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A widespread family of polymorphic contact-dependent toxin delivery systems in bacteria

Bacteria have developed mechanisms to communicate and compete with one another in diverse environments 1. A new form of intercellular communication, contact-dependent growth inhibition (CDI), was discovered recently in Escherichia coli 2. CDI is mediated by the CdiB/CdiA two-partner secretion system...

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Autores principales: Aoki, Stephanie K., Diner, Elie J., de Roodenbeke, Claire t'Kint, Burgess, Brandt R., Poole, Stephen J., Braaten, Bruce A., Jones, Allison M., Webb, Julia S., Hayes, Christopher S., Cotter, Peggy A., Low, David A.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058911/
https://www.ncbi.nlm.nih.gov/pubmed/21085179
http://dx.doi.org/10.1038/nature09490
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author Aoki, Stephanie K.
Diner, Elie J.
de Roodenbeke, Claire t'Kint
Burgess, Brandt R.
Poole, Stephen J.
Braaten, Bruce A.
Jones, Allison M.
Webb, Julia S.
Hayes, Christopher S.
Cotter, Peggy A.
Low, David A.
author_facet Aoki, Stephanie K.
Diner, Elie J.
de Roodenbeke, Claire t'Kint
Burgess, Brandt R.
Poole, Stephen J.
Braaten, Bruce A.
Jones, Allison M.
Webb, Julia S.
Hayes, Christopher S.
Cotter, Peggy A.
Low, David A.
author_sort Aoki, Stephanie K.
collection PubMed
description Bacteria have developed mechanisms to communicate and compete with one another in diverse environments 1. A new form of intercellular communication, contact-dependent growth inhibition (CDI), was discovered recently in Escherichia coli 2. CDI is mediated by the CdiB/CdiA two-partner secretion system. CdiB facilitates secretion of the CdiA ‘exoprotein’ onto the cell surface. An additional immunity protein (CdiI) protects CDI(+) cells from autoinhibition 2, 3. The mechanisms by which CDI blocks cell growth and CdiI counteracts this growth arrest are unknown. Moreover, the existence of CDI activity in other bacteria has not been explored. Here we show that the CDI growth inhibitory activity resides within the carboxy-terminal region of CdiA (CdiA-CT), and that CdiI binds and inactivates cognate CdiA-CT, but not heterologous CdiA-CT. Bioinformatic and experimental analyses show that multiple bacterial species encode functional CDI systems with high sequence variability in the CdiA-CT and CdiI coding regions. CdiA-CT heterogeneity implies that a range of toxic activities are utilized during CDI. Indeed, CdiA-CTs from uropathogenic E. coli and the plant pathogen Dickeya dadantii have different nuclease activities, each providing a distinct mechanism of growth inhibition. Finally, we show that bacteria lacking the CdiA-CT and CdiI coding regions are unable to compete with isogenic wild-type CDI(+) cells in both laboratory media and upon a eukaryotic host. Taken together, these results suggest that CDI systems constitute an intricate immunity network that plays an important role in bacterial competition.
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spelling pubmed-30589112011-05-18 A widespread family of polymorphic contact-dependent toxin delivery systems in bacteria Aoki, Stephanie K. Diner, Elie J. de Roodenbeke, Claire t'Kint Burgess, Brandt R. Poole, Stephen J. Braaten, Bruce A. Jones, Allison M. Webb, Julia S. Hayes, Christopher S. Cotter, Peggy A. Low, David A. Nature Article Bacteria have developed mechanisms to communicate and compete with one another in diverse environments 1. A new form of intercellular communication, contact-dependent growth inhibition (CDI), was discovered recently in Escherichia coli 2. CDI is mediated by the CdiB/CdiA two-partner secretion system. CdiB facilitates secretion of the CdiA ‘exoprotein’ onto the cell surface. An additional immunity protein (CdiI) protects CDI(+) cells from autoinhibition 2, 3. The mechanisms by which CDI blocks cell growth and CdiI counteracts this growth arrest are unknown. Moreover, the existence of CDI activity in other bacteria has not been explored. Here we show that the CDI growth inhibitory activity resides within the carboxy-terminal region of CdiA (CdiA-CT), and that CdiI binds and inactivates cognate CdiA-CT, but not heterologous CdiA-CT. Bioinformatic and experimental analyses show that multiple bacterial species encode functional CDI systems with high sequence variability in the CdiA-CT and CdiI coding regions. CdiA-CT heterogeneity implies that a range of toxic activities are utilized during CDI. Indeed, CdiA-CTs from uropathogenic E. coli and the plant pathogen Dickeya dadantii have different nuclease activities, each providing a distinct mechanism of growth inhibition. Finally, we show that bacteria lacking the CdiA-CT and CdiI coding regions are unable to compete with isogenic wild-type CDI(+) cells in both laboratory media and upon a eukaryotic host. Taken together, these results suggest that CDI systems constitute an intricate immunity network that plays an important role in bacterial competition. 2010-11-18 /pmc/articles/PMC3058911/ /pubmed/21085179 http://dx.doi.org/10.1038/nature09490 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Aoki, Stephanie K.
Diner, Elie J.
de Roodenbeke, Claire t'Kint
Burgess, Brandt R.
Poole, Stephen J.
Braaten, Bruce A.
Jones, Allison M.
Webb, Julia S.
Hayes, Christopher S.
Cotter, Peggy A.
Low, David A.
A widespread family of polymorphic contact-dependent toxin delivery systems in bacteria
title A widespread family of polymorphic contact-dependent toxin delivery systems in bacteria
title_full A widespread family of polymorphic contact-dependent toxin delivery systems in bacteria
title_fullStr A widespread family of polymorphic contact-dependent toxin delivery systems in bacteria
title_full_unstemmed A widespread family of polymorphic contact-dependent toxin delivery systems in bacteria
title_short A widespread family of polymorphic contact-dependent toxin delivery systems in bacteria
title_sort widespread family of polymorphic contact-dependent toxin delivery systems in bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058911/
https://www.ncbi.nlm.nih.gov/pubmed/21085179
http://dx.doi.org/10.1038/nature09490
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