo, Xóchitl
Sánchez-Pastor, Enrique
Montoya-Pérez, Rocío
Saavedra-Molina, Alfredo
Ortiz-Mesina, Mónica
Huerta, Miguel
author_sort Andrade, Felipa
collection PubMed
description In contrast to fast-twitch skeletal muscle fibers of the chicken, slow-twitch fibers are fatigue-resistant. In fast fibers, the fatigue process has been related to K(ATP) channels. In the present study, we investigated the action of glibenclamide (an anti-diabetic sulphonylurea that acts on K(ATP) channels) on fatigued slow skeletal muscle, studying twitch and tetanus tension after inducing the muscle to fatigue by continuous electrical stimulation. Our results showed that glibenclamide (150 μM) increased post-fatigue twitch tension by about 25% with respect to the fatigued condition (P < 0.05). In addition, glibenclamide (150 μM) increased post-fatigue tetanic tension (83.61 ± 15.7% in peak tension, and 85.0 ± 19.0% in tension-time integral, P = 0.02, and 0.04, respectively; n = 3). Moreover, after exposing the muscle to a condition that inhibits mitochondrial ATP formation in order to activate K(ATP) channels with cyanide (10 mM), tension also diminished, but in the presence of glibenclamide the effect produced by cyanide was abolished. To determine a possible increase in intracellular calcium concentration, the effects of glibenclamide on caffeine-evoked contractures were explored. After muscle pre-incubation with glibenclamide (150 μM), tension of caffeine-evoked contractures increased (6.5 ± 1.5% in maximal tension, and 5.9 ± 3.8% in tension-time integral, P < 0.05). These results suggest a possible role of K(ATP) channels in the fatigue process, since glibenclamide increases twitch and tetanus tension in fatigued slow muscle of the chicken and during metabolic inhibition, possibly by increasing intracellular calcium.
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spelling pubmed-30585342011-04-05 Glibenclamide increases post-fatigue tension in slow skeletal muscle fibers of the chicken Andrade, Felipa Trujillo, Xóchitl Sánchez-Pastor, Enrique Montoya-Pérez, Rocío Saavedra-Molina, Alfredo Ortiz-Mesina, Mónica Huerta, Miguel J Comp Physiol B Original Paper In contrast to fast-twitch skeletal muscle fibers of the chicken, slow-twitch fibers are fatigue-resistant. In fast fibers, the fatigue process has been related to K(ATP) channels. In the present study, we investigated the action of glibenclamide (an anti-diabetic sulphonylurea that acts on K(ATP) channels) on fatigued slow skeletal muscle, studying twitch and tetanus tension after inducing the muscle to fatigue by continuous electrical stimulation. Our results showed that glibenclamide (150 μM) increased post-fatigue twitch tension by about 25% with respect to the fatigued condition (P < 0.05). In addition, glibenclamide (150 μM) increased post-fatigue tetanic tension (83.61 ± 15.7% in peak tension, and 85.0 ± 19.0% in tension-time integral, P = 0.02, and 0.04, respectively; n = 3). Moreover, after exposing the muscle to a condition that inhibits mitochondrial ATP formation in order to activate K(ATP) channels with cyanide (10 mM), tension also diminished, but in the presence of glibenclamide the effect produced by cyanide was abolished. To determine a possible increase in intracellular calcium concentration, the effects of glibenclamide on caffeine-evoked contractures were explored. After muscle pre-incubation with glibenclamide (150 μM), tension of caffeine-evoked contractures increased (6.5 ± 1.5% in maximal tension, and 5.9 ± 3.8% in tension-time integral, P < 0.05). These results suggest a possible role of K(ATP) channels in the fatigue process, since glibenclamide increases twitch and tetanus tension in fatigued slow muscle of the chicken and during metabolic inhibition, possibly by increasing intracellular calcium. Springer-Verlag 2010-11-16 2011 /pmc/articles/PMC3058534/ /pubmed/21079972 http://dx.doi.org/10.1007/s00360-010-0527-1 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Paper
Andrade, Felipa
Trujillo, Xóchitl
Sánchez-Pastor, Enrique
Montoya-Pérez, Rocío
Saavedra-Molina, Alfredo
Ortiz-Mesina, Mónica
Huerta, Miguel
Glibenclamide increases post-fatigue tension in slow skeletal muscle fibers of the chicken
title Glibenclamide increases post-fatigue tension in slow skeletal muscle fibers of the chicken
title_full Glibenclamide increases post-fatigue tension in slow skeletal muscle fibers of the chicken
title_fullStr Glibenclamide increases post-fatigue tension in slow skeletal muscle fibers of the chicken
title_full_unstemmed Glibenclamide increases post-fatigue tension in slow skeletal muscle fibers of the chicken
title_short Glibenclamide increases post-fatigue tension in slow skeletal muscle fibers of the chicken
title_sort glibenclamide increases post-fatigue tension in slow skeletal muscle fibers of the chicken
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058534/
https://www.ncbi.nlm.nih.gov/pubmed/21079972
http://dx.doi.org/10.1007/s00360-010-0527-1
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